The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen

OZTÜRKLER, YAVUZ; ATAKİŞİ, Emine; ARI, Umut Çağın; KULAKSIZ, Recai; YILDIZ, Savaş; LEHİMCİOĞLU, NECDET CANKAT

The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen

Savaş YILDIZ, (Kafkas Üniversitesi, Veteriner Fakültesi, Kars, Türkiye)

Yavuz ÖZTÜRKLER, (Kafkas Üniversitesi, Veteriner Fakültesi, Kars, Türkiye)

Umut Çağın ARI, (Kafkas Üniversitesi, Veteriner Fakültesi, Kars, Türkiye)

Necdet Cankat LEHİMCİOĞLU, (Kafkas Üniversitesi, Veteriner Fakültesi, Kars, Türkiye)

Emine ATAKİŞİ, (Kafkas Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Kars, Türkiye)

Recai KULAKSIZ (Kafkas Üniversitesi, Veteriner Fakültesi, Kars, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

5 0

Yıl: 2015 Cilt: 21 Sayı: 1 Sayfa Aralığı: 81 - 86 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen

Öz:

Bu çalışmanın amacı, koçlarda L-Ergothionin (LE), sistein ve N-asetil sisteinin (NAC) farklı dozlarının çözüm sonu sperma parametreleriüzerine etkisini belirlemekti. Dört Tuj ırkı koçtan alınan ejakülatlar değerlendirildi ve 35°Cde karışım yapıldı. Sperma örnekleri, LE (5, 10mM), Sistein (5, 10 mM), NAC (5, 10 mM) içeren veya antioksidan içermeyen (kontrol), yağsız süt temelli sulandırıcıyla sulandırılarak, 0.25mllik payetlere dolduruldu. Payetler 5°Cye 2 saat süreyle soğutuldu, sıvı azot buharında (yaklaşık -120°C) 15 dak. süre ile dondurulduve çözme işlemine kadar sıvı azot içinde saklandı. Payetler su banyosunda (37°Cde 1 dak.) çözüldü. Motilite, canlılık, anormal akrozom,toplam anormalite ve membran bütünlüğü (hipoozmotik şişme testi, HOST) yüzdeleri istatistiksel olarak değerlendirildi. Ayrıca,toplam antioksidan kapasite (TAK) ve toplam oksidatif stres (TOS), replikasyonlardan elde edilen örneklerde değerlendirildi. LEninmotilite (sistein hariç), canlılık, akrozom hasarı, total morfolojik anormalite, HOST ve TAK oranları bakımından, NAC ve sisteine üstünlüksağladığı görüldü (P<0.05). Yine de, LE ve kontrol grubu arasında istatistiksel bir fark yoktu (P>0.05). Sistein ve NACın koçlarda çözümsonu spermatolojik parametreler üzerine bazı olumsuz etkileri belirlenirken, LEnin olumlu ya da olumsuz etkisinin olmadığı sonucunavarıldı. Bu sonuçlar, koç spermasının dondurulmasında, LE, NAC ve sistein kullanılması üzerine yeni bilimsel çalışmaların yapılmasıgerektiğini göstermektedir.

Anahtar Kelime:

Konular: Veterinerlik

Koç spermasının dondurulması üzerine L-Ergothionin, N-asetil sistein ve sisteinin etkileri

Öz:

The aim of this study was to determine the infuence of diferent doses of L-Ergothioneine (LE), cystein and N-acetylcystein (NAC)on post-thawing semen parameters in rams. Ejaculates collected from four Tushin Rams were evaluated and pooled at 35°C. Semensamples were diluted with skim-milk based extender containing LE (5, 10 mM), Cystein (5, 10 mM), NAC (5, 10 mM) or not containingany antioxidant (control) and loaded to 0.25 ml French straws. Straws were cooled to 5°C for 2 h, frozen in liquid nitrogen vapour(aprox. -120°C) for 15 min and then being stored in liquid nitrogen until thawing process. Straws were thawed in water bath (37°Cfor 1 min). The percentages of motility, viability, abnormal acrosome, total abnormalities, membrane integrity (hypoosmaticswelling test, HOST) were statistically assessed. Also total antioxidant capacity (TAC) and total oxidatif stres (TOS) were evaluated insamples from replications. It was seen that LE was superior to N-acetylcystein and cystein in motility, viability, defected acrosome,total morphological abnormalities, HOST and TAC, (P<0.05) except cystein in motility. Neverthless, there was not any statisticallydiference between LE and control groups (P>0.05). It was conlcluded that there was not any benefcial or detrimental efects of LEon post thawing semen parameters in rams while it was determined that cystein and NAC may have been some detrimental efectson post thawing semen parameters in rams. These results warrants future scientifc studies on LE, NAC and cystein in ram semencryopreservation.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Yıldız S, Daşkın A: Koç spermasının farklı antioksidanlar içeren sulandırıcılarla kısa süreli saklanması. Kafkas Univ Vet fak Derg, 10 (2): 155- 159, 2004.
2. Marti E, Mara L, Marti JI, Muiño-Blanco T, Cebrián-Pérez JA: Seasonal variations in antioxidant enzyme activity in ram seminal plasma. Theriogenology, 67 (9): 1446-1454, 2007.
3. Pagl R, Aurich JE, Müller-Schlösser F, Kankofer M, Aurich C: Comparison of an extender containing defined milk protein fractions with a skim milk-based extender for storage of equine semen at 5-8°C. Theriogenology, 66, 1115-1122, 2006.
4. Erokhin AS, Nikifirov GA, Epishina TM: Protective effect of new synthetic antioxidants in the cryopreservation of ram semen. Selskokhozyaistvennaya Biologiya , 6, 62-67, 1996.
5. Maxwell WMC, Stojanov T: Liquid storage of ram semen in the absence or presence of some antioxidants. Reprod Fertil Dev, 8 (6): 1013- 1020, 1996.
6. Marti JI, Marti E, Muino-Blanco T, Cebrian-Perez CA: Study on the freezing of ram semen. Variations in the antioxidant capacity during the process. ITEA, Extra, 22 (2): 853-855, 2001.
7. Neild DM, Brouwers JFHM, Colenbrander B, Agüero A, Gadella BM: Lipid peroxide formation in relation to membrane stability of fresh and frozen thawed stallion spermatozoa. Mol Reprod Dev, 72 (2): 230-238, 2005.
8. Kheradmand A, Babaei H, Abshenas J: Comparative evaluation of the efect of antioxidants on the chilled-stored ram semen. Iranian J Vet Res, 7 (4): 40-45, 2006.
9. Oeda T, Henkel R, Ohmori H, Schill WB: Scavenging efect of n-2007 acetyl-l-cysteine against reactive oxygen species in human semen: A possible therapeutic modality for male factor infertility? Andrologia, 29 (3): 125-231, 1997.
10. Nichi M, Goovaerts IGF, Cortada CNM, Barnabe VH, De Clercq JBP, Bols PEJ: Roles of lipid peroxidation and cytoplasmic droplets on ın vitro fertilization capacity of sperm collected from bovine epididymides stored at 4 and 34°C. Theriogenology, 67 (2): 334-340, 2007.
11. Hughes LCM, Lewis SEM, McKelvey-Martin VJ, Thompson W: The efects of antioxidant supplementation during percoll preparation on human sperm DNA integrity. Human Reprod, 13, 1240-1247, 1998.
12. Szczesnıak-Fabıanczy B, Bochenek M, Smorąg Z, Ryszka F: Efect of antioxidants added to boar semen extender on the semen survival time and sperm chromatin structure. Biol Reprod , 3 (1): 81, 2003.
13. Bucak MN, Ateşşahin A, Yüce A: Efect of anti-oxidants and oxidative stress parameters on ram semen after the freze-thawing process. Small Rum Res, 75 (2-3): 128-134, 2008.
14. Mitsuyama H, May JM: Uptake and antioxidant efects of ergothioneine in human erythrocytes. Clin Sci Lond , 97 (4): 407-411, 1999.
15. Guerin P, El Mouatassim S, Menezo Y: Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and ıts surroundings. Human Reprod Update, 7, 175-189, 2001.
16. Öztürkler Y, Yıldız S, Güngör Ö, Pancarcı ŞM, Kaçar C, Arı UÇ: The efects of L-ergothioneine and L-ascorbic acid on in vitro maturation and embryonic development in sheep oocytes. Kafkas Univ Vet Fak Derg, 16, 757-763, 2010.
17. Aruoma OI, Spencer JP, Mahmood R: Protection against oxidative damage and cell death by the natural antioxidant ergothioneine. Food Chem Toxicol, 37 (11): 1043-1053, 1999.
18. Akanmu D, Cecchini R, Aruoma OI, Halliwell B: The antioxidant action of ergothioneine. Arch Biochem Biophys, 288, 10-16, 1991.
19. Heath H, Larson A, Remington CJ, Searle C: Some efects of administrating ergothioneine to rats. Chem Soc, 50, 530-533, 1952.
20. Mann T, Leone E: Studies on the metabolism of semen, L-ergo- thioneine as a normal constituent of boar seminal plasma. Biochem J, 53, 140-148, 1953.
21. Aruoma OI, Whiteman M, England TG, Halliwell B: Antioxidant action of ergothioneine assessment of its ability to scavenge peroxynitrite. BBRC, 231, 389-391, 1997.
22. Haag FM, Mc Leod J: Relationship between nonprotein sulfhydryl concentration of seminal fuid and motility of spermatozoa in man. J Appl Physiol, 14, 27-30, 1959.
23. Aisen EG, Medina VH, Venturino A: Cryopreservation and post- thawed fertility of ram semen frozen in diferent trehalose concentrations. Theriogenology, 57, 1801-1808, 2002.
24. Gil J, Lundeheim N, Söderquist L, Rodriguez-Martinez H: Infuence of extender, temperature, and addition of glycerol on post-thaw sperm parameters in ram semen. Theriogenology, 59 (5-6): 1241-1255, 2003.
25. Bucak MN, Atessahin A, Varisli Ö, Yüce A, Tekin N, Akcay A: The infuence of trehalose, taurine, cysteamine and hyaluronan on ram semen microscopic and oxidative stress parameters after freeze-thawing process. Theriogenology, 67 (5): 1060-1067, 2007.
26. Evans G, Maxwell WMC: Handling and Examination Semen. In, Maxwell WMC (Ed): Salamons Artificial Insemination of Sheep and Goat. 93-106, Butterworths, Sydney, 1987.
27. Schafer S, Holzmann A: The use of transmigration and spermac stain to evaluate epididymal cat spermatozoa. Anim Reprod Sci, 59, 201-211, 2000.
28. Smith JT, Mayer DT: Evaluation of sperm concentration by the hemocytometer method. Fertil Steril, 6, 271-275, (1955).
29. Revell SG, Mrode RA: An osmotic resistance test for bovine semen. Anim Reprod Sci, 36, 77-86, 1994.
30. Buckett WM, Luckas MJ, Aird IA, Farquharson RG, Kingsland CR, Lewis-Jones DI: The hypo-osmotic swelling test in recurrent miscarriage. Fertil Steril, 68, 506-509, 1997.
31. Badarinath AV, RAo Mallikarjuna K, Madhu Sudhana Chetty C, Ramkanth S, Rajan TVS, Gnanaprakash K: A review on in-vitro antioxidant methods: comparisions, correlations and considerations. Int J Pharm Tech Res, 2 (2): 1276-1285, 2010.
32. Erel O: A New automated colorimetric method for measuring total antioxidant status. Clin Biochem, 38 (12): 1103-1111, 2005.
33. Daniel WW: Analysis of variance. In, Daniel WW (Ed): Biostatistic: A Foundation for Analysis in the Health Sciences. 274-320, John Wiley & Sons, Hoboken, 1991.
34. Çoyan K, Baspınar N, Bucak MN, Akalın PP: Efects of cysteine and ergothioneine on post-thawed merino ram sperm and biochemical parameters. Cryobiology, 63, 1-6, 2011.
35. Ari UÇ, Kulaksiz R, Ozturkler Y, Yildiz S, Lehimcioglu NC: Efect of L-(+)-Ergothioneine (EGT) on freezability of ram semen. Int J Anim Vet Adv, 4 (6): 378-383, 2012.
36. Bucak MN, Tuncer PB, Sarıozkan S, Ulutaş PA: Comparison of the efects of glutamine and an amino acid solution on post-thawed ram sperm parameters, lipid peroxidation and anti-oxidant activities. Small Rumin Res, 81, 13-17, 2009.
37. Michael A, Alexopoulos C, Pontiki E, Hadjipavlou-Litina D, Saratsis P, Boscos C: Efect of antioxidant supplementation on semen quality and reactive oxygen species of frozen-thawed canine spermatozoa. Theriogenology, 68, 204-212, 2007.
38. Fraser L, A. Dziekońska A, Strzeżek R, Strzeżek J: Dialysis of boar semen prior to freezing-thawing: Its efects on post-thaw sperm characteristics. Theriogenology, 67, 994-1003, 2007.
39. Ciftci H, Verit A, Savas M, Yeni E, Erel O: Efects of N-acetylcysteine on semen parameters and oxidative/antioxidant status. Urology, 74 (1): 205-207, 2009.
40. Marco-Jimenez F, Puchades S, Gadea J, Vicente JS, Viudes-de- Castro MP: Efect of semen collection method on pre- and post-thaw guirra ram spermatozoa. Theriogenolog y, 64, 1756-1765, 2005.
41. DAlessandro AG, Martemucci G: Evaluation of seasonal variations of semen freezability in leccese ram. Anim Reprod Sci, 79, 93-102, 2003.
42. Arı UÇ, Kulaksız R, Yıldız S, Öztürkler Y, Lehimcioğlu NC: Efect of N-acetylcysteine (NAC) on post-thaw semen quality of Tushin rams: Higher doses of NAC may be toxic. (Abstract) VII. National Reproduction and Artificial Insemination Science Congress, Book of Congress, p.148-149, Kars/TURKEY, 2013.

APA	YILDIZ S, OZTÜRKLER Y, ARI U, LEHİMCİOĞLU N, ATAKİŞİ E, KULAKSIZ R (2015). The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen. , 81 - 86.
Chicago	YILDIZ Savaş,OZTÜRKLER YAVUZ,ARI Umut Çağın,LEHİMCİOĞLU NECDET CANKAT,ATAKİŞİ Emine,KULAKSIZ Recai The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen. (2015): 81 - 86.
MLA	YILDIZ Savaş,OZTÜRKLER YAVUZ,ARI Umut Çağın,LEHİMCİOĞLU NECDET CANKAT,ATAKİŞİ Emine,KULAKSIZ Recai The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen. , 2015, ss.81 - 86.
AMA	YILDIZ S,OZTÜRKLER Y,ARI U,LEHİMCİOĞLU N,ATAKİŞİ E,KULAKSIZ R The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen. . 2015; 81 - 86.
Vancouver	YILDIZ S,OZTÜRKLER Y,ARI U,LEHİMCİOĞLU N,ATAKİŞİ E,KULAKSIZ R The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen. . 2015; 81 - 86.
IEEE	YILDIZ S,OZTÜRKLER Y,ARI U,LEHİMCİOĞLU N,ATAKİŞİ E,KULAKSIZ R "The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen." , ss.81 - 86, 2015.
ISNAD	YILDIZ, Savaş vd. "The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen". (2015), 81-86.

APA	YILDIZ S, OZTÜRKLER Y, ARI U, LEHİMCİOĞLU N, ATAKİŞİ E, KULAKSIZ R (2015). The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(1), 81 - 86.
Chicago	YILDIZ Savaş,OZTÜRKLER YAVUZ,ARI Umut Çağın,LEHİMCİOĞLU NECDET CANKAT,ATAKİŞİ Emine,KULAKSIZ Recai The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21, no.1 (2015): 81 - 86.
MLA	YILDIZ Savaş,OZTÜRKLER YAVUZ,ARI Umut Çağın,LEHİMCİOĞLU NECDET CANKAT,ATAKİŞİ Emine,KULAKSIZ Recai The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.21, no.1, 2015, ss.81 - 86.
AMA	YILDIZ S,OZTÜRKLER Y,ARI U,LEHİMCİOĞLU N,ATAKİŞİ E,KULAKSIZ R The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(1): 81 - 86.
Vancouver	YILDIZ S,OZTÜRKLER Y,ARI U,LEHİMCİOĞLU N,ATAKİŞİ E,KULAKSIZ R The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(1): 81 - 86.
IEEE	YILDIZ S,OZTÜRKLER Y,ARI U,LEHİMCİOĞLU N,ATAKİŞİ E,KULAKSIZ R "The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21, ss.81 - 86, 2015.
ISNAD	YILDIZ, Savaş vd. "The effects of L-Ergothioneine, N-acetylcystein and cystein on freezing of ram semen". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21/1 (2015), 81-86.