Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

Liliana JARDA, (Al. Borza Botanical Garden, Babeş-Bolyai University, Cluj Napoca, Romania)
Anca BUTIUC-KEUL, (Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeş-Bolyai University, Cluj Napoca, Romania)
Maria HÖHN, (Department of Botany, Faculty of Horticultural Science, Corvinus University, Budapest, Hungary)
Andrzej PEDRYC, (Department of Genetics and Plant Breeding, Faculty of Horticultural Science, Corvinus University, Budapest, Hungary)
Victoria CRISTEA (Al. Borza Botanical Garden, Babeş-Bolyai University, Cluj Napoca, Romania)
Turkish Journal of Biology
1 0
Yıl: 2014 Cilt: 38 Sayı: 1 Sayfa Aralığı: 21 - 30 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

Öz:
Ex situ conservation of Dianthus giganteus subsp. banaticus was obtained by in vitro culture on different culture media supplemented with phytohormones. Assessment of somaclonal variability over a period of preservation by in vitro culture is required prior to introduction and acclimatization in natural habitats. In this study we used 2 types of molecular markers, ISSR and multilocus SSR markers, which allow comparison of different alleles, to assess the somaclonal variability of endemic taxa Dianthus giganteus subsp. banaticus after a period of 24 months of preservation by in vitro culture. The assessment of somaclonal variability of D. giganteus subsp. banaticus shows some changes in the absence/presence of certain markers in some individuals.
Anahtar Kelime:

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA JARDA L, Butiuc-Keul A, HÖHN M, PEDRYC A, CRISTEA V (2014). Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers. , 21 - 30.
Chicago JARDA Liliana,Butiuc-Keul Anca,HÖHN Maria,PEDRYC Andrzej,CRISTEA Victoria Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers. (2014): 21 - 30.
MLA JARDA Liliana,Butiuc-Keul Anca,HÖHN Maria,PEDRYC Andrzej,CRISTEA Victoria Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers. , 2014, ss.21 - 30.
AMA JARDA L,Butiuc-Keul A,HÖHN M,PEDRYC A,CRISTEA V Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers. . 2014; 21 - 30.
Vancouver JARDA L,Butiuc-Keul A,HÖHN M,PEDRYC A,CRISTEA V Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers. . 2014; 21 - 30.
IEEE JARDA L,Butiuc-Keul A,HÖHN M,PEDRYC A,CRISTEA V "Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers." , ss.21 - 30, 2014.
ISNAD JARDA, Liliana vd. "Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers". (2014), 21-30.
APA JARDA L, Butiuc-Keul A, HÖHN M, PEDRYC A, CRISTEA V (2014). Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers. Turkish Journal of Biology, 38(1), 21 - 30.
Chicago JARDA Liliana,Butiuc-Keul Anca,HÖHN Maria,PEDRYC Andrzej,CRISTEA Victoria Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers. Turkish Journal of Biology 38, no.1 (2014): 21 - 30.
MLA JARDA Liliana,Butiuc-Keul Anca,HÖHN Maria,PEDRYC Andrzej,CRISTEA Victoria Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers. Turkish Journal of Biology, vol.38, no.1, 2014, ss.21 - 30.
AMA JARDA L,Butiuc-Keul A,HÖHN M,PEDRYC A,CRISTEA V Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers. Turkish Journal of Biology. 2014; 38(1): 21 - 30.
Vancouver JARDA L,Butiuc-Keul A,HÖHN M,PEDRYC A,CRISTEA V Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers. Turkish Journal of Biology. 2014; 38(1): 21 - 30.
IEEE JARDA L,Butiuc-Keul A,HÖHN M,PEDRYC A,CRISTEA V "Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers." Turkish Journal of Biology, 38, ss.21 - 30, 2014.
ISNAD JARDA, Liliana vd. "Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers". Turkish Journal of Biology 38/1 (2014), 21-30.
Sayfa Oluşturulma Tarihi
17-04-2024 1:39

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