A 3-year-old shorthair queen was referred to the animal hospital in July with the complaint of dystocia that started five days ago. Abdominal ultrasonography revealed that no structures related to fetus or fetal fuid were evident however, radiography of pelvic axis showed fetus-related bone opacities. On vaginal examination, a reddish-brown, fetid and slimy vaginal discharge and a total of eleven larvae were observed and the all larvae were transported to Parasitology laboratory. On exploratory laparotomy, two dead fetuses were seen throughout the corpus-cervix uteri, therefore ovariohysterectomie was performed after the approval of the owner. The morphology of Dipteran fly larvae and the adult flies which were emerged from larvae fed in insectarium provided evidence that Lucilia sericata was responsible for the genital myiasis. It is suggested that vulvo-vaginal myiasis should take into account by veterinary practitioners following prolonged dystocia cases, especially occurring in poor environmental conditions in hot seasons.