Purpose: In this study, we investigated the attitudes of healthcare workers in terms of seasonal and pre- pandemic influenza vaccinations just after influenza A (H1N1) 2009 pandemic and evaluated the reasons for the unwillingness for influenza vaccinations. Material and methods: Among 2803 health care workers from a training hospital in Ankara, as their occupational and educational status differs, 570 were chosen by stratified random sampling method for survey. In order to identify the attitudes for seasonal and influenza A (H1N1) 2009 vaccine(s), a total of eight questions were asked to responders. Results were evaluated statistically using the chi-square test. Results: Of the respondents, 42.3 % intended to receive seasonal influenza vaccine while 39.6 % were for pandemic influenza A (H1N1) 2009. On the other hand, the rate of those who want to be vaccinated with both vaccines was 37.2 %.The most important reasons of the people who do not want to be vaccinated with seasonal vaccine were that they wanted to refrain from adverse effect profile (42.5 %) and prefer other protection methods (18.2 %) and also they do not believe the necessity of vaccine (17.5%). While refraining from adverse effect profile (52.3 %) was the number one reason, not sufficiently tested of vaccine (18.6 %) and preferring other protection methods (7.3 %) were second and third reason respectively of the people who do not want to be vaccinated with pandemic influenza A (H1N1) 2009 vaccine. Conclusions: For increasing influenza vaccination rates among health care workers, more education is needed.