Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits

ÇİNKILIÇ, Nilüfer; BAĞDAŞ, Deniz; WEHREND, Axel; VATAN, Özgür; ÖZALP, Gözde Rabia

Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits

Özgür VATAN, (Uludağ Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Bursa, Türkiye)

Deniz BAĞDAŞ, (Uludağ Üniversitesi, Veteriner Fakültesi Araştırma Uygulama Merkezi, Bursa, Türkiye)

Nilüfer ÇİNKILIÇ, (Uludağ Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Bursa, Türkiye)

Axel WEHREND, (Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Justus Liebig University, 35392, Giessen, Germany)

Gözde Rabia ÖZALP (Uludağ Üniversitesi, Veteriner Fakültesi, Doğum ve Jinekoloji Anabilim Dalı, Bursa, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

2 0

Yıl: 2015 Cilt: 21 Sayı: 2 Sayfa Aralığı: 241 - 246 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits

Öz:

Aglepriston, veteriner hekimlikte gebeliklerin sonlandırılmasında kullanılan bir antiprogestindir. Aglepristonun yan etkileri ileilgili bilgiler sınırlıdır. Çalışmamızın amacı; orta gebelikte gebeliği sonlandırılan tavşanlarda aglepristonun potansiyel sitotoksik vegenotoksik etkilerinin araştırılmasıdır. Çalışmada 15 Yeni Zelanda beyaz tavşanı kullanılmıştır ve gebe tavşanlar rastgele üç gruba ayrıldı.I. Grup (n=5) kontrol grubu olarak tuz çözeltisi ile muamele edildi. II. Grup (n=5) gebeliğin 15. gününde aglepriston (10 mg/kg) enjekteedilen III. Grup (n=5) ise gebeliğin 15. ve 16. günlerinde aglepristone enjekte edilen gruptu. Tavşanlar son enjeksiyonlardan 24 saatsonra giyotin ile sakrifiye edilerek hızlı bir şekilde kemik iliği ve kan örnekleri alındı. Sitotoksik ve genotoksik potansiyel mikroçekirdekve komet yöntemleri ile araştırıldı. Tek aglepriston uygulaması ile gebeliği sonlandırılan tavşanlarda mikroçekirdek testi ile herhangibir sitotoksik ve genotoksik etki belirlenemedi. Bunun tersine iki aglepriston uygulanan tavşanların kemik iliği hücrelerinde yükseksitotoksik ve genotoksik etki belirlendi. Komet yönteminde kullanılan kan örneklerinde gruplar arasında bir fark belirlenemedi. Kemikiliği hücrelerinin kullanıldığı komet yönteminde tek enjeksiyon grubunda herhangi bir DNA hasarı belirlenmemesine rağmen çiftenjeksiyon grubunda DNA hasarının arttığı belirlendi.

Anahtar Kelime:

Konular: Veterinerlik

Tavşanlarda orta dönem gebelikleri sonlandırılmasında kullanılan bir progesteron antagonisti olan aglepriston un genotoksik ve sitotoksik etkileri

Öz:

Aglepristone is an antiprogestin using for pregnancy termination in veterinary medicine. The information about side effects ofaglepristone is limited. The aim of the study was to investigate cytotoxicity and genotoxicity of aglepristone in mid-gestation pregnancytermination in rabbits. Fifteen New Zealand White rabbits were used and pregnant does were randomly divided into three groups.Group I (n=5) was treated with saline as the control. The does in group II (n=5) and group III (n=5) were treated with aglepristone (10mg/kg) on 15 th day and 15 th-16 th days of pregnancy, respectively. The rabbits were sacrificed by guillotine 24 h after last treatment.Bone marrow and blood samples were immediately collected. Cytotoxic and genotoxic potential were tested by micronucleus andComet assays. No genotoxicity and cytotoxicity were found in micronucleus test with single aglepristone administration. In contrast,two consecutive treatments of aglepristone showed high genotoxic and cytotoxic efects on bone marrow in animals. While cometassay of blood samples did not show any significant diference between groups; the results from comet assay of bone marrow cellsshowed the single injection of aglepristone did not induce any DNA damage but two injections group increased the DNA damage.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	VATAN Ö, BAĞDAŞ D, ÇİNKILIÇ N, WEHREND A, ÖZALP G (2015). Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits. , 241 - 246.
Chicago	VATAN Özgür,BAĞDAŞ Deniz,ÇİNKILIÇ Nilüfer,WEHREND Axel,ÖZALP Gözde Rabia Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits. (2015): 241 - 246.
MLA	VATAN Özgür,BAĞDAŞ Deniz,ÇİNKILIÇ Nilüfer,WEHREND Axel,ÖZALP Gözde Rabia Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits. , 2015, ss.241 - 246.
AMA	VATAN Ö,BAĞDAŞ D,ÇİNKILIÇ N,WEHREND A,ÖZALP G Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits. . 2015; 241 - 246.
Vancouver	VATAN Ö,BAĞDAŞ D,ÇİNKILIÇ N,WEHREND A,ÖZALP G Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits. . 2015; 241 - 246.
IEEE	VATAN Ö,BAĞDAŞ D,ÇİNKILIÇ N,WEHREND A,ÖZALP G "Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits." , ss.241 - 246, 2015.
ISNAD	VATAN, Özgür vd. "Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits". (2015), 241-246.

APA	VATAN Ö, BAĞDAŞ D, ÇİNKILIÇ N, WEHREND A, ÖZALP G (2015). Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(2), 241 - 246.
Chicago	VATAN Özgür,BAĞDAŞ Deniz,ÇİNKILIÇ Nilüfer,WEHREND Axel,ÖZALP Gözde Rabia Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21, no.2 (2015): 241 - 246.
MLA	VATAN Özgür,BAĞDAŞ Deniz,ÇİNKILIÇ Nilüfer,WEHREND Axel,ÖZALP Gözde Rabia Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.21, no.2, 2015, ss.241 - 246.
AMA	VATAN Ö,BAĞDAŞ D,ÇİNKILIÇ N,WEHREND A,ÖZALP G Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(2): 241 - 246.
Vancouver	VATAN Ö,BAĞDAŞ D,ÇİNKILIÇ N,WEHREND A,ÖZALP G Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(2): 241 - 246.
IEEE	VATAN Ö,BAĞDAŞ D,ÇİNKILIÇ N,WEHREND A,ÖZALP G "Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21, ss.241 - 246, 2015.
ISNAD	VATAN, Özgür vd. "Genotoxic and cytotoxic effects of the aglepristone, A progesteron antagonist, in Mid-gestation pregnancy termination in rabbits". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21/2 (2015), 241-246.