Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity

Müslüm KUZU, (Ağrı İbrahim Çeçen Üniversitesi, Eczacılık Fakültesi, Ağrı, Türkiye)
Mehmet ÇİFTÇİ (Atatürk Üniversitesi, Erzurum, Türkiye)
Turkish Journal of Chemistry
0 0
Yıl: 2015 Cilt: 39 Sayı: 1 Sayfa Aralığı: 149 - 158 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity

Öz:
Abstract: NADPH-cytochrome P450 reductase was purified from Lake Van fish liver microsomes by primary and secondary DEAE-cellulose column chromatograph with 20.46 mu M/min/mg enzyme specific activities, 54.4% purification yield, and purification of 38-fold. The purity of the enzyme was established, and its monomer molecular weight was determined by SDS-polyacrylamide gel electrophoresis. SDS-PAGE results showed a single band and the molecular weight of NADPH-cytochrome P450 reductase was 70 kDa. In addition, optimum ionic strength, optimum pH, optimum temperature, and stable pH values were determined for the enzyme in the kinetic studies performed. K_M and V_{max} were determined for NADPH and cytochrome c. Effects of some metals ions, antibiotics, and some other drugs used in aquarium fisheries on the activity of the enzyme were investigated. IC50 values and K_i values of metals showing an inhibitory effect were calculated.
Anahtar Kelime:

Konular: Mühendislik, Kimya
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA KUZU M, Ciftci M (2015). Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity. , 149 - 158.
Chicago KUZU Müslüm,Ciftci Mehmet Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity. (2015): 149 - 158.
MLA KUZU Müslüm,Ciftci Mehmet Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity. , 2015, ss.149 - 158.
AMA KUZU M,Ciftci M Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity. . 2015; 149 - 158.
Vancouver KUZU M,Ciftci M Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity. . 2015; 149 - 158.
IEEE KUZU M,Ciftci M "Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity." , ss.149 - 158, 2015.
ISNAD KUZU, Müslüm - Ciftci, Mehmet. "Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity". (2015), 149-158.
APA KUZU M, Ciftci M (2015). Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity. Turkish Journal of Chemistry, 39(1), 149 - 158.
Chicago KUZU Müslüm,Ciftci Mehmet Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity. Turkish Journal of Chemistry 39, no.1 (2015): 149 - 158.
MLA KUZU Müslüm,Ciftci Mehmet Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity. Turkish Journal of Chemistry, vol.39, no.1, 2015, ss.149 - 158.
AMA KUZU M,Ciftci M Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity. Turkish Journal of Chemistry. 2015; 39(1): 149 - 158.
Vancouver KUZU M,Ciftci M Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity. Turkish Journal of Chemistry. 2015; 39(1): 149 - 158.
IEEE KUZU M,Ciftci M "Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity." Turkish Journal of Chemistry, 39, ss.149 - 158, 2015.
ISNAD KUZU, Müslüm - Ciftci, Mehmet. "Purification and characterization of NADPH-cytochrome P450 reductase from Lake Van fish liver microsomes and investigation of some chemical and metals' effects on the enzyme activity". Turkish Journal of Chemistry 39/1 (2015), 149-158.
Sayfa Oluşturulma Tarihi
22-04-2024 21:2

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