Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus

ARSLAN, Hülya; Güleryüz, Gürcan; KIRMIZI, Serap

Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus

Serap KIRMIZI, (Uludağ Üniversitesi, Asım Kocabıyık Meslek Yüksekokulu, Bursa, Türkiye)

Gürcan GÜLERYÜZ, (Uludağ Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Bursa, Türkiye)

Hülya ARSLAN (Uludağ Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Bursa, Türkiye)

Turkish Journal of Botany

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Yıl: 2014 Cilt: 38 Sayı: 2 Sayfa Aralığı: 302 - 308 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus

Öz:

We studied protein mobilisation in Asphodelus aestivus Brot. (Liliaceae) during a 1-year period. The activities of 3 proteolytic enzymes, endopeptidase (EP), leucine aminopeptidase (LAP), and carboxypeptidase (CP), together with soluble protein levels and total nitrogen and water content in root tubers were investigated. We found that protein mobilisation continued throughout the year. The highest levels of the 3 proteolytic enzymes were found in different months: LAP in December (1.19 ± 0.19 μmol pNA/g FW), CP in February (14.19 ± 1.13 μmol Leu/g FW), and EP in May (5.18 ± 0.12 U/h). Activities of LAP and CP generally reached their higher levels in January–April, namely in spring, but EP activity was higher in March–May. Activities of all 3 enzymes remained at lower levels during the asphodel’s inactive life phase. The soluble protein contents were associated with the proteolytic activities. The total N contents were generally constant, but decreased in April. Our results show that the proteolytic activities in root tubers of Asphodelus aestivus continue to increase up to the start of flowering in early spring, and they can be an important nitrogen resource at the beginning of the generative development phase.

Anahtar Kelime:

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

Aschmann H (1973). Distribution and peculiarity of Mediterranean ecosystem. In: Di Castri F, Mooney HA, editors. Mediterranean Type Ecosystems. Berlin, Germany: Springer, pp. 11–19.
Ayyad M, Hilmy SH (1974). The distribution of Asphodelus microcarpus and associated species on the western Mediterranean coast of Egypt. Ecology 55: 511–524.
Bewley JD (2002). Root storage proteins, with particular reference to taproots. Can J Bot 80: 321–329.
Bewley JD, Black M (1982). Physiology and Biochemistry of Seeds in Relation to Germination. Vol. 1. Berlin, Germany: Springer.
Bradford M (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248–256.
Callis J (1995). Regulation of protein degradation. Plant Cell 7: 845– 857.
Chin T, Poulson R, Beevers L (1972). The influence of axis removal on protein metabolism in cotyledons of Pisum sativum L. Plant Physiol 49:482–489.
Cyr DR, Bewley JD (1989). Carbon and nitrogen reserves of leafy spurge (Euphorbia esula) roots as related to overwintering strategy. Physiol Plant 77: 67–72.
Cyr DR, Bewley JD (1990a). Proteins in the roots of the perennial weeds chicory (Cichorium intybus L.) and dandelion (Taraxacum officinale Weber) are associated with overwintering. Planta 182: 370–374.
Cyr DR, Bewley JD (1990b). Seasonal dynamics of carbohydrate and nitrogenous components in the roots of perennial weeds. Plant Cell Environ 13: 359–365.
Dafni A (1996). Autumnal and winter pollination adaptations under Mediterranean conditions. Bocconea 5: 171–181.
Dafni A, Cohen D, Noy-Meir I (1981a). Life cycle variation in geophytes. Ann Mo Bot Gard 68: 652–660.
Dafni A, Shmida A, Avishai M (1981b). Leafless autumnal flowering geophytes in the Mediterranean region: phytogeographical, ecological and evolutionary aspects. Plant Syst Evol 137: 181– 193.
Debussche M, Garnier E, Thompson JD (2004). Exploring the causes of variation in phenology and morphology in Mediterranean geophytes: a genus-wide study of Cyclamen. Bot J Linn Soc 145: 469–484.
De Hertogh AA, Le Nard M (1993). Physiological and biochemical aspects of flower bulbs. In: De Hertogh AA, Le Nard M, editors. The Physiology of Flower Bulbs. Amsterdam, the Netherlands: Elsevier, pp. 53–69.
Franková L, Cibírová K, Bόka C, Gašparíková O, Pšená M (2006). Protein reutilization in corms of Colchicum autumnale. Biol Brat 31: 97–102.
Guimaráes RL, Marcellino LH, Grossi De Sa MF, De Castro Monte D (2001). A storage protein gene from taro shows tuber-specific expression in transgenic potato. Physiol Plant 111: 182–187.
Hüseyinova R, Kılınç M, Kutbay HG, Kılıç DD, Bilgin A (2013). The comparison of Grime’s strategies of plant taxa in Hacı Osman forest and Bafra Fish Lakes in the central Black Sea region of Turkey. Turk J Bot 37: 725–734.
Kamenetsky R, Peterson R, Melville LH, Machado CF, Bewley JD (2005). Seasonal adaptations of the tuberous roots of Ranunculus asiaticus to desiccation and resurrection by changes in cell structure and protein content. New Phytol 166: 193–204.
Kırmızı S, Güleryüz G (2006). Protein mobilization and proteolytic enzyme activities during seed germination of broad bean (Vicia faba L.). Z Naturforsch C61: 222–226.
Kırmızı S, Güleryüz G (2007). Monitoring protein mobilization during seed germination of broad bean (Vicia faba L.). Asian J Plant Sci 6: 374–378.
Le Houerou HN (1981). Impact of man and his animals on Mediterranean vegetation. In: Di Castri F, Gooddall DW, Specht R, editors. Ecosystems of the World, Mediterranean- Type Shrublands. Vol. II. Amsterdam, the Netherlands: Elsevier: pp. 479–521.
Lin YH, Yao WH (1995). Protease activities before and after germination of garlic (Allium sativum L.) bulbs. Bot Bull Acad Sin 36: 189–194.
Miller WB, Legnani G, Ranwala AP, Hardin MB (1997). Fructan metabolism in geophytes. Acta Hort 430: 117–124.
Mitsuhashi W, Koshiba T, Minamikawa T (1984). Influence of axis removal on amino, carboxy-, and endopeptidase activities in cotyledons of germinating Vigna mungo seeds. Plant Cell Physiol 25: 547–554.
Munoz JL, Martin L, Nicholas G, Villalobos N (1990). Influence of endogenous cytokinins on reverse mobilization in cotyledons of Cicer arietinum L. Plant Physiol 93: 1011–1016.
Naveh Z (1973). The ecology of fire in Israel. In: Proceedings 13th Tall Timber Fire Ecology Conference, Tallahassee, FL, USA, pp. 139–170.
Pantis JD (1993). Biomass and nutrient allocation patterns in the Mediterranean geophyte Asphodelus aestivus Brot. (Thessaly, Greece). Acta Ecol 14: 489–500.
Pantis J, Margaris NS (1988). Can systems dominated by asphodels be considered as semi-deserts? Int J Biometeorol 32: 87–91.
Pantis JD, Sgardelis SP, Stamou GP (1994). Asphodelus aestivus, an example of synchronization with the climate periodicity. Int J Biometeorol 38: 29–32.
Pirdal M (1989). Studies on the autecology of Asphodelus aestivus. Doğa Türk Botanik Dergisi 13: 89–101.
Raunkiaer C (1934). The Life Forms of Plants and Statistical Plant Geography. Oxford, UK: Clarendon Press.
Rehder H, Gökçeoğlu M, Gebauer G, Güleryüz G (1994). The vegetation of the Uludag Mountains (Anatolia). Phytocoenologia 24: 167–192.
Rhizopulou S, Pantis JD, Triantafylli E, Vokou D (1997). Ecophysiological adaptations of Asphodelus aestivus to Mediterranean climate periodicity: water relations and energetic status. Ecography 20: 626–633.
Sakar FS, Arslan H, Kırmızı S, Güleryüz G (2010). Nitrate reductase activity (NRA) in Asphodelus aestivus Brot. (Liliaceae): distribution among organs, seasonal variation and differences among populations. Flora 205: 527–531.
Sawidis T, Kalyva S, Delivopoulos S (2005). The root tuber anatomy of Asphodelus aestivus. Flora 200: 332–338.
Shewry PR (2003). Tuber storage proteins. Ann Bot 91: 755–769. Steubing L (1965). Pflanzen Ökologisches Praktikum. Berlin, Germany: Parey (in German).
Vardar F, İsmailoğlu I, Ünal M (2013). Anther development and cytochemistry in Asphodelus aestivus (Asphodelaceae). Turk J Bot 37: 306–315.

APA	KIRMIZI S, Güleryüz G, ARSLAN H (2014). Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus. , 302 - 308.
Chicago	KIRMIZI Serap,Güleryüz Gürcan,ARSLAN Hülya Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus. (2014): 302 - 308.
MLA	KIRMIZI Serap,Güleryüz Gürcan,ARSLAN Hülya Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus. , 2014, ss.302 - 308.
AMA	KIRMIZI S,Güleryüz G,ARSLAN H Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus. . 2014; 302 - 308.
Vancouver	KIRMIZI S,Güleryüz G,ARSLAN H Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus. . 2014; 302 - 308.
IEEE	KIRMIZI S,Güleryüz G,ARSLAN H "Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus." , ss.302 - 308, 2014.
ISNAD	KIRMIZI, Serap vd. "Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus". (2014), 302-308.

APA	KIRMIZI S, Güleryüz G, ARSLAN H (2014). Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus. Turkish Journal of Botany, 38(2), 302 - 308.
Chicago	KIRMIZI Serap,Güleryüz Gürcan,ARSLAN Hülya Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus. Turkish Journal of Botany 38, no.2 (2014): 302 - 308.
MLA	KIRMIZI Serap,Güleryüz Gürcan,ARSLAN Hülya Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus. Turkish Journal of Botany, vol.38, no.2, 2014, ss.302 - 308.
AMA	KIRMIZI S,Güleryüz G,ARSLAN H Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus. Turkish Journal of Botany. 2014; 38(2): 302 - 308.
Vancouver	KIRMIZI S,Güleryüz G,ARSLAN H Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus. Turkish Journal of Botany. 2014; 38(2): 302 - 308.
IEEE	KIRMIZI S,Güleryüz G,ARSLAN H "Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus." Turkish Journal of Botany, 38, ss.302 - 308, 2014.
ISNAD	KIRMIZI, Serap vd. "Protein mobilisation and proteolytic activities in root tubers of Asphodelus aestivus". Turkish Journal of Botany 38/2 (2014), 302-308.