Yıl: 2015 Cilt: 32 Sayı: 4 Sayfa Aralığı: 311 - 316 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients

Öz:
Amaç: İnterlökin-18 (İL-18), İL-1 süper ailesine ait bir sitokin olup, bağışıklık sistemine ait olan ve olmayan çeşitli hücrelerden salınmaktadır. Yapılan çalışmalar, İL-18'in hem anti-kanser hem de kansere öncülük eden etkilere sahip olduğunu göstermiştir. Bu çalışmanın amacı, kronik lenfositik lösemili (KLL) ve kronik miyeloid lösemili (KML) Türk hastalarda İL-18 gen polimorfizmleri ilişkisini değerlendirmektir.Gereç ve Yöntemler: İL-18 polimorfizleri (rs61667799(G/T), rs5744227(C/G), rs5744228(A/G) ve rs187238(G/C)), 20 KLL ve 30 KML hasta ve 30 sağlıklı bireyde araştırılmıştır. Genotipleme, polimeraz zincir reaksiyonu ve DNA dizi analizi ile gerçekleştirilmiştir.Bulgular: İL-18 geninde, rs187238(G/C) polimorfizmi ile kronik lösemi arasında anlamlı bir ilişki belirlenmiştir. KML hastalarında kontrol grubuna göre, C allelinin daha yüksek olduğu bulunmuştur. Kontroller ile karşılaştırıldığında, GC heterozigot ve CC homozigot genotipleri KML hastalarında risk oluşturmaktadır. Ancak, C alleli sıklığı kontrollere göre KLL olgularında istatistiksel olarak anlamlı değildir. KLL hastaları ve kontrol grubunun homozigot CC genotipi arasında anlamlı farklılık vardır ve bunun sonucu olarak CC genotipi, KLL hastaları için risk taşımaktadır denilebilir. Verilerimize dayanarak, KLL ve KML hastalarında, İL-18 geninde rs61667799(G/T), rs5744227(C/G) ve rs5744228(A/G) polimorfizmleri arasında anlamlı bir ilişki yoktur.Sonuç: İL-18 geninin promotor bölgesindeki rs187238(G/C) polimorfizmi Türk popülasyonunda kronik lösemi ile ilişkilidir. Ancak, yapılan bu çalışma, hasta sayısının sınırlı olması nedeniyle, -137G/C polimorfizmi ve hastalar (KLL ve KML) arasındaki ilişkiyi gösteren bir ön çalışma niteliğindedir. Mevcut bulguları doğrulamak için, haplotip ve gen ifade düzeyi analizleri ile birleştirilmiş daha geniş çaplı çalışmalara ihtiyaç vardır
Anahtar Kelime:

Konular: Hematoloji

İnterlökin 18 Geninin Promotör Bölgesindeki -137G/C Polimorfizmi Türk Popülasyonunda Kronik Lenfositik ve Kronik Miyeloid Lösemi Riskini Arttırmaktadır

Öz:
Objective: Interleukin-18 (IL-18) is a cytokine that belongs to the IL-1 superfamily and is secreted by various immune and nonimmune cells. Evidence has shown that IL-18 has both anticancer and procancer effects. The aim of this study was to evaluate the relationship between IL-18 gene polymorphisms and susceptibility to chronic lymphocytic leukemias (CLL) and chronic myelogenous leukemias (CML) in Turkish patients.Materials and Methods: The frequencies of polymorphisms (rs61667799(G/T), rs5744227(C/G), rs5744228(A/G), and rs187238(G/C)) were studied in 20 CLL patients, 30 CML patients, and 30 healthy individuals. The genotyping was performed by polymerase chain reaction and DNA sequencing analysis.Results: Significant associations were detected between the IL-18 rs187238(G/C) polymorphism and chronic leukemia. A higher prevalence of the C allele was found in CML cases with respect to controls. The GC heterozygous and CC homozygous genotypes were associated with risk of CML when compared with controls. However, prevalence of the C allele was not significantly high in CLL cases with respect to controls. There was only a significant difference between the homozygous CC genotype of CLL patients and the control group; thus, it can be concluded that the CC genotype may be associated with the risk of CLL. Based on our data, there were no significant associations between the IL-18 rs61667799(G/T), rs5744227(C/G), or rs5744228(A/G) polymorphisms and CLL or CML.Conclusions: IL-18 gene promoter rs187238(G/C) polymorphism is associated with chronic leukemia in the Turkish population. However, due to the limited number of studied patients, these are preliminary results that show the association between -137G/C polymorphism and patients (CLL and CML). Further large-scale studies combined with haplotype and expression analysis are required to validate the current findings
Anahtar Kelime:

Konular: Hematoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA YALÇIN S, MUTLU P, ÇETİN T, Sarper M, Ozgur G, AVCU F (2015). The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients. , 311 - 316.
Chicago YALÇIN Serap,MUTLU Pelin,ÇETİN Türker,Sarper Meral,Ozgur Gokhan,AVCU Ferit The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients. (2015): 311 - 316.
MLA YALÇIN Serap,MUTLU Pelin,ÇETİN Türker,Sarper Meral,Ozgur Gokhan,AVCU Ferit The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients. , 2015, ss.311 - 316.
AMA YALÇIN S,MUTLU P,ÇETİN T,Sarper M,Ozgur G,AVCU F The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients. . 2015; 311 - 316.
Vancouver YALÇIN S,MUTLU P,ÇETİN T,Sarper M,Ozgur G,AVCU F The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients. . 2015; 311 - 316.
IEEE YALÇIN S,MUTLU P,ÇETİN T,Sarper M,Ozgur G,AVCU F "The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients." , ss.311 - 316, 2015.
ISNAD YALÇIN, Serap vd. "The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients". (2015), 311-316.
APA YALÇIN S, MUTLU P, ÇETİN T, Sarper M, Ozgur G, AVCU F (2015). The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients. Turkish Journal of Hematology, 32(4), 311 - 316.
Chicago YALÇIN Serap,MUTLU Pelin,ÇETİN Türker,Sarper Meral,Ozgur Gokhan,AVCU Ferit The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients. Turkish Journal of Hematology 32, no.4 (2015): 311 - 316.
MLA YALÇIN Serap,MUTLU Pelin,ÇETİN Türker,Sarper Meral,Ozgur Gokhan,AVCU Ferit The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients. Turkish Journal of Hematology, vol.32, no.4, 2015, ss.311 - 316.
AMA YALÇIN S,MUTLU P,ÇETİN T,Sarper M,Ozgur G,AVCU F The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients. Turkish Journal of Hematology. 2015; 32(4): 311 - 316.
Vancouver YALÇIN S,MUTLU P,ÇETİN T,Sarper M,Ozgur G,AVCU F The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients. Turkish Journal of Hematology. 2015; 32(4): 311 - 316.
IEEE YALÇIN S,MUTLU P,ÇETİN T,Sarper M,Ozgur G,AVCU F "The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients." Turkish Journal of Hematology, 32, ss.311 - 316, 2015.
ISNAD YALÇIN, Serap vd. "The -137G/C Polymorphism in Interleukin-18 Gene Promoter Contributes to Chronic Lymphocytic and Chronic Myelogenous Leukemia Risk in Turkish Patients". Turkish Journal of Hematology 32/4 (2015), 311-316.