Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury

ACAR, Mehmet Ali; ALTAN, Egemen; ERKOÇAK, ÖMER FARUK; Aydin, Bahattin Kerem; Ugras, Serdar

Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury

Bahattin Kerem AYDIN, (Selçuk Üniversitesi, Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, Konya, Türkiye)

Egemen ALTAN, (Selçuk Üniversitesi, Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, Konya, Türkiye)

Mehmet Ali ACAR, (Selçuk Üniversitesi, Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, Konya, Türkiye)

Ömer Faruk ERKOÇAK, (Selçuk Üniversitesi, Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, Konya, Türkiye)

NEVZAT SERDAR UĞRAŞ (Sağlık Bakanlığı, Konya Eğitim ve Araştırma Hastanesi, Patoloji Bölümü, Konya, Türkiye)

Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.)

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Yıl: 2015 Cilt: 26 Sayı: 1 Sayfa Aralığı: 31 - 37 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury

Öz:

Amaç: Bu in vivo çalışmada yeni geliştirilen topikal birhemostatik ajan olan Ankaferd Kanama Durdurucu ®nun(ABS) sıçan Aşil tendon yaralanma modeli kullanılaraktendon iyileşmesi üzerine etkileri araştırıldı.Gereç ve yöntemler: Çalışmada 12 erkek Wistar-Albinosıçan kullanıldı. Sıçanların sağ bacakları çalışma grubu,sol bacakları kontrol grubu olarak belirlendi. Anestezialtında iki taraflı Aşil tendon yaralanmaları oluşturulduve cerrahi olarak tamir edildi. Cerrahi sahalara sağ tarafiçin 1 mL ABS, sol taraf için ise 1 mL serum fizyolojiksolüsyonu uygulandı. Hayvanlara ameliyat sonrası21. günde ötenazi yapıldı ve ekstremitelerden tendonörnekleri alındı. Histolojik analizler yarı kantitatiftendon skorlama sistemine (Bonar Sınıflaması) göreyapıldı.Bulgular: Toplam tendon iyileşmesi skorları çalışmagrubunda kontrol grubuna göre anlamlı şekilde kötüydü.Tenosit morfolojisi, esas madde boyama derecesi vevaskülarite açısından gruplar arasında anlamlı farklılıkolmasa da kollajen morfolojisi skorları çalışma grubundaanlamlı şekilde kötüydü.Sonuç: Çalışmamızda, ABS uygulaması sıçanlarda tendoniyileşmesi üzerinde histolojik olarak olumsuz etkiye sahipti.Öte yandan, bu sonuçları desteklemek için ileri biyomekanikve immünohistokimyasal çalışmalar gereklidir.

Anahtar Kelime:

Konular: Ortopedi

Ankaferd Kanama Durdurucu ® nun tendon iyileşmesi üzerine etkisi: Sıçan Aşil tendon yaralanma modelinde deneysel çalışma

Öz:

Objectives: This in vivo study aims to investigate the effectsof Ankaferd Blood Stopper ® (ABS) which is a recentlydeveloped topical hemostatic agent on tendon healing using arat Achilles tendon injury model.Materials and methods: Twelve male Wistar-Albino ratswere used in the study. Right legs of the rats were assignedas the study group, and left legs were assigned as thecontrol group. Under anesthesia, bilateral Achilles tendoninjuries were created and repaired surgically. To the surgicalsites, 1 mL of ABS for the right side and 1 mL of serumphysiologic solution for the left side were applied. Animalswere euthanized on the 21 st postoperative day, and tendonsamples were collected from the extremities. Histologicalanalyses were performed according to the semiquantitativetendon scoring system (Bonar Scale).Results: Total tendon healing scores were significantlypoorer in the study group compared to the control group.Although there were no significant differences between thegroups in terms of tenocyte morphology, degree of groundsubstance staining, and vascularity, the collagen morphologyscores were significantly poorer in the study group.Conclusion: In our study, application of ABS hadhistologically negative effect on tendon healing in rats.However, further biomechanical and immune histochemicalstudies are required to support these results.

Anahtar Kelime:

Konular: Ortopedi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Aydin B, ALTAN E, ACAR M, ERKOÇAK Ö, Ugras S (2015). Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury. , 31 - 37.
Chicago	Aydin Bahattin Kerem,ALTAN Egemen,ACAR Mehmet Ali,ERKOÇAK ÖMER FARUK,Ugras Serdar Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury. (2015): 31 - 37.
MLA	Aydin Bahattin Kerem,ALTAN Egemen,ACAR Mehmet Ali,ERKOÇAK ÖMER FARUK,Ugras Serdar Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury. , 2015, ss.31 - 37.
AMA	Aydin B,ALTAN E,ACAR M,ERKOÇAK Ö,Ugras S Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury. . 2015; 31 - 37.
Vancouver	Aydin B,ALTAN E,ACAR M,ERKOÇAK Ö,Ugras S Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury. . 2015; 31 - 37.
IEEE	Aydin B,ALTAN E,ACAR M,ERKOÇAK Ö,Ugras S "Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury." , ss.31 - 37, 2015.
ISNAD	Aydin, Bahattin Kerem vd. "Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury". (2015), 31-37.

APA	Aydin B, ALTAN E, ACAR M, ERKOÇAK Ö, Ugras S (2015). Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), 26(1), 31 - 37.
Chicago	Aydin Bahattin Kerem,ALTAN Egemen,ACAR Mehmet Ali,ERKOÇAK ÖMER FARUK,Ugras Serdar Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.) 26, no.1 (2015): 31 - 37.
MLA	Aydin Bahattin Kerem,ALTAN Egemen,ACAR Mehmet Ali,ERKOÇAK ÖMER FARUK,Ugras Serdar Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), vol.26, no.1, 2015, ss.31 - 37.
AMA	Aydin B,ALTAN E,ACAR M,ERKOÇAK Ö,Ugras S Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.). 2015; 26(1): 31 - 37.
Vancouver	Aydin B,ALTAN E,ACAR M,ERKOÇAK Ö,Ugras S Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.). 2015; 26(1): 31 - 37.
IEEE	Aydin B,ALTAN E,ACAR M,ERKOÇAK Ö,Ugras S "Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury." Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), 26, ss.31 - 37, 2015.
ISNAD	Aydin, Bahattin Kerem vd. "Effect of Ankaferd blood stopper ® on tendon healing: An experimental study in a rat model of Achilles tendon injury". Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.) 26/1 (2015), 31-37.