Effects of various cryoprotective agents on post-thaw drone semen quality

ÇAKMAK, Selvinar; NUR, ZEKARIYA; ÜSTÜNER, BURCU; ALÇAY, SELİM; Cakmak, Ibrahim

Effects of various cryoprotective agents on post-thaw drone semen quality

Selim ALÇAY, (Uludağ Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Burcu ÜSTÜNER, (Uludağ Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

İbrahim ÇAKMAK, (Uludağ Üniversitesi, Mustafa Kemalpaşa Meslek Yüksek Okulu, Bursa, Türkiye)

Selvinar ÇAKMAK, (Uludağ Üniversitesi, Arıcılık Geliştirme Uygulama ve Araştırma Merkezi AGAM, Bursa, Türkiye)

Zekariya NUR (Uludağ Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2015 Cilt: 21 Sayı: 1 Sayfa Aralığı: 31 - 35 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effects of various cryoprotective agents on post-thaw drone semen quality

Öz:

Bu çalışmanın amacı, farklı kriyoprotektanların eritme sonrası arı spermasının motilite ve plazma membran bütünlüğü üzerineetkisini değerlendirmektir. Sperma dört koloniden bulunan olgun arılardan (16 gün ve üzeri) alındı. Toplanan sperma finalkonsantrasyonu 1/5 olacak şekilde %0 kriyoprotektan içermeyen (kontrol), %6 gliserol, %6 etilen glikol ve %6 1,2 Propanediol ileiki aşamalı sulandırıldı. Ekilibrasyon sonrası sperma 0.25 mllik payetlerde donduruldu. Sulandırma sonrası, ekilibrasyon ve eritmesonrası aşamalarda motilite (%) ve HOS testi yapıldı. Eritme sonrası motilite ve plazma membran bütünlüğüne bakıldığında kullanımöncülüğüne göre kriyoprotektanları DMSO, Etilen Glikol, Gliserol ve 1,2 Propanediol olarak sıralayabiliriz. Sonuç olarak, eritme sonrasımotilite ve plazma membran bütünlüğü bakımından; DMSO ile dondurulan sperma, kontrol, gliserol, etilen glikol, 1,2 propanediolgruplarıyla karşılaştırıldığında en iyi sonucu vermiştir (P<0.05).

Anahtar Kelime:

Konular: Veterinerlik

Farklı kriyoprotektanların eritme sonrası arı sperm kalitesi üzerine etkileri

Öz:

The aim of the present study was to evaluate the efect of diferent cryoprotectants on post-thaw semen motility and plasma membraneintegrity of drone spermatozoa. Semen was obtained from mature drones (16 days or older) harvested from four colonies. Collectedsemen was diluted to a fnal concentration of 1/5 (semen/extender) in 0% cryoprotectant (control), 6% glycerol, 6% Ethylene Glycol, 6%1,2 propanediol or 6% DMSO using a two-step dilution method. The equilibrated semen was frozen in 0.25-ml straws. The percentageof sperm motility and swollen tails (HOST) spermatozoa were evaluated following dilution with extender A (non-cryoprotectant),equilibration and post-thaw stages. In terms of post-thaw motility and plasma membrane integrity recovery we can rank the usedcryoprotectant as DMSO, Ethylene Glycol, Glycerol and 1,2 Propanediol; respectively. In conclusion, post-thaw sperm motility andplasma membrane integrity of the present study was signifcantly better when sperm was frozen in DMSO with respect to control,glycerol, ethylene glycol, 1,2 propanediol (P<0.05).

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ALÇAY S, ÜSTÜNER B, Cakmak I, ÇAKMAK S, NUR Z (2015). Effects of various cryoprotective agents on post-thaw drone semen quality. , 31 - 35.
Chicago	ALÇAY SELİM,ÜSTÜNER BURCU,Cakmak Ibrahim,ÇAKMAK Selvinar,NUR ZEKARIYA Effects of various cryoprotective agents on post-thaw drone semen quality. (2015): 31 - 35.
MLA	ALÇAY SELİM,ÜSTÜNER BURCU,Cakmak Ibrahim,ÇAKMAK Selvinar,NUR ZEKARIYA Effects of various cryoprotective agents on post-thaw drone semen quality. , 2015, ss.31 - 35.
AMA	ALÇAY S,ÜSTÜNER B,Cakmak I,ÇAKMAK S,NUR Z Effects of various cryoprotective agents on post-thaw drone semen quality. . 2015; 31 - 35.
Vancouver	ALÇAY S,ÜSTÜNER B,Cakmak I,ÇAKMAK S,NUR Z Effects of various cryoprotective agents on post-thaw drone semen quality. . 2015; 31 - 35.
IEEE	ALÇAY S,ÜSTÜNER B,Cakmak I,ÇAKMAK S,NUR Z "Effects of various cryoprotective agents on post-thaw drone semen quality." , ss.31 - 35, 2015.
ISNAD	ALÇAY, SELİM vd. "Effects of various cryoprotective agents on post-thaw drone semen quality". (2015), 31-35.

APA	ALÇAY S, ÜSTÜNER B, Cakmak I, ÇAKMAK S, NUR Z (2015). Effects of various cryoprotective agents on post-thaw drone semen quality. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(1), 31 - 35.
Chicago	ALÇAY SELİM,ÜSTÜNER BURCU,Cakmak Ibrahim,ÇAKMAK Selvinar,NUR ZEKARIYA Effects of various cryoprotective agents on post-thaw drone semen quality. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21, no.1 (2015): 31 - 35.
MLA	ALÇAY SELİM,ÜSTÜNER BURCU,Cakmak Ibrahim,ÇAKMAK Selvinar,NUR ZEKARIYA Effects of various cryoprotective agents on post-thaw drone semen quality. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.21, no.1, 2015, ss.31 - 35.
AMA	ALÇAY S,ÜSTÜNER B,Cakmak I,ÇAKMAK S,NUR Z Effects of various cryoprotective agents on post-thaw drone semen quality. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(1): 31 - 35.
Vancouver	ALÇAY S,ÜSTÜNER B,Cakmak I,ÇAKMAK S,NUR Z Effects of various cryoprotective agents on post-thaw drone semen quality. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(1): 31 - 35.
IEEE	ALÇAY S,ÜSTÜNER B,Cakmak I,ÇAKMAK S,NUR Z "Effects of various cryoprotective agents on post-thaw drone semen quality." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21, ss.31 - 35, 2015.
ISNAD	ALÇAY, SELİM vd. "Effects of various cryoprotective agents on post-thaw drone semen quality". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21/1 (2015), 31-35.