Yıl: 2016 Cilt: 26 Sayı: 1 Sayfa Aralığı: 127 - 139 Metin Dili: İngilizce

The Effects of Adipose Tissue-Derived Mesenchymal Stem Cell Transplantation During the Acute and Subacute Phases Following Spinal Cord Injury

Öz:
AIM: To investigate the effectiveness of rat adipose tissue-derived (rAT) mesenchymal stem cell (MSC) transplantation on the functional restoration and regeneration of spinal cord injury (SCI).MATERIAl and METhODS: Six of 48 Wistar albino rats were sacrificed to obtain MSCs, and the remaining rats were divided randomly into six groups. SCI was performed using the clip compression method. The control and transplantation groups were injected with physiological saline and a rAT-MSC suspension at the injury sites, respectively. Each animal was evaluated using the Basso, Beattie and Bresnahan (BBB) rating system and sacrificed at 28 days post-injury period (p.i.). The regeneration process was evaluated based on immunostaining against ?3-tubulin, BDNF, CNTF, and CNPase. RESulTS: rAT-MSC transplantation into the SCI site substantially improved the tissue regeneration and functional recovery (p < 0.05). However, the rAT-MSC transplantation at 9 days p.i. was not more efficient on functional recovery than the transplantation immediately after injury. The expression of ?3-tubulin, BDNF and CNTF at the injury site indicated the potential for functional regeneration. COnCluSIOn: The adaptive nature of rat-MSCs enabled the remodulation and regeneration of the lesion site, decreasing the importance of transplantation time in the treatment of SCI
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  • 1. Arboleda D, Forostyak S, Jendelova P, Marekova D, Amemori T, Pivonkova H, Masinova K, Sykova E: Transplantation of predifferentiated adipose-derived stromal cells for the treatment of spinal cord injury. Cell Mol Neurobiol 31: 1113-1122, 2011
  • 2. Basso DM, Beattie MS, Bresnahan JC: A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma 12: 1-21, 1995
  • 3. Bourin P, Bunnell BA, Casteilla L, Dominici M, Katz AJ, March KL, Redl H, Rubin JP, Yoshimura K, Gimble JM: Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: A joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT). Cytotherapy 15(6):641-648, 2013
  • 4. Cakici C, Buyrukcu B, Duruksu G, Haliloglu AH, Aksoy A, Isık A, Uludag O, Ustun H, Subası C, Karaoz E: Recovery of fertility in azoospermia rats after injection of adipose-tissue-derived mesenchymal stem cells: The sperm generation. Biomed Res Int 2013:529589, 2013
  • 5. Choi H, Liao WL, Newton KM, Onario RC, King AM, Desilets FC, Woodard EJ, Eichler ME, Frontera WR, Sabharwal S, Teng YD: Respiratory abnormalities resulting from midcervical spinal cord injury and their reversal by serotonin 1A agonists in conscious rats. J Neurosci 25(18): 4550-4559, 2005
  • 6. Dasari VR, Veeravalli KK, Dinh DH: Mesenchymal stem cells in the treatment of spinal cord injuries: A review. World J Stem Cells 6:120-33, 2014
  • 7. Ek CJ, Habgood MD, Dennis R, Dziegielewska KM, Mallard C, Wheaton B, Saunders NR: Pathological changes in the white matter after spinal contusion injury in the rat. PLoSOne 7(8) e43484, 2012
  • 8. Gosselin RD, Meylan P, Decosterd I: Extracellular microvesicles from astrocytes contain functional glutamate transporters: Regulation by protein kinase C and cell activation. Front Cell Neurosci10; 7:251, 2013
  • 9. Gorgulu A, Kiris T, Unal F, Turkoglu U, Kucuk M, Cobanoglu S: Superoxide dismutase activity and the effects of NBQX and CPP on lipid peroxidation in experimental spinal cord injury.Res Exp Med (Berl) 199(5):285-293, 2000
  • 10. Kabatas S, Teng YD: Potential roles of the neural stem cell in the restoration of the injured spinal cord: Review of the literature. Turk Neurosurg 20(2):103-110, 2010
  • 11. Kamei N, Tanaka N, Oishi Y, Ishikawa M, Hamasaki T, Nishida K, Nakanishi K, Sakai N, Ochi M: Bone marrow stromal cells promoting corticospinal axon growth through the release of humoral factors in organotypic cocultures in neonatal rats. J Neurosurg Spine 6(5):412-419, 2007
  • 12. Kang SK, Shin MJ, Jung JS, Kim YG, Kim CH: Autologous adipose tissue-derived stromal cells for treatment of spinal cord injury. Stem Cells Dev 15:583-594, 2006
  • 13. Kang SK, Yeo JE, Kang KS, Phinney DG: Cytoplasmic extracts from adipose tissue stromal cells alleviates secondary damage by modulating apoptosis and promotes functional recovery following spinal cord injury. Brain Pathol 17:263-275, 2007
  • 14. Karaoz E, Aksoy A, Ayhan S, Sariboyaci AE, Kaymaz F, Kasap M: Characterization of mesenchymal stem cells from rat bone marrow: Ultrastructural properties, differentiation potential and immunophenotypic markers. Histochem Cell Biol 132: 533-546, 2009
  • 15. Karaoz E, Kabatas S, Duruksu G, Okcu A, Subasi C, Ay B, Musluman M, Civelek E: Reduction of lesion in injured rat spinal cord and partial functional recovery of motility after bone marrow derived mesenchymal stem cell transplantation. Turk Neurosurg 22(2):207-217, 2012
  • 16. Lindsay RM: Nerve growth factors (NGF, BDNF) enhance axonal regeneration but are not required for survival of adult sensory neurons. J Neurosci 8:2394-2405, 1988
  • 17. Neirinckx V, Cantinieaux D, Coste C, Rogister B, Franzen R, Wislet-Gendebien S: Concise review: Spinal cord injuries: How could adult mesenchymal and neural crest stem cells take up the challenge? Stem Cells 32:829-843, 2014
  • 18. Oh JS, Park IS, Kim KN, Yoon DH, Kim SH, Ha Y: Transplantation of an adipose stem cell cluster in a spinal cord injury. Neuroreport 23:277-282, 2012
  • 19. Park SS, Lee YJ, Lee SH, Lee D, Choi K, Kim WH, Kweon OK, Han HJ: Functional recovery after spinal cord injury in dogs treated with a combination of Matrigel and neural-induced adiposederived mesenchymal Stem cells. Cytotherapy 14:584-597, 2012
  • 20. Parr AM, Kulbatski I, Wang XH, Keating A, Tator CH: Fate of transplanted adult neural stem/progenitor cells and bone marrowderived mesenchymal stromal cells in the injured adult rat spinal cord and impact on functional recovery. SurgNeurol 70: 600-607, 2008
  • 21. Parr AM, Kulbatski I, Zahir T, Wang X, Yue C, Keating A, Tator CH: Transplanted adult spinal cord-derived neural stem/progenitor cells promote early functional recovery after rat spinal cord injury. Neuroscience 155: 760-770, 2008
  • 22. Peterson WM, Wang Q, Tzekova R, Wiegand SJ: Ciliary neurotrophic factor and stress stimuli activate the Jak-STAT pathway in retinal neurons and glia. J Neurosci 20:4081-4090, 2000
  • 23. Ryu HH, Lim JH, Byeon YE, Park JR, Seo MS, Lee YW, Kim WH, Kang KS, Kweon OK: Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury. J Vet Sci 10:273-284, 2009
  • 24. Sasaki M, Radtke C, Tan AM, Zhao P, Hamada H, Houkin K, Honmou O, Kocsis JD: BDNF-hypersecreting human mesenchymal stem cells promote functional recovery, axonal sprouting, and protection of corticospinal neurons after spinal cord injury. J Neurosci 29:14932-4941, 2009
  • 25. Sonmez E, Kabatas S, Ozen O, Karabay G, Turkoglu S, Ogus E, Yilmaz C, Caner H, Altinors N: Minocycline treatment inhibits lipid peroxidation, preserves spinal cord ultrastructure, and improves functional outcome after traumatic spinal cord injury in the rat. Spine (Phila Pa1976) 38(15):1253-1259,2013
  • 26. Steffenhagen C, Dechant FX, Oberbauer E, Furtner T, Weidner N, Küry P, Aigner L, Rivera FJ: Mesenchymal stem cells prime proliferating adult neural progenitors toward an oligodendrocyte fate. Stem Cells Dev 21:1838-1851, 2012
  • 27. Teng YD, Yu D, Ropper AE, Li J, Kabatas S, Wakeman DR, Wang J, Sullivan MP, Redmond DE Jr, Langer R, Snyder EY, Sidman RL: Functional multipotency of stem cells: A conceptual review of neurotrophic factor-based evidence and its role in translational research. Curr Neuropharmacol 9(4):574-585, 2011
  • 28. Winter CG, Saotome Y, Levison SW, Hirsh D: A role for ciliary neurotrophic factor as an inducer of reactive gliosis, the glial response to central nervous system injury. Proc Natl Acad Sci U S A 92:5865-5869, 1995
  • 29. Zaminy A, Shokrgozar MA, Sadeghi Y, Norouzian M, Heidari MH, Piryaei A: Transplantation of schwann cells differentiated from adipose stem cells improves functional recovery in rat spinal cord injury. Arch Iran Med16:533-541, 2013
APA ARAS Y, SABANCI P, KABATAŞ S, DURUKSU G, SUBASI C, ERGUVAN M, KARAGÖZ E (2016). The Effects of Adipose Tissue-Derived Mesenchymal Stem Cell Transplantation During the Acute and Subacute Phases Following Spinal Cord Injury. Turkish Neurosurgery, 26(1), 127 - 139.
Chicago ARAS Yavuz,SABANCI PULAT AKIN,KABATAŞ SERDAR,DURUKSU GÖKHAN,SUBASI Cansu,ERGUVAN Mine,KARAGÖZ Erdal The Effects of Adipose Tissue-Derived Mesenchymal Stem Cell Transplantation During the Acute and Subacute Phases Following Spinal Cord Injury. Turkish Neurosurgery 26, no.1 (2016): 127 - 139.
MLA ARAS Yavuz,SABANCI PULAT AKIN,KABATAŞ SERDAR,DURUKSU GÖKHAN,SUBASI Cansu,ERGUVAN Mine,KARAGÖZ Erdal The Effects of Adipose Tissue-Derived Mesenchymal Stem Cell Transplantation During the Acute and Subacute Phases Following Spinal Cord Injury. Turkish Neurosurgery, vol.26, no.1, 2016, ss.127 - 139.
AMA ARAS Y,SABANCI P,KABATAŞ S,DURUKSU G,SUBASI C,ERGUVAN M,KARAGÖZ E The Effects of Adipose Tissue-Derived Mesenchymal Stem Cell Transplantation During the Acute and Subacute Phases Following Spinal Cord Injury. Turkish Neurosurgery. 2016; 26(1): 127 - 139.
Vancouver ARAS Y,SABANCI P,KABATAŞ S,DURUKSU G,SUBASI C,ERGUVAN M,KARAGÖZ E The Effects of Adipose Tissue-Derived Mesenchymal Stem Cell Transplantation During the Acute and Subacute Phases Following Spinal Cord Injury. Turkish Neurosurgery. 2016; 26(1): 127 - 139.
IEEE ARAS Y,SABANCI P,KABATAŞ S,DURUKSU G,SUBASI C,ERGUVAN M,KARAGÖZ E "The Effects of Adipose Tissue-Derived Mesenchymal Stem Cell Transplantation During the Acute and Subacute Phases Following Spinal Cord Injury." Turkish Neurosurgery, 26, ss.127 - 139, 2016.
ISNAD ARAS, Yavuz vd. "The Effects of Adipose Tissue-Derived Mesenchymal Stem Cell Transplantation During the Acute and Subacute Phases Following Spinal Cord Injury". Turkish Neurosurgery 26/1 (2016), 127-139.