Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder

CABADAK, Hülya; Aykac, Asli; GÖREN, MEHMET ZAFER

Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder

Aslı AYKAÇ, (Yakın Doğu Üniversitesi, KKTC)

Mehmet Zafer GÖREN, (Marmara Üniversitesi, Eczacılık Fakültesi, Eczacılık Anabilim Dalı, İstanbul, Türkiye)

Hülya CABADAK (Marmara Üniversitesi, Tıp Fakültesi, İstanbul, Türkiye)

Türk Biyokimya Dergisi

4 0

Yıl: 2015 Cilt: 40 Sayı: 1 Sayfa Aralığı: 1 - 7 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder

Öz:

B-hücre lenfoma/lösemi-2 (Bcl-2) protein ailesi, proapoptotik (Bax) ve antiapoptotik (Bcl-2) proteinleri arasındaki denge ile kontrol edilen programlı hücre ölümü işleminde mitokondri zar geçirgenliğini düzenlemektedir. Post travmatik stress sıçan modelinde farklı beyin bölgelerinde [Bcl-2]/[Bax] oranını araştırmayı amaçlıyoruz.Metod: Dişi Sprague-Dawley sıçanlar ters 12 s aydınlık/karanlık siklusunda 10 dak. kirli kedi kumuna maruz bırakıldı ve protokol 1 hafta sonra travma çağrıştırıcı (temiz kum) ile tekrarlandı. Sıçanlara intraperitonel tuzlu su, fluoksetin (2.5 mg/kg/day) veya propronolol (10 mg/kg/gün) uygulama dönemleri arasında 7 gün verildi. Travma çağrıştırıcıya maruz bırakmanın ardından yükseltilmiş artı labirent yapıldı. Western blot dorsal hipokampus, frontal korteks ve amigdalar bölge homojenatlarında Bcl-2 ve Bax proteinlerinin miktarını belirlemede kullanıldı.Bulgular: Fluoksetin, ankisiyete indeksleri ve dona kalma sürelerindeki artışları geri çevirdi. Amigdalar bölgede ve frontal korteks de [Bcl-2]/[Bax] oranı travmatik kontrol sıçanlarda anlamlı olarak azaldı (p<0.0001), fakat dorsal hipokampusda azalmadı. Fluoksetin apoptotik değişimleri geri çevirmesine rağmen propranolol çevirmedi ve proapoptotik proteinde artışa sebep oldu. Sonuç: Bu sonuçlarla fluoksetinin nöroprotektif rolü önerilebilir fakat propranolol için önerilemez

Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji

Post-travmatik stres hastalığı modelinde dişi sıçanların farklı beyin bölgelerinde proapoptotik ve antiapoptotik proteinlerin oranındaki değişim

Öz:

The B-cell lymphoma/leukemia-2 (Bcl-2) family of proteins governs mitochondrial membrane permeability where the programmed apoptotic process is controlled by the balance between proapoptotic (Bax) and antiapoptotic (Bcl-2) proteins. We aimed to investigate the [Bcl-2]/[Bax] in different brain regions in a post-traumatic stress disorder rat model.Methods: Female Sprague-Dawley rats were exposed to dirty cat litter (trauma) for 10 min and the protocol was repeated 1 week later with a trauma reminder (clean litter) in reversed 12 h light/dark cycle. The rats received intraperitoneal saline, fluoxetine (2.5 mg/kg/day) or propranolol (10 mg/kg/day) for 7 days between exposure sessions. Following exposure to the trauma reminder, elevated plus maze experiments were done. Immunoblotting was used to quantify [Bcl-2] and [Bax] proteins in the homogenates of the dorsal hippocampus, the frontal cortex and the amygdaloid complex.Results: Fluoxetine reversed the increases in the anxiety indices and the freezing times. In the amygdaloid complex and the frontal cortex, the [Bcl-2]/[Bax] decreased in the traumatized control rats significantly (p<0.0001), but not in the dorsal hippocampus. Although the fluoxetine treatment reversed the apoptotic changes but propranolol failed and caused proapoptotic proteins to increase.Conclusion: These results may suggest a neuroprotective role for fluoxetine but not for propranolol

Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Aykac A, GÖREN M, CABADAK H (2015). Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder. , 1 - 7.
Chicago	Aykac Asli,GÖREN MEHMET ZAFER,CABADAK Hülya Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder. (2015): 1 - 7.
MLA	Aykac Asli,GÖREN MEHMET ZAFER,CABADAK Hülya Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder. , 2015, ss.1 - 7.
AMA	Aykac A,GÖREN M,CABADAK H Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder. . 2015; 1 - 7.
Vancouver	Aykac A,GÖREN M,CABADAK H Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder. . 2015; 1 - 7.
IEEE	Aykac A,GÖREN M,CABADAK H "Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder." , ss.1 - 7, 2015.
ISNAD	Aykac, Asli vd. "Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder". (2015), 1-7.

APA	Aykac A, GÖREN M, CABADAK H (2015). Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder. Türk Biyokimya Dergisi, 40(1), 1 - 7.
Chicago	Aykac Asli,GÖREN MEHMET ZAFER,CABADAK Hülya Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder. Türk Biyokimya Dergisi 40, no.1 (2015): 1 - 7.
MLA	Aykac Asli,GÖREN MEHMET ZAFER,CABADAK Hülya Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder. Türk Biyokimya Dergisi, vol.40, no.1, 2015, ss.1 - 7.
AMA	Aykac A,GÖREN M,CABADAK H Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder. Türk Biyokimya Dergisi. 2015; 40(1): 1 - 7.
Vancouver	Aykac A,GÖREN M,CABADAK H Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder. Türk Biyokimya Dergisi. 2015; 40(1): 1 - 7.
IEEE	Aykac A,GÖREN M,CABADAK H "Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder." Türk Biyokimya Dergisi, 40, ss.1 - 7, 2015.
ISNAD	Aykac, Asli vd. "Altered ratio of proapoptotic and antiapoptotic proteins in different brain regions of female rats in model of post-traumatic stress disorder". Türk Biyokimya Dergisi 40/1 (2015), 1-7.