Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats
Yıl: 2016 Cilt: 22 Sayı: 1 Sayfa Aralığı: 7 - 11 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats
Öz: AMAÇ: Periferik sinir defektlerinin onarımda ven greftleri ile beraber hücre kültürleri kullanımı çalışmaları literatürde bulunmaktadır. Yağ dokusu kaynaklı kök hücrelerin periferik sinir iyileşmesini artırdığını gösteren çalışmalar literatürde bulunmaktadır. Bu nedenlerle periferik sinir defekti onarımlarında ven grefti ve yağ dokusu kaynaklı kök hücre kombine olarak kullanımı etkilerini değerlendirmek amacı ile sıçanlarda deneysel çalışma uyguladık.GEREÇ VE YÖNTEM: Bunun için yaklaşık 300 gr ağırlığında 30 adet Wistar albino cinsi sıçan üç çalışma grubuna ayrıldı. Birinci cerrahi basamakta tüm hayvanların sağ tibial sinirlerinde yaklaşık 8 mm sinir defekti oluşturuldu. Grup 1'de (10 hayvan) otojensinir grefti kullanıldı. Grup 2'de (10 hayvan) içi boş ven grefti ile, Grup 3'de (10 hayvan) ise ven grefti içine yağ dokusu kaynaklı kök hücre doldurularak onarım uygulandı. Bu işlemden üç ay sonra çalışmanın ikinci cerrahi basamağında hayvanlar sakrifiye edilerek onarım uygulanan sinir bölgesinden biyopsi alındı. Alınan örnekler ışık mikroskobu ile histopatolojik olarak değerlendirildi.BULGULAR: İstatistiksel olarak gruplar arasında miyelin ve akson çapı arasında anlamlı farklar yokken, akson sayısı açısından anlamlı farklar mevcuttu.TARTIŞMA: Sonuçlarımıza göre periferik sinir kayıplarının onarımında SVF ve ven grefti kombinasyonu uygulanabilir bir seçenektir.
Anahtar Kelime: Konular:
Yağ dokusundan elde edilmiş stromal vasküler fraksiyonla doldurulmuş ven greftleri ile periferik sinir kayıplarının onarımı: Deneysel çalışma
Öz: BACKGROUND: There are many studies in the literature combining vein grafts with cell cultures, muscle, tendon, and nerve tissues, but none of them could replace isolated nerve grafts for nerve repair in clinical settings. There are studies in the literature indicating that adipose tissue derived stromal vascular fraction (SVF) enhances peripheral nerve healing. Considering these facts, an experimental study was performed in rats based on combining SVF with vein grafts for peripheral nerve defect repairs.METHODS: The study included 30 rats which were divided in three groups. In the first stage, an 8 mm nerve defect was created in tibial nerve of each rat. In Group 1, the defect was reconstructed with nerve graft, in Group 2, the defect was reconstructed with vein graft, and in Group 3, the defect was reconstructed with vein graft filled with SVF solution. After 3 months, the second surgical stage was performed and nerve biopsies were taken. Tissue samples were observed histopathologically.RESULTS: There were no statistically meaningful difference between nerve grafts, vein grafts and adipose tissue derived SVF- vein grafts combination groups considering myelin diameter and axonal diameter. Axon count was statistically superior in the nerve graft and study groups when compared to empty vein groups.CONCLUSION: In conclusion, our results support the usage of stromal vascular fraction-vein graft combination for peripheral nerve defect repairs
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Diğer Erişim Türü: Erişime Açık
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| APA | ÖZKAN H, SİLİSTRELİ Ö, ERGÜR B, İRKÖREN S (2016). Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats. , 7 - 11. |
| Chicago | ÖZKAN Heval Selman,SİLİSTRELİ Özlem Karataş,ERGÜR Bekir,İRKÖREN Saime Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats. (2016): 7 - 11. |
| MLA | ÖZKAN Heval Selman,SİLİSTRELİ Özlem Karataş,ERGÜR Bekir,İRKÖREN Saime Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats. , 2016, ss.7 - 11. |
| AMA | ÖZKAN H,SİLİSTRELİ Ö,ERGÜR B,İRKÖREN S Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats. . 2016; 7 - 11. |
| Vancouver | ÖZKAN H,SİLİSTRELİ Ö,ERGÜR B,İRKÖREN S Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats. . 2016; 7 - 11. |
| IEEE | ÖZKAN H,SİLİSTRELİ Ö,ERGÜR B,İRKÖREN S "Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats." , ss.7 - 11, 2016. |
| ISNAD | ÖZKAN, Heval Selman vd. "Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats". (2016), 7-11. |
| APA | ÖZKAN H, SİLİSTRELİ Ö, ERGÜR B, İRKÖREN S (2016). Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats. Ulusal Travma ve Acil Cerrahi Dergisi, 22(1), 7 - 11. |
| Chicago | ÖZKAN Heval Selman,SİLİSTRELİ Özlem Karataş,ERGÜR Bekir,İRKÖREN Saime Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats. Ulusal Travma ve Acil Cerrahi Dergisi 22, no.1 (2016): 7 - 11. |
| MLA | ÖZKAN Heval Selman,SİLİSTRELİ Özlem Karataş,ERGÜR Bekir,İRKÖREN Saime Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats. Ulusal Travma ve Acil Cerrahi Dergisi, vol.22, no.1, 2016, ss.7 - 11. |
| AMA | ÖZKAN H,SİLİSTRELİ Ö,ERGÜR B,İRKÖREN S Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats. Ulusal Travma ve Acil Cerrahi Dergisi. 2016; 22(1): 7 - 11. |
| Vancouver | ÖZKAN H,SİLİSTRELİ Ö,ERGÜR B,İRKÖREN S Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats. Ulusal Travma ve Acil Cerrahi Dergisi. 2016; 22(1): 7 - 11. |
| IEEE | ÖZKAN H,SİLİSTRELİ Ö,ERGÜR B,İRKÖREN S "Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats." Ulusal Travma ve Acil Cerrahi Dergisi, 22, ss.7 - 11, 2016. |
| ISNAD | ÖZKAN, Heval Selman vd. "Repairing peripheral nerve defects by vein grafts filled with adipose tissue derived stromal vascular fraction: an experimental study in rats". Ulusal Travma ve Acil Cerrahi Dergisi 22/1 (2016), 7-11. |
