Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility

SOYLU, M. Kemal; SAĞIRKAYA, Hakan; ÜSTÜNER, BURCU; NUR, ZEKARIYA; ALÇAY, SELİM

Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility

Burcu ÜSTÜNER, (Uludağ Üniversitesi, Veteriner Fakültesi, Bursa, Türkiye)

Selim ALÇAY, (Uludağ Üniversitesi, Veteriner Fakültesi, Bursa, Türkiye)

Zekariya NUR, (Uludağ Üniversitesi, Veteriner Fakültesi, Bursa, Türkiye)

Hakan SAĞIRKAYA, (Uludağ Üniversitesi, Veteriner Fakültesi, Bursa, Türkiye)

M. Kemal SOYLU (Uludağ Üniversitesi, Veteriner Fakültesi, Bursa, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

2 0

Yıl: 2014 Cilt: 20 Sayı: 3 Sayfa Aralığı: 393 - 398 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility

Öz:

Bu çalışmanın amacı, yumurta sarısı ve farklı lesitin konsantrasyonlarının koç spermasının dondurulabilirliği üzerine etkisini değerlendirmek ve dondurup çözdürülen koç spermasının fertilizasyon yeteneğini tespit etmektir. Kitle hareketi (3-5) ve >%70 motiliteye sahip ejakülatlar birleştirildi (pooling). Pooling yapılan sperma dört gruba bölündü ve %1, %3, %6 (L1, L3 ve L6) lesitin veya %20 yumurta sarısı (EY20) içeren sulandırıcılar ile 1/5 (sperma/sulandırıcı) oranında iki aşamalı sulandırma yöntemi kullanılarak sulandırıldı. Bu çalışmanın sonucunda; motilite ve akrozomal bozukluk oranlarının dondurma prosedüründen olumsuz yönde etkilendiği tespit edildi (P<0.001). EY20 grubunun 5°C’de ve eritme sonrası motilite değerleri L1, L3 ve L6 gruplarına göre yüksek bulundu (P<0.05). Sulandırma, soğutma, ekilibrasyon ve eritme sonrası aşamalarda motilite oranları bakımından lesitin grupları arasında farklılık saptanmadı (P>0.05). Blastosist oranları bakımından değerlendirilen in vitro fertilizasyon sonuçlarına göre, EY20 grubunun farklı lesitin gruplarından daha başarılı olduğu tespit edildi. Sonuç olarak, yumurta sarısı içeren sulandırıcı ile dondurulan koç spermasının lesitin gruplarına göre eritme sonrası spermatolojik özellikler ve embriyonik gelişim bakımından daha üstün olduğu tespit edildi.

Anahtar Kelime:

Konular: Veterinerlik

Tris-bazlı sulandırıcılarda yumurta sarısı ve soya lesitininin eritme sonrası koç spermasının kalitesi ve in vitro fertilite üzerine etkisi

Öz:

The aim of the current study was to evaluate the effect of egg yolk and different soybean lecithin concentrations on the efficiency of ram semen cryopreservation and to test the fertilizing ability of frozen-thawed ram semen. Ejaculates with a thick consistency, rapid wave motion (3-5), and >70% initial motility were pooled. Pooled semen were then divided into four groups and diluted at 1/5 (semen/extender) with 1%, 3%, 6% lecithin (L1, L3 and L6) or 20% egg yolk (EY20) using the two-step dilution method. As expected, the results of the current study showed that both motility and the rates of defective acrosomes in sperm were negatively affected by the cryopreservation procedure (P<0.001). The motility values of at 5°C and post-thawed semen in the EY20 group were significantly higher than those in the L1, L3 and L6 groups (P<0.05). There were no differences in motility rates among the lecithin groups at the dilution, cooling, equilibration or post thawing stages (P>0.05). The results of in vitro fertilization, as assessed by the rate of blastocyst formation, were more successful in the EY20 group than those noted in different lecithin groups. In conclusion, freezing ram semen with an extender containing egg yolk could yield better post-thaw sperm parameters and embryonic development compared to lecithin containing extenders.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ÜSTÜNER B, ALÇAY S, NUR Z, SAĞIRKAYA H, SOYLU M (2014). Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. , 393 - 398.
Chicago	ÜSTÜNER BURCU,ALÇAY SELİM,NUR ZEKARIYA,SAĞIRKAYA Hakan,SOYLU M. Kemal Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. (2014): 393 - 398.
MLA	ÜSTÜNER BURCU,ALÇAY SELİM,NUR ZEKARIYA,SAĞIRKAYA Hakan,SOYLU M. Kemal Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. , 2014, ss.393 - 398.
AMA	ÜSTÜNER B,ALÇAY S,NUR Z,SAĞIRKAYA H,SOYLU M Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. . 2014; 393 - 398.
Vancouver	ÜSTÜNER B,ALÇAY S,NUR Z,SAĞIRKAYA H,SOYLU M Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. . 2014; 393 - 398.
IEEE	ÜSTÜNER B,ALÇAY S,NUR Z,SAĞIRKAYA H,SOYLU M "Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility." , ss.393 - 398, 2014.
ISNAD	ÜSTÜNER, BURCU vd. "Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility". (2014), 393-398.

APA	ÜSTÜNER B, ALÇAY S, NUR Z, SAĞIRKAYA H, SOYLU M (2014). Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 20(3), 393 - 398.
Chicago	ÜSTÜNER BURCU,ALÇAY SELİM,NUR ZEKARIYA,SAĞIRKAYA Hakan,SOYLU M. Kemal Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 20, no.3 (2014): 393 - 398.
MLA	ÜSTÜNER BURCU,ALÇAY SELİM,NUR ZEKARIYA,SAĞIRKAYA Hakan,SOYLU M. Kemal Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.20, no.3, 2014, ss.393 - 398.
AMA	ÜSTÜNER B,ALÇAY S,NUR Z,SAĞIRKAYA H,SOYLU M Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2014; 20(3): 393 - 398.
Vancouver	ÜSTÜNER B,ALÇAY S,NUR Z,SAĞIRKAYA H,SOYLU M Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2014; 20(3): 393 - 398.
IEEE	ÜSTÜNER B,ALÇAY S,NUR Z,SAĞIRKAYA H,SOYLU M "Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 20, ss.393 - 398, 2014.
ISNAD	ÜSTÜNER, BURCU vd. "Effect of egg yolk and soybean lecithin on tris-based extender in post-thaw ram semen quality and in vitro fertility". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 20/3 (2014), 393-398.