Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats

UÇAR, ÖMER; AKMAN, Orhan; ÖMÜR, Ali Doğan; özkaraca, mustafa; AKSU, Emrah Hicazi

Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats

Emrah Hicazi AKSU, (Atatürk Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Erzurum, Türkiye)

Orhan AKMAN, (Atatürk Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Erzurum, Türkiye)

Mustafa ÖZKARACA, (Atatürk Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Erzurum, Türkiye)

Ali Doğan ÖMÜR, (Atatürk Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Erzurum, Türkiye)

Ömer UÇAR (Atatürk Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Erzurum, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

7 1

Yıl: 2015 Cilt: 21 Sayı: 3 Sayfa Aralığı: 397 - 403 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats

Öz:

Bu çalışmanın amacı, maclura pomifera (MP) (sitotoksik, anti-tümöral, yangı önleyici, analjezik/ağrı giderici, ve antioksidan olarak bilinen) ekstraktının sisplatin (ROS (Reaktif oksijen türevleri) -oluşturucu yan etkili anti-tümöral ajan) uygulanan erkek ratların spermatolojik parametreleri üzerine etkisini incelemekti. Araştırmada 8 haftalık yaşta, 250-300 g canlı ağırlığa sahip 17 adet erkek Sprague Dawley ırkı rat kullanıldı. Üç deney grubu oluşturuldu; Grup 1 (n=5): kontrol grubu, hayvanlara intraperitonal (IP) serum fizyolojik enjeksiyonuyla birlikte, enjeksiyondan 5 gün öncesi ve sonrası boyunca oral gavaj yoluyla yine serum fizyolojik verildi. Grup 2 (n=6): Sisplatin (CP) grubu, hayvanlara bir kez IP 7 mg/kg CP enjeksiyonundan 5 gün öncesi ve sonrası boyunca oral yolla serum fizyolojik verildi. Grup 3 (n=6): MP + CP grubu, hayvanlara bir kez İP 7 mg/kg CP enjeksiyonuyla birlikte, enjeksiyondan 5 gün öncesi ve sonrası boyunca oral yolla günlük 500 mg/kg MP ekstraktı verildi. Deney sonunda ratlar dekapite edilerek testis ve kauda epididimis ağırlıkları ile epididimal sperm parametreleri incelendi. CP enjeksiyonu, sperm motilitesi ve yoğunluğunu önemli derecede (P<0.05) düşürdü ve ölü spermatozoa sayısını artırdı. MP + CP uygulaması, CP grubuna kıyasla toplam testis ağırlığı ve toplam cauda epididimis ağırlığını önemli derecede (P<0.05) azalttı, ancak canlı sperm sayısında önemli (P<0.05) bir artışa neden oldu. Sonuç olarak; MP + CP kombinasyonunun, CP grubuna kıyasla toplam testis ve cauda epididimis ağırlığı üzerine olumsuz bir etki göstermekle birlikte, ölü sperm sayısını önemli oranda azalttığı gözlendi

Anahtar Kelime:

Konular: Veterinerlik

Erkek Ratlarda Maclura pomifera Ekstraktının Sisplatin ile Oluşturulmuş Üreme Sistemi Hasarı Üzerine Etkisi

Öz:

In this study, we aimed to investigate the effect of maclura pomifera (MP) (known as cytotoxic, anti-tumoural, anti-inflammatoric, antinociceptive, and anti-oxidative) extract upon the spermatological parameters in Cisplatin (anti-tumoural chemotherapeutic with Reactive Oxygen Species (ROS)- generating side-effects)-treated rats. A total of seventeen male Sprague Dawley rats, aged eight weeks and weighing 250-300 g, were used. Three experimental groups were assigned, as follows; Group 1 (n=5) as control (C): the animals received intraperitoneal (IP) injection of physiological saline and further saline via oral gavage during 5 days before and after the injection, Group 2 (n=6): single IP injection of 7 mg/kg Cisplatin (CP) and saline via oral gavage as above, and Group 3 (n=6): referred as MP + CP, oral administration of 500 mg/kg/day MP extract during 5 days before and after cisplatin (CP) injection, as above. At the end of the experiment, rats were decapitated and their testes and cauda epididymis weights as well as epididymal sperm parameters were analysed. The CP administration caused a significant (P<0.05) decline in sperm motility and density, with a significant increase in dead spermatozoa. The MP + CP combination also led to a significant decline (P<0.05) in the weights of total cauda epididymis and of testes, but significantly improved (P<0.05) sperm survival as compared to the CP alone. In conclusion; the findings suggested that the MP plus CP combination had an adverse effect upon the total weights of testes and cauda epididymis, however the dead sperm rate was markedly lower than the CP alone

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	AKSU E, AKMAN O, özkaraca m, ÖMÜR A, UÇAR Ö (2015). Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats. , 397 - 403.
Chicago	AKSU Emrah Hicazi,AKMAN Orhan,özkaraca mustafa,ÖMÜR Ali Doğan,UÇAR ÖMER Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats. (2015): 397 - 403.
MLA	AKSU Emrah Hicazi,AKMAN Orhan,özkaraca mustafa,ÖMÜR Ali Doğan,UÇAR ÖMER Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats. , 2015, ss.397 - 403.
AMA	AKSU E,AKMAN O,özkaraca m,ÖMÜR A,UÇAR Ö Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats. . 2015; 397 - 403.
Vancouver	AKSU E,AKMAN O,özkaraca m,ÖMÜR A,UÇAR Ö Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats. . 2015; 397 - 403.
IEEE	AKSU E,AKMAN O,özkaraca m,ÖMÜR A,UÇAR Ö "Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats." , ss.397 - 403, 2015.
ISNAD	AKSU, Emrah Hicazi vd. "Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats". (2015), 397-403.

APA	AKSU E, AKMAN O, özkaraca m, ÖMÜR A, UÇAR Ö (2015). Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(3), 397 - 403.
Chicago	AKSU Emrah Hicazi,AKMAN Orhan,özkaraca mustafa,ÖMÜR Ali Doğan,UÇAR ÖMER Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21, no.3 (2015): 397 - 403.
MLA	AKSU Emrah Hicazi,AKMAN Orhan,özkaraca mustafa,ÖMÜR Ali Doğan,UÇAR ÖMER Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.21, no.3, 2015, ss.397 - 403.
AMA	AKSU E,AKMAN O,özkaraca m,ÖMÜR A,UÇAR Ö Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(3): 397 - 403.
Vancouver	AKSU E,AKMAN O,özkaraca m,ÖMÜR A,UÇAR Ö Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(3): 397 - 403.
IEEE	AKSU E,AKMAN O,özkaraca m,ÖMÜR A,UÇAR Ö "Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21, ss.397 - 403, 2015.
ISNAD	AKSU, Emrah Hicazi vd. "Effect of Maclura pomifera Extract on Cisplatin-Induced Damages in Reproductive System of Male Rats". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21/3 (2015), 397-403.