Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity

Esra OZBEK, (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Kimya Bölümü, Trabzon, Türkiye)
YAKUP KOLCUOĞLU, (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Kimya Bölümü, Trabzon, Türkiye)
Leyla KONAK, (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Kimya Bölümü, Trabzon, Türkiye)
Ahmet ÇOLAK, (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Kimya Bölümü, Trabzon, Türkiye)
Fulya ÖZ (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Kimya Bölümü, Trabzon, Türkiye)
Turkish Journal of Chemistry
0 0
Yıl: 2014 Cilt: 38 Sayı: 4 Sayfa Aralığı: 538 - 546 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity

Öz:
An esterase from a thermophilic bacterium, Geobacillus sp. DF20, was partially purified. Final purification factor was found to be 64.5-fold using Q-Sepharose ion exchange column chromatography. Native polyacrylamide gel electrophoresis indicated the presence of a single active esterase. The substrate specificity of this esterase was high for p -nitrophenyl butyrate ( p NPB) substrate. The optimum pH and temperature for the enzyme activity were 7.0 and 50 ◦C, respectively. The pH and heat stability profiles show that this enzyme is more stable under neutral conditions at 50 ◦C. Km and Vmax values for this esterase acting on p NPB were 0.12 mM and 54.6 U/mg protein, respectively. Presence of 10% (v/v) acetonitrile in the reaction medium indicated that purified enzyme was strongly inhibited. It was also detected that some metal ions affected enzyme activity at different rates. As a result, it was observed that esterase from Geobacillus sp. DF20 has extreme temperature and pH stabilities. Therefore, the stability and Km value of the enzyme make this study interesting when compared with the literature.
Anahtar Kelime:

Konular: Mühendislik, Kimya
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA OZBEK E, KOLCUOĞLU Y, KONAK L, Çolak A, ÖZ F (2014). Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity. , 538 - 546.
Chicago OZBEK Esra,KOLCUOĞLU YAKUP,KONAK Leyla,Çolak Ahmet,ÖZ Fulya Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity. (2014): 538 - 546.
MLA OZBEK Esra,KOLCUOĞLU YAKUP,KONAK Leyla,Çolak Ahmet,ÖZ Fulya Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity. , 2014, ss.538 - 546.
AMA OZBEK E,KOLCUOĞLU Y,KONAK L,Çolak A,ÖZ F Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity. . 2014; 538 - 546.
Vancouver OZBEK E,KOLCUOĞLU Y,KONAK L,Çolak A,ÖZ F Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity. . 2014; 538 - 546.
IEEE OZBEK E,KOLCUOĞLU Y,KONAK L,Çolak A,ÖZ F "Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity." , ss.538 - 546, 2014.
ISNAD OZBEK, Esra vd. "Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity". (2014), 538-546.
APA OZBEK E, KOLCUOĞLU Y, KONAK L, Çolak A, ÖZ F (2014). Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity. Turkish Journal of Chemistry, 38(4), 538 - 546.
Chicago OZBEK Esra,KOLCUOĞLU YAKUP,KONAK Leyla,Çolak Ahmet,ÖZ Fulya Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity. Turkish Journal of Chemistry 38, no.4 (2014): 538 - 546.
MLA OZBEK Esra,KOLCUOĞLU YAKUP,KONAK Leyla,Çolak Ahmet,ÖZ Fulya Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity. Turkish Journal of Chemistry, vol.38, no.4, 2014, ss.538 - 546.
AMA OZBEK E,KOLCUOĞLU Y,KONAK L,Çolak A,ÖZ F Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity. Turkish Journal of Chemistry. 2014; 38(4): 538 - 546.
Vancouver OZBEK E,KOLCUOĞLU Y,KONAK L,Çolak A,ÖZ F Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity. Turkish Journal of Chemistry. 2014; 38(4): 538 - 546.
IEEE OZBEK E,KOLCUOĞLU Y,KONAK L,Çolak A,ÖZ F "Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity." Turkish Journal of Chemistry, 38, ss.538 - 546, 2014.
ISNAD OZBEK, Esra vd. "Partial purification and biochemical characterization of an extremely thermo- and pH-stable esterase with great substrate affinity". Turkish Journal of Chemistry 38/4 (2014), 538-546.
Sayfa Oluşturulma Tarihi
22-04-2024 20:25

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