Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur

KORKUSUZ, PETEK; HESAR, Ramin Hashemi; EKŞİOĞLU, M. Fatih; TOGRAL, GURAY; ARIKAN, Ş. Murat

Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur

Güray TOĞRAL, (Dr. Abdurrahman Yurtaslan Ankara Onkoloji Eğitim ve Araştırma Hastanesi, Ortopedi ve Travmatoloji Anabilim Dalı, Ankara, Türkiye)

Ş. Murat ARIKAN, (Dr. Abdurrahman Yurtaslan Ankara Onkoloji Eğitim ve Araştırma Hastanesi, Ortopedi ve Travmatoloji Anabilim Dalı, Ankara, Türkiye)

Petek KORKUSUZ, (Hacettepe Üniversitesi, Tıp Fakültesi, Histolojİ ve Embriyoloji Anabilim Dalı, Ankara, Türkiye)

Ramin Hashemi HESAR, (Dr. Abdurrahman Yurtaslan Ankara Onkoloji Eğitim ve Araştırma Hastanesi, Ortopedi ve Travmatoloji Anabilim Dalı, Ankara, Türkiye)

M. Fatih EKŞİOĞLU (Dr. Abdurrahman Yurtaslan Ankara Onkoloji Eğitim ve Araştırma Hastanesi, Ortopedi ve Travmatoloji Anabilim Dalı, Ankara, Türkiye)

Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.)

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Yıl: 2015 Cilt: 26 Sayı: 3 Sayfa Aralığı: 137 - 144 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur

Öz:

Amaç: Bu çalışmada tadalafilin kırık iyileşmesini sildenafille eşit bir etkinlikte hızlandırıp hızlandırmadığı araştırıldı. Gereç ve yöntemler: Kırık iyileşmesi sıçan kapalı femur kırığı örneği kullanılarak incelendi. Kırk sekiz erkek Wistar albino sıçan (ort. yaş 13 hafta; dağılım 12-14 hafta) üç gruba ayrıldı: 16sına sildenafil (5 mg/kg/gün, oral), 16sına tadalafil (1 mg/kg/gün, oral) ve 16sına (kontrol grubu) her gün serum fizyolojik verildi. İki ve beş hafta sonra her gruptan sekiz sıçana ötanazi yapıldı ve kemik iyileşmesi radyolojik, histolojik ve histomorfometrik analizlerle değerlendirildi. Bulgular: İkinci haftanın sonunda tadalafil grubunun radyolojik skoru sildenafil grubundan yüksek ve her tadanafil hem sidenafil gruplarının skoru kontrol grubundan yüksekken, beşinci haftanın sonunda tüm grupların skorları benzerdi. Sildenafil ve tadalafil uygulanan gruplarda ikinci haftada kallus çapı kontrol grubuna göre daha küçük olarak saptanmış olsa da bu fark istatistiksel olarak anlamlı değildir. Beşinci haftadaki kallus çapı tadalafil grubunda kontrol grubuna göre anlamlı olarak küçüldü. Kontrol grubu ile karşılaştırıldığında, kallusun kıkırdak yüzdesi ikinci haftanın sonunda sildenafil grubunda daha düşük, beşinci haftanın sonunda hem tadalafil hem sildenafil grubunda daha düşüktü. Tadalafil ve sildenafil grupları arasında radyolojik skorlar, kallus çapı, kallusun kıkırdak ve kemik yüzdeleri ve köprüleşme skorları açısından ikinci ve beşinci haftanın sonunda farklılık yoktu. Sonuç: Bu çalışma; tadalafilin sildenafil ile benzer şekilde kemik doku oluşumunu artırarak kırık iyileşmesini hızlandırdığını göstermiştir.

Anahtar Kelime:

Konular: Ortopedi

Bir fosfodiesteraz 5 inhibitörü olan tadalafilin sıçan femurunda kırık iyileşmesi üzerindeki olumlu etkisi

Öz:

Objectives: This study aims to investigate whether tadalafil accelerates fracture healing with an efficiency equal to that of sildenafil. Materials and methods: Fracture healing was studied using a rat closed femur fracture model. Forty-eight male Wistar albino rats (mean age 13 weeks; range 12 to 14 weeks) were divided into three groups: 16 received sildenafil (5 mg/kg/day per oral), 16 received tadalafil (1 mg/kg/day per oral), and 16 (control group) received saline daily. After two and five weeks, eight rats from each group were euthanized and bone healing was evaluated using radiographic, histologic, and histomorphometric analyses. Results: At second week, radiologic score of tadalafil group was higher than the sildenafil treated groups and the sildenafil and tadalafil groups both had higher radiographic scores than the control group, all groups had similar scores at fifth week. The sildenafil and tadalafil treated group exhibited smaller callus diameter at the second week comparing to control group but the difference was not statistically significant, while callus diameter was significantly smaller in tadalafil group when compared to the control group in fifth week. Compared with the control group, cartilage percentage of the callus was lower in the sildenafil group at two weeks and lower in both the tadalafil and sildenafil groups at five weeks. There were no differences between the tadalafil and sildenafil groups regarding the radiographic scores, callus size, cartilage and osseous callus percentage, and the qualitative bridging scores at second or fifth week. Conclusion: This study demonstrated that tadalafil accelerates fracture healing by enhancing osseous tissue formation similar to sildenafil.

Anahtar Kelime:

Konular: Ortopedi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	TOGRAL G, ARIKAN Ş, KORKUSUZ P, HESAR R, EKŞİOĞLU M (2015). Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur. , 137 - 144.
Chicago	TOGRAL GURAY,ARIKAN Ş. Murat,KORKUSUZ PETEK,HESAR Ramin Hashemi,EKŞİOĞLU M. Fatih Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur. (2015): 137 - 144.
MLA	TOGRAL GURAY,ARIKAN Ş. Murat,KORKUSUZ PETEK,HESAR Ramin Hashemi,EKŞİOĞLU M. Fatih Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur. , 2015, ss.137 - 144.
AMA	TOGRAL G,ARIKAN Ş,KORKUSUZ P,HESAR R,EKŞİOĞLU M Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur. . 2015; 137 - 144.
Vancouver	TOGRAL G,ARIKAN Ş,KORKUSUZ P,HESAR R,EKŞİOĞLU M Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur. . 2015; 137 - 144.
IEEE	TOGRAL G,ARIKAN Ş,KORKUSUZ P,HESAR R,EKŞİOĞLU M "Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur." , ss.137 - 144, 2015.
ISNAD	TOGRAL, GURAY vd. "Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur". (2015), 137-144.

APA	TOGRAL G, ARIKAN Ş, KORKUSUZ P, HESAR R, EKŞİOĞLU M (2015). Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), 26(3), 137 - 144.
Chicago	TOGRAL GURAY,ARIKAN Ş. Murat,KORKUSUZ PETEK,HESAR Ramin Hashemi,EKŞİOĞLU M. Fatih Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.) 26, no.3 (2015): 137 - 144.
MLA	TOGRAL GURAY,ARIKAN Ş. Murat,KORKUSUZ PETEK,HESAR Ramin Hashemi,EKŞİOĞLU M. Fatih Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), vol.26, no.3, 2015, ss.137 - 144.
AMA	TOGRAL G,ARIKAN Ş,KORKUSUZ P,HESAR R,EKŞİOĞLU M Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.). 2015; 26(3): 137 - 144.
Vancouver	TOGRAL G,ARIKAN Ş,KORKUSUZ P,HESAR R,EKŞİOĞLU M Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.). 2015; 26(3): 137 - 144.
IEEE	TOGRAL G,ARIKAN Ş,KORKUSUZ P,HESAR R,EKŞİOĞLU M "Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur." Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), 26, ss.137 - 144, 2015.
ISNAD	TOGRAL, GURAY vd. "Positive effect of tadalafil, a phosphodiesterase-5 inhibitor, on fracture healing in rat femur". Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.) 26/3 (2015), 137-144.