Aim: The aim of this study was to evaluate the levels of knowledge of pre-school teachers working in the province center of Isparta related with basic first-aid practices and some factors which affected these levels of knowledge. Material and Methods: In this cross-sectional, analytic study, 110 pre- school teachers working in the province center of Isparta constituted the population. A questionnaire questioning sociodemographic pro- perties and the level of knowledge related with first-aid practices was applied under supervision. The level of knowledge was evaluated on a 20-point scale. In the analyses, Kruskal-Wallis and Mann-Whitney U tests and Spearman s rank correlation were used. The study was approved by the Ethical Committee for Clinical Studies of Süleyman Demirel University School of Medicine (registration number: 105). Results: The mean score of first-aid knowledge of the pre-school teachers was found to be 11.9±2.9. The least known issues included washing the wound by soap and water after a dog bite, information related with the necessity of immobilization of a child who has fallen from a high level and the phone number of National Poison Infor- mation Center (16.4%, 20.9% and 22.7%, respectively). The scores of the subjects whose knowledge of first-aid was evaluated to be well were higher compared to the subjects whose knowledge of first-aid was evaluated to be moderate (p=0.009) and poor (p=0.001). It was fo- und that first-aid scores did not show significant difference in terms of age, working period, having received first-aid training and having faced with a condition requiring first-aid previously (p>0.05, for all comparisons). Conclusions: It was found that pre-school teachers had insufficient first-aid knowledge. Since the first-aid knowledge scores of the sub- jects who reported that they received first-aid training before did not show significant difference, it was thought that the quality of training was as important as receiving training. (Türk Ped Arş 2014; 49: 238-46)