Glucose-6-phosphate dehydrogenase (G6PD) is an enzyme included in the pentose phosphate pathway with a cellularprotective effect against oxidative stress. The most frequent clinical manifestations of G6PD deficiency are neonatal jaundice and acute hemolytic anemia, whichis usually triggered by an exogenous agent such as drugs, infections and certain nutrients. Some G6PD variantscause chronic hemolysis, leading to congenital non-spherocytic hemolytic anemia. The disorder is inherited by X-linkedtransmission. Homozygote females and males are the cases at risk for G6PD deficiency. A 26-month-old girl presented with hemolytic anemia after eating broad beans. She was diagnosed with G6PDdeficiency and interestingly the G6DP level was also decreased in her father. There was no history of hemolytic anemiain the father despite multiple exposure to broad beans. We aimed to underline the different types of clinical expressionsof G6PD deficiency in different genders by presenting this case report.