Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study

BEKLER, Halil; ÖZKUT, Afşar; KILINÇOĞLU, VOLKAN; özler, turhan; SERVET, Erkan

Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study

Erkan SERVET, (Medical Park Hastanesi, Ortopedi ve Travmatoloji Kliniği, Gaziantep, Türkiye)

Halil BEKLER, (Dr. Lütfi Kırdar Kartal Eğitim ve Araştırma Hastanesi, Ortopedi ve Travmatoloji Kliniği, İstanbul, Türkiye)

Volkan KILINÇOĞLU, (Bahçeşehir Üniversitesi, Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, İstanbul, Türkiye)

Turhan ÖZLER, (Yeditepe Üniversitesi, Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, İstanbul, Türkiye)

Afşar ÖZKUT (Göztepe Eğitim ve Araştırma Hastanesi, Ortopedi ve Travmatoloji Kliniği, İstanbul, Türkiye)

Acta Orthopaedica et Traumatologica Turcica

1 0

Yıl: 2016 Cilt: 50 Sayı: 2 Sayfa Aralığı: 234 - 241 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study

Öz:

Objective: Epineural scar formation is one of the most significant negative factors affecting surgical repair after peripheral nerve injury. The scar tissue mechanically hinders axonal regeneration and causes adhesions between nerves and surrounding tissues. A hemostatic agent Ankaferd Blood Stopper (ABS; İmmun Gıda İlaç Kozmetik San. ve Tic. Ltd. Şti., Istanbul, Turkey) has not been previously used. Decreasing the postoperative bleeding and adhesions between nerve and surrounding tissues will prevent the formation of scar tissue, as well as corresponding compressive neuropathy and/or deceleration of axonal regeneration. The purpose of this experimental study was to investigate the effects of bleeding on nerve healing and scar tissue after repair of peripheral nerve injuries.Methods: The right sciatic nerve of 30 Sprague-Dawley male rats (weighing 260-330 g) was cut 1.5 cm proximal to the trifurcation and repaired primarily with 8/0 sutures using epineural technique. The rats were then divided into 3 groups. Saline was applied in Group 1 (n=10), ABS in Group 2 (n=10), and heparin in Group 3 (n=10) for 5 minutes to the repair site and surrounding tissues. In each group, electrophysiological measurements were performed with electromyography (EMG) at postoperative week 12. Magnetic resonance diffusion tensor imaging was used at week 12. Macroscopical and histopathological evaluations were conducted after sacrificing the rats at week 24 with total excision of the repaired sciatic nerves and surrounding tissues.Results: The ABS and saline groups showed better healing than the heparin group. The ABS and saline groups were better in the histopathologic evaluations, but there was no statistically significant difference between the 2 groups.Conclusion: Statistically significant differences were not found between the 3 groups. Significant results may be obtained with larger studies

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Bibliyografik

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APA	SERVET E, BEKLER H, KILINÇOĞLU V, özler t, ÖZKUT A (2016). Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study. , 234 - 241.
Chicago	SERVET Erkan,BEKLER Halil,KILINÇOĞLU VOLKAN,özler turhan,ÖZKUT Afşar Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study. (2016): 234 - 241.
MLA	SERVET Erkan,BEKLER Halil,KILINÇOĞLU VOLKAN,özler turhan,ÖZKUT Afşar Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study. , 2016, ss.234 - 241.
AMA	SERVET E,BEKLER H,KILINÇOĞLU V,özler t,ÖZKUT A Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study. . 2016; 234 - 241.
Vancouver	SERVET E,BEKLER H,KILINÇOĞLU V,özler t,ÖZKUT A Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study. . 2016; 234 - 241.
IEEE	SERVET E,BEKLER H,KILINÇOĞLU V,özler t,ÖZKUT A "Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study." , ss.234 - 241, 2016.
ISNAD	SERVET, Erkan vd. "Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study". (2016), 234-241.

APA	SERVET E, BEKLER H, KILINÇOĞLU V, özler t, ÖZKUT A (2016). Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study. Acta Orthopaedica et Traumatologica Turcica, 50(2), 234 - 241.
Chicago	SERVET Erkan,BEKLER Halil,KILINÇOĞLU VOLKAN,özler turhan,ÖZKUT Afşar Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study. Acta Orthopaedica et Traumatologica Turcica 50, no.2 (2016): 234 - 241.
MLA	SERVET Erkan,BEKLER Halil,KILINÇOĞLU VOLKAN,özler turhan,ÖZKUT Afşar Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study. Acta Orthopaedica et Traumatologica Turcica, vol.50, no.2, 2016, ss.234 - 241.
AMA	SERVET E,BEKLER H,KILINÇOĞLU V,özler t,ÖZKUT A Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study. Acta Orthopaedica et Traumatologica Turcica. 2016; 50(2): 234 - 241.
Vancouver	SERVET E,BEKLER H,KILINÇOĞLU V,özler t,ÖZKUT A Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study. Acta Orthopaedica et Traumatologica Turcica. 2016; 50(2): 234 - 241.
IEEE	SERVET E,BEKLER H,KILINÇOĞLU V,özler t,ÖZKUT A "Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study." Acta Orthopaedica et Traumatologica Turcica, 50, ss.234 - 241, 2016.
ISNAD	SERVET, Erkan vd. "Effect of bleeding on nerve regeneration and epineural scar formation in rat sciatic nerves: an experimental study". Acta Orthopaedica et Traumatologica Turcica 50/2 (2016), 234-241.