Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode

Özlem ÇOLAK, (Gazi Üniversitesi, Fen Bilimleri Enstitüsü, Kimya Anabilim Dalı, Ankara, Türkiye)
Fatma ARSLAN (Gazi Üniversitesi, Fen Fakültesi, Kimya Bölümü, Ankara, Türkiye)
Turkish Journal of Chemistry
2 0
Yıl: 2015 Cilt: 39 Sayı: 1 Sayfa Aralığı: 84 - 95 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode

Öz:
Abstract: A novel amperometric ethanol biosensor with alcohol dehydrogenase (ADH) and nicotinamide adenine dinucleotide (NAD^+) on polypyrrole--polyvinylsulfonate (PPy-PVS) film was accomplished. Meldola's blue (MB) was used as a mediator; it can facilitate the electron transfer that involves a chemical interaction between the electrode surface and NADH. Determination of ethanol was carried out by the oxidation of MB at --0.072 V vs. Ag/AgCl. The effects of pH and temperature were investigated and optimum parameters were found to be 7.0 and 40 °C, respectively. There are 2 linear parts at 1.0 mu M--10.0 mu M (R^2 = 0.993) and 0.01 mM--0.1 mM (R^2 = 0.996). The storage stability and operational stability of the enzyme electrode were also studied. The results showed that 95.7% of the response current was retained after 17 activity assays. The ethanol biosensor gave perfect reproducible finding with 4.6% relative standard deviation. The prepared biosensor retained 55.8% of initial activity after 24 days when stored in 0.1 M phosphate buffer solution at 4 °C. Effects of the several possible interfering substances such as ascorbic acid, citric acid, and methanol on the ethanol biosensor were investigated and the developed biosensor was tested in alcoholic beverages.
Anahtar Kelime:

Konular: Mühendislik, Kimya
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Santos, A. S.; Freire, R. S.; Kubota, L. T. J. Electroanal. Chem. 2003, 547, 135–142.
  • Santos, A. S.; Pereira, A. C.; Duran, N.; Kubota, L. T. Electrochim. Acta 2006, 52, 215–220.
  • Koyuncu, D.; Erden, P. E.; Pekyardımcı, S; Kılı ̧c, E. Anal. Lett. 2007, 40, 1904–1922.
  • Niculescu, M.; Erichsen, T.; Sukharev, V.; Kerenyi, Z.; Csoregi, E.; Schuhmann, W. Anal. Chim. Acta 2002, 463, 39–51.
  • Kochana, J.; Adamski, J. Cent. Eur. J. Chem. 2012, 10, 224–231.
  • Azevedo, A. M.; Prazeres, D. M. F.; Cabral, J. M. S.; Fonseca, L. P. Biosens. Bioelectron. 2005, 21, 235–247.
  • Alpat, S; Telefoncu, A. Sensors 2010, 10, 748–764.
  • Wang, M. L.; Choong, Y. M.; Su, N. W.; Lee, M. H. J. Food Drug Anal. 2003, 11, 133–140.
  • Apers, S.; Meenen, E.; Pieters, L.; Vlietinck, A. J. Pharmaceut. Biomed. 2003, 33, 529–537.
  • Li, W. J.; Wei, X. G.; Gong, W.; Ouyang, F. Chinese J. Chromatogr. 2000, 8, 170–172.
  • Harmon, B. J.; Patterson, D. H.; Regnier, F. E. J. Chromatogr. A 1993, 657, 429–434.
  • Sanford, C. L.; Mantooth, B. A.; Jones, B. T. J. Chem. Educ. 2001, 78, 1221–1224.
  • Barzana, E.; Klibanov, A. M.; Karel, M. A. Anal. Biochem. 1989, 182, 109–115.
  • Lazarova, G.; Genova, L.; Kostov, V. Acta Biotechnologica 1987, 7, 97–99.
  • Mello, L. D.; Kubota, L. T. Food Chem. 2002, 77, 237–256.
  • Park, J. K.; Yee, H. J.; Lee, K. S.; Lee, W. Y.; Shin, M. C.; Kim, T. H.; Kim, S. R. Anal. Chim. Acta 1999, 390, 83–91.
  • Pereira, A. C.; Aguiar, M. R.; Kisner, A.; Macedo, D. V.; Kubota, L. T. Sensor. Actuat. B Chem. 2007, 124, 269–276.
  • Barlett, P. N.; Tebbutt, P.; Whitaker, R. G. Prog. React. Kinet. Mec. 1991, 16, 55–155.
  • Simon, B. P.; Fabregas, E. Biosens. Bioelectron. 2006, 22, 131–137.
  • Carlson, B. W.; Miller, L. J. Am. Chem. Soc. 1985, 107, 479–485.
  • Mano, N.; Kuhn, A. J. Electroanal. Chem. 2000, 498, 58-66.
  • Kulys, J.; Blitewski, U.; Schmid, R. D. Anal. Lett. 1991, 24, 181–189. Tobalina, F.; Pariente, F.; Hernandez, L.; Abruna, H. D.; Lorenzo, E. Anal. Chim. Acta, 1999, 395, 17–26.
  • Gonzales, M. I. A.; Saidman, S. B.; Lobo-Castanon, M. J.; Miranda-Ordieres, A. J.; Blanco, P. T. Anal. Chem. 2000, 72, 520–527.
  • Arslan, F.; Ustaba ̧s, S.; Arslan, H. Sensors 2011, 11, 8152–8163.
  • Alvarez, N. L. S.; Ortea, P. M.; Paneda, A. M.; Castanon, M. J. L.; Ordieres, A. J. M.; Blanco, P. T. J. Electroanal. Chem. 2001, 502, 109–117.
  • Curulli, A.; Carelli, I.; Trischitta, O.; Palleschi, G. Talanta 1997, 44, 1659–1669.
  • Hoshino, T.; Sekiguchi, S.; Muguruma, H. Bioelectrochemistry 2012, 84, 1–5.
  • Persson, B.; Gorton, L. J. Electroanal. Chem. 1990, 292, 115–138.
  • Antiochia, R.; Lavagnini, I. Anal. Lett. 2006, 39, 1643–1655.
  • Gerard, M.; Chaubey, A.; Malhotra, B. D. Biosens. Bioelectron. 2002, 17, 345–359.
  • Gade, V. K.; Shirale, D. J.; Gaikwad, P. D.; Savale, P. A.; Kakde, K. P.; Kharat, H. J.; Shirsat, M. D. Int. J. Polym. Mater. 2007, 56, 1051–1065.
  • Colak, O.; Ya ̧sar, A.; Cete, S.; Arslan, F. Artificial Cells, Nanomedicine, and Biotechnology, 2012, 40, 354–361.
  • Bankar, S. B.; Bule, M. V.; Singhal, R. S.; Ananthanarayan, L. Biotechnol. Adv. 2009, 27, 489–501.
  • Manesh, K. M.; Santhosh, P.; Gopalan, A.; Lee, K. P. Talanta 2008, 75, 1307–1314.
  • Lou, P.; Liu, Y.; Xie, G.; Xiong, X.; Deng, S.; Song, F. Forensic Sci. Int. 2008, 179, 192–198.
  • Hasabe, Y.; Wang, Y.; Fukuoka, K. J. Environ. Sci. 2011, 23, 1050–1056.
  • Wen, G.; Zhang, Y.; Shuang, S.; Dong, C.; Choi, M. M. F. Biosens. Bioelectron. 2007, 23, 121–129.
  • Hendji, A. N.; Bataillard, P.; Jaffrezic-Renault, N. Sensor. Actuat. B Chem. 1993, 15, 127–134.
  • Jiang, X.; Zhu, L.; Yang, D.; Mao, X.; Wu, Y. Electroanalysis 2009, 21, 1617–1623.
  • Cai, C-X.; Xue, K-H.; Zhou, Y-M.; Yang, H. Talanta 1997, 44, 339–347.
  • Gros, P.; Durliat, H.; Comtat, M. Electrochim. Acta 2000, 46, 643–650.
  • Chaubey, A.; Gerard, M.; Singhal, M.; Singh, V. S.; Malhotra, B. D. Electrochim. Acta 2000, 46, 723–729.
APA Çolak Ö, ARSLAN F (2015). Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode. , 84 - 95.
Chicago Çolak Özlem,ARSLAN Fatma Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode. (2015): 84 - 95.
MLA Çolak Özlem,ARSLAN Fatma Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode. , 2015, ss.84 - 95.
AMA Çolak Ö,ARSLAN F Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode. . 2015; 84 - 95.
Vancouver Çolak Ö,ARSLAN F Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode. . 2015; 84 - 95.
IEEE Çolak Ö,ARSLAN F "Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode." , ss.84 - 95, 2015.
ISNAD Çolak, Özlem - ARSLAN, Fatma. "Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode". (2015), 84-95.
APA Çolak Ö, ARSLAN F (2015). Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode. Turkish Journal of Chemistry, 39(1), 84 - 95.
Chicago Çolak Özlem,ARSLAN Fatma Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode. Turkish Journal of Chemistry 39, no.1 (2015): 84 - 95.
MLA Çolak Özlem,ARSLAN Fatma Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode. Turkish Journal of Chemistry, vol.39, no.1, 2015, ss.84 - 95.
AMA Çolak Ö,ARSLAN F Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode. Turkish Journal of Chemistry. 2015; 39(1): 84 - 95.
Vancouver Çolak Ö,ARSLAN F Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode. Turkish Journal of Chemistry. 2015; 39(1): 84 - 95.
IEEE Çolak Ö,ARSLAN F "Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode." Turkish Journal of Chemistry, 39, ss.84 - 95, 2015.
ISNAD Çolak, Özlem - ARSLAN, Fatma. "Amperometric biosensing of ethanol based on integration of alcohol dehydrogenase with a Pt/PPy--PVS/MB electrode". Turkish Journal of Chemistry 39/1 (2015), 84-95.
Sayfa Oluşturulma Tarihi
22-04-2024 21:3

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