A novel polymerase chain reaction to detect brucella canis in dogs

Aras, Zeki; AYDIN, Ibrahim; taspinar, mehmet

A novel polymerase chain reaction to detect brucella canis in dogs

Zeki ARAS, (Aksaray Üniversitesi Veteriner Fakültesi, Mikrobiyoloji Bölümü, Aksaray, Türkiye)

Mehmet TAŞPINAR, (Yüzüncü Yıl Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Van, Türkiye)

İbrahim AYDIN (Selçuk Üniversitesi, Veteriner Fakültesi, Doğum ve Jinekoloji Anabilim Dalı, Konya, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

2 0

Yıl: 2015 Cilt: 21 Sayı: 2 Sayfa Aralığı: 169 - 172 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

A novel polymerase chain reaction to detect brucella canis in dogs

Öz:

Bu çalışmada, köpeklerin vajinal sıvap örneklerinden Brucella canis (B. canis)in direk teşhisi amacıyla spesifik polimeraz zincir reaksiyonumetodu standardize edildi ve değerlendirildi. Brucella spp.in 16S-23S inter-space bölgesine ve B. canisin BMEI1426-BMEI1427 genbölgesinde ki 351 bplik delesyon alanına özgü primer çiftleri seçildi. polimeraz zincir reaksiyonu yönteminin değerlendirilmesindetoplam 21 referans ve saha suşu ile 35 vajinal sıvap örneği kullanıldı. Sadece 214 bplik amplifiye polimeraz zincir reaksiyonu ürünününgörüntülenmesi B. canis için pozitif olarak kabul edildi. Vajinal sıvap örneklerinden en az DNA tespit limiti yeni metot için 2.7 x 10 1CFU/g bakteri olarak bulundu. Bu yöntem bakteriyel izolasyon metoduna pratik bir alternatif olabilir. Yeni polimeraz zincir reaksiyonutesti B. canisin klinik veya saha örneklerinden 24 saat sürede ve tek adımda hızlı ve basit tespitini sağlamaktadır.

Anahtar Kelime:

Konular: Veterinerlik

Köpeklerde brucella canis in teşhisi amacıyla yeni polimeraz zincir reaksiyonu metodunun geliştirilmesi

Öz:

In this study, the specific polymerase chain reaction has been standardized and evaluated for the direct diagnosis of Brucella canis invaginal swab samples from dogs. The specific primer sets are directed to the 16S-23S rRNA inter-space region of Brucella spp. and thedeletion of 351 bp in BMEI1426-BMEI1427 in B. canis. A total of 21 references and field strains and 35 vaginal swab samples were usedfor the evaluation of the polymerase chain reaction. It found that polymerase chain reaction is positive for B. canis DNA indicated byonly ampliﬁcation of 214 bp product. It detected at least 2.7 x 10 1 CFU/g of bacteria diluted in vaginal swab samples indicates that thepolymerase chain reaction can be used as a practical alternative for bacterial isolation. The novel polymerase chain reaction provides asimple and rapid for the detection of B. canis in clinical and field samples in one step and in short time about 24 h.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Aras Z, taspinar m, AYDIN I (2015). A novel polymerase chain reaction to detect brucella canis in dogs. , 169 - 172.
Chicago	Aras Zeki,taspinar mehmet,AYDIN Ibrahim A novel polymerase chain reaction to detect brucella canis in dogs. (2015): 169 - 172.
MLA	Aras Zeki,taspinar mehmet,AYDIN Ibrahim A novel polymerase chain reaction to detect brucella canis in dogs. , 2015, ss.169 - 172.
AMA	Aras Z,taspinar m,AYDIN I A novel polymerase chain reaction to detect brucella canis in dogs. . 2015; 169 - 172.
Vancouver	Aras Z,taspinar m,AYDIN I A novel polymerase chain reaction to detect brucella canis in dogs. . 2015; 169 - 172.
IEEE	Aras Z,taspinar m,AYDIN I "A novel polymerase chain reaction to detect brucella canis in dogs." , ss.169 - 172, 2015.
ISNAD	Aras, Zeki vd. "A novel polymerase chain reaction to detect brucella canis in dogs". (2015), 169-172.

APA	Aras Z, taspinar m, AYDIN I (2015). A novel polymerase chain reaction to detect brucella canis in dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(2), 169 - 172.
Chicago	Aras Zeki,taspinar mehmet,AYDIN Ibrahim A novel polymerase chain reaction to detect brucella canis in dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21, no.2 (2015): 169 - 172.
MLA	Aras Zeki,taspinar mehmet,AYDIN Ibrahim A novel polymerase chain reaction to detect brucella canis in dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.21, no.2, 2015, ss.169 - 172.
AMA	Aras Z,taspinar m,AYDIN I A novel polymerase chain reaction to detect brucella canis in dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(2): 169 - 172.
Vancouver	Aras Z,taspinar m,AYDIN I A novel polymerase chain reaction to detect brucella canis in dogs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(2): 169 - 172.
IEEE	Aras Z,taspinar m,AYDIN I "A novel polymerase chain reaction to detect brucella canis in dogs." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21, ss.169 - 172, 2015.
ISNAD	Aras, Zeki vd. "A novel polymerase chain reaction to detect brucella canis in dogs". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21/2 (2015), 169-172.