TY  - JOUR
TI  - Rosmarinic acid: a potent carbonic anhydrase isoenzymes inhibitor
AB  - Rosmarinic acid is a water-soluble ester of caffeic acid and 3,4-dihydroxyphenyllactic acids, and is mainly found in plant species including Boraginaceae and Lamiaceae. In this research, we determined the inhibition property of rosmarinic acid on carbonic anhydrase isoenzymes I and II (hCA I and II) purified from human erythrocytes by using Sepharose-4B affinity column chromatography. hCA I and II isoenzymes were obtained with a yield of 57.9% and 67.2% and 76.5- and 509.3-fold purification of each isoenzyme, respectively. In order to show the purity of the isoenzymes, SDS-PAGE was performed and one band was observed. In vitro inhibition of both hCA I and II isoenzymes by rosmarinic acid using CO 2 -esterase activity gave Ki values of 86.0 μ M and 57.0 μ M, respectively.
AU  - Topal, Meryem
AU  - Gulcin, ilhami
PY  - 2014
JO  - Turkish Journal of Chemistry
VL  - 38
IS  - 5
SN  - 1300-0527
SP  - 894
EP  - 902
DB  - TRDizin
UR  - http://search/yayin/detay/163488
ER  -