Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]

ER, Mehlika; SOYUDAL, Bahadır; BALCI, Faruk; ALPAY, Fazlı; Ardicli, Sena; DİNÇEL, DENİZ; ŞAMLI, HALE

Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]

Deniz DİNÇEL, (Uludağ Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, Uludağ, Türkiye)

Sena ARDIÇLI, (Uludağ Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, Uludağ, Türkiye)

Bahadır SOYUDAL, (Uludağ Üniversitesi, Veteriner Fakültesi, Zootekni Anabilim Dalı, Bursa, Türkiye)

Mehlika ER, (Uludağ Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, Bursa, Türkiye)

Fazlı ALPAY, (Uludağ Üniversitesi, Veteriner Fakültesi, Zootekni Anabilim Dalı, Bursa, Türkiye)

Hale ŞAMLI, (Uludağ Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, Uludağ, Türkiye)

Faruk BALCI (Uludağ Üniversitesi, Veteriner Fakültesi, Genetik Anabilim Dalı, Uludağ, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

10 2

Yıl: 2015 Cilt: 21 Sayı: 4 Sayfa Aralığı: 483 - 486 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]

Öz:

Ovulasyon oranı ve bir batındaki yavru sayısı gibi dölverim özellikleri fekondite genleri tarafından regüle edilmektedir. Bu çalışma Sakız ırkı koyunlarda, Booroola (FecB), Galway (FecXG), Inverdale (FecXI ) ve Calpastatin (CAST) genlerindeki DNA polimorfizmlerini belirlemek amacıyla yapılmıştır. Araştırılan gen veya aleller açısından toplam 71 koyun PCR-RFLP metoduyla genotiplendirilmiştir. Çalışmadan çıkan sonuçlar, Sakız koyunların FecB, FecXG ya da FecXI ye ait mutasyonları taşımadığını göstermiştir. Ancak CAST geni açısından AA, AB ve BB genotiplerinin sırasıyla 0.59, 0.36 ve 0.05 frekans gösterdiği tespit edilmiştir. CAST geni açısından çalışılan sürünün Hardy-Weinberg dengesine uyduğu görülmüştür (P>0.05). Sonuç olarak yeni keşfedilen mutasyonların geliştirilmesi gibi daha yoğun tarama çalışmaları gerekmektedir. Ek olarak Sakız koyunlarında CAST genindeki polimorfizmin varlığı ilk defa bu çalışmada bildirilmektedir.

Anahtar Kelime:

Konular: Biyoloji

Sakız Irkı Koyunlarda FecB, BMP15 ve CAST Genlerindeki Mutasyonların Analizi

Öz:

Fertility traits, such as the ovulation rate and the number of offspring at birth, are genetically regulated by fecundity genes. This study was performed to identify DNA polymorphisms in Booroola (FecB), Galway (FecXG), Inverdale (FecXI ) and Calpastatin (CAST) genes in Sakiz sheep. A total of 71 ewes were genotyped for gene or allelic polymorphisms in the genes listed above using the PCR-RFLP method. The results obtained from this study indicated that all of the Sakiz ewes sampled were non-carriers for FecB, FecXG or FecXI mutations. However, genotypic frequencies in the CAST gene were 0.59, 0.36 and 0.05 for AA, AB and BB, respectively. A significant deviation from Hardy-Weinberg equilibrium for the CAST gene was not observed in the investigated breed (P>0.05). As a result, more extensive screening is required as tests for newly discovered mutations are developed. Additionally, this study is the first to report a genetic polymorphism in the CAST gene in Sakiz sheep.

Anahtar Kelime:

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	DİNÇEL D, Ardicli S, SOYUDAL B, ER M, ALPAY F, ŞAMLI H, BALCI F (2015). Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]. , 483 - 486.
Chicago	DİNÇEL DENİZ,Ardicli Sena,SOYUDAL Bahadır,ER Mehlika,ALPAY Fazlı,ŞAMLI HALE,BALCI Faruk Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]. (2015): 483 - 486.
MLA	DİNÇEL DENİZ,Ardicli Sena,SOYUDAL Bahadır,ER Mehlika,ALPAY Fazlı,ŞAMLI HALE,BALCI Faruk Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]. , 2015, ss.483 - 486.
AMA	DİNÇEL D,Ardicli S,SOYUDAL B,ER M,ALPAY F,ŞAMLI H,BALCI F Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]. . 2015; 483 - 486.
Vancouver	DİNÇEL D,Ardicli S,SOYUDAL B,ER M,ALPAY F,ŞAMLI H,BALCI F Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]. . 2015; 483 - 486.
IEEE	DİNÇEL D,Ardicli S,SOYUDAL B,ER M,ALPAY F,ŞAMLI H,BALCI F "Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]." , ss.483 - 486, 2015.
ISNAD	DİNÇEL, DENİZ vd. "Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]". (2015), 483-486.

APA	DİNÇEL D, Ardicli S, SOYUDAL B, ER M, ALPAY F, ŞAMLI H, BALCI F (2015). Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(4), 483 - 486.
Chicago	DİNÇEL DENİZ,Ardicli Sena,SOYUDAL Bahadır,ER Mehlika,ALPAY Fazlı,ŞAMLI HALE,BALCI Faruk Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21, no.4 (2015): 483 - 486.
MLA	DİNÇEL DENİZ,Ardicli Sena,SOYUDAL Bahadır,ER Mehlika,ALPAY Fazlı,ŞAMLI HALE,BALCI Faruk Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.21, no.4, 2015, ss.483 - 486.
AMA	DİNÇEL D,Ardicli S,SOYUDAL B,ER M,ALPAY F,ŞAMLI H,BALCI F Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(4): 483 - 486.
Vancouver	DİNÇEL D,Ardicli S,SOYUDAL B,ER M,ALPAY F,ŞAMLI H,BALCI F Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(4): 483 - 486.
IEEE	DİNÇEL D,Ardicli S,SOYUDAL B,ER M,ALPAY F,ŞAMLI H,BALCI F "Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21, ss.483 - 486, 2015.
ISNAD	DİNÇEL, DENİZ vd. "Analysis of FecB, BMP15 and CAST Gene Mutations in Sakiz Sheep [1]". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21/4 (2015), 483-486.