Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates

Otlu, Baris; TEKEREKOĞLU, Mehmet Sait; KARADEMİR, Fikret; SERİNDAĞ, Ayfer; MANSUR, Ahmet; AY, Selma

Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates

Selma AY, (İnönü Üniversitesi, Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, Malatya, Türkiye)

Ahmet MANSUR, (Yeşilyurt Hasan Çalık Devlet Hastanesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Laboratuvarı, Malatya, Türkiye)

Barış OTLU, (İnönü Üniversitesi, Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, Malatya, Türkiye)

Ayfer SERİNDAĞ, (İnönü Üniversitesi, Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, Malatya, Türkiye)

Mehmet Sait TEKEREKOĞLU, (İnönü Üniversitesi, Tıp Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dalı, Malatya, Türkiye)

Fikret KARADEMİR (Muğla Sıtkı Koçman Üniversitesi, Sağlık Hizmetleri Meslek Yüksek Okulu, Marmaris, Türkiye)

Turgut Özal Tıp Merkezi Dergisi

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Yıl: 2016 Cilt: 23 Sayı: 1 Sayfa Aralığı: 21 - 25 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates

Öz:

Amaç: Karbapenem dirençli Pseudomonas aeruginosa izolatlarında MBL taraması için MBL E test; hızlı ve güvenilir fenotipik test olarak önerilmektedir. Ancak antibiyotik duyarlılık testlerinde MBL E testinde kullanılan farklı marka Müeller- Hinton Agar (MHA) besiyerlerinde karbapenem MİK değerleri ve MBL E test sonuçlarının etkilenebileceğini gösteren çalışmalar mevcuttur. Bu nedenle çalışmamızda 5 farklı marka MHA besiyeri kullanarak, rutin mikrobiyoloji laboratuarlarında MBL E test araştırılmasında kullanılabilecek en uygun marka MHA besiyerini belirlemeyi amaçladık. Gereçler ve Yöntemler: Çalışmada yatan hastalardan izole edilen, karbapenemlere dirençli 29 Pseudomonas aeruginosa izolatları konvansiyonel yöntemler ile tanımlanmıştır. Karbapenem direncini doğrulamak için imipenem ve meropenem E test (AB BIODISK, Solna, İsveç) kullanılmıştır. MBL üretimini belirlemek için MBL E test (AB BIODISK, Solna, İsveç) beş farklı marka (Oxoid, BBL, Merck, Himedia, Plasmatec) Müller Hinton agar (MHA) besiyerinde test edilmiş ve elde edilen sonuçlar Polimeraz Zincir Reaksiyonu sonuçları ile karşılaştırılmıştır. Bulgular:Karbapenemlere dirençli 29 izolatta Polimeraz Zincir Reaksiyonu ile IMP, VIM, GIM, SIM ve SPM tipi metallo beta-laktamaz geni saptanmamıştır. MBL E test ile BBL marka MHA besiyerinde bir izolat (1/29), Oxoid marka MHA besiyerinde iki izolat (2/29), Himedia marka MHA besiyerinde 19 izolat (19/29) Merck marka MHA besiyerinde 21 izolat (21/29), Plasmatec marka MHA besiyerinde 27 izolat (27/29) pozitif sonuç vermiştir. Sonuç: Moleküler tanı yapma olanağı olmayan laboratuvarlarda MBL E test araştırmalarında BBL ve Oxoid marka besiyerlerinin, diğer besiyerlerine oranla daha güvenilir bir şekilde kullanılabileceği sonucuna varılmıştır.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

MBL E Test Araştırmasında 5 Farklı Marka Müller-Hinton Agar Besiyerlerinden Elde Edilen Sonuçların Değerlendirilmesi

Öz:

Aim:MBL E test for MBL screening in carbapenem resistant Pseudomonas aeruginosa isolates is recommended as a fast and reliable phenotypic screening test. However, as it has been put forward by some reserachers, because of variations in antibiotic susceptibility tests or MBL E test with different brands of Müeller- Hinton Agar (MHA) media, the possibility that carbapenem MIK values and MBL E test results can, therefore, be influenced by these media. To this end, we aim to determine the most suitable MHA media to be used in search for MBL E test in routine microbiology laboratories by employing five different brands of MHA media. Materials and Methods:29 carbapenem resistant Pseudomonas aeruginosa strains isolated from hospitalised patients have been used in this study. P.aeruginosa isolates were identified by conventional methods. Imipenem and meropenem E test were used for verification of carbapenem resistance. MBL E test was used for detecting MBL production by five different brands of Mueller Hinton agar plates and the results of these tests were compared with polymerase chain reaction (PCR) results. Results: IMP, VIM, GIM, SIM and SPM type genes were found to be negative with polymerase chain reaction for 29 isolates that were resistant to carbapenem. One isolate with BBL brand MHA, two isolates with Oxoid brand MHA, 19 isolates with Himedia brand MHA, 21 isolates with Merck brand MHA, and 27 isolates with Plasmatec brand MHA gave positive results. Conclusion: The present results indicate that use of BBL or Oxoid brand MHA media, especially in laboratories without any fascilities for molecular diagnosis, can be more reliable compared to other brands.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	AY S, MANSUR A, Otlu B, SERİNDAĞ A, TEKEREKOĞLU M, KARADEMİR F (2016). Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates. , 21 - 25.
Chicago	AY Selma,MANSUR Ahmet,Otlu Baris,SERİNDAĞ Ayfer,TEKEREKOĞLU Mehmet Sait,KARADEMİR Fikret Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates. (2016): 21 - 25.
MLA	AY Selma,MANSUR Ahmet,Otlu Baris,SERİNDAĞ Ayfer,TEKEREKOĞLU Mehmet Sait,KARADEMİR Fikret Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates. , 2016, ss.21 - 25.
AMA	AY S,MANSUR A,Otlu B,SERİNDAĞ A,TEKEREKOĞLU M,KARADEMİR F Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates. . 2016; 21 - 25.
Vancouver	AY S,MANSUR A,Otlu B,SERİNDAĞ A,TEKEREKOĞLU M,KARADEMİR F Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates. . 2016; 21 - 25.
IEEE	AY S,MANSUR A,Otlu B,SERİNDAĞ A,TEKEREKOĞLU M,KARADEMİR F "Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates." , ss.21 - 25, 2016.
ISNAD	AY, Selma vd. "Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates". (2016), 21-25.

APA	AY S, MANSUR A, Otlu B, SERİNDAĞ A, TEKEREKOĞLU M, KARADEMİR F (2016). Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates. Turgut Özal Tıp Merkezi Dergisi, 23(1), 21 - 25.
Chicago	AY Selma,MANSUR Ahmet,Otlu Baris,SERİNDAĞ Ayfer,TEKEREKOĞLU Mehmet Sait,KARADEMİR Fikret Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates. Turgut Özal Tıp Merkezi Dergisi 23, no.1 (2016): 21 - 25.
MLA	AY Selma,MANSUR Ahmet,Otlu Baris,SERİNDAĞ Ayfer,TEKEREKOĞLU Mehmet Sait,KARADEMİR Fikret Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates. Turgut Özal Tıp Merkezi Dergisi, vol.23, no.1, 2016, ss.21 - 25.
AMA	AY S,MANSUR A,Otlu B,SERİNDAĞ A,TEKEREKOĞLU M,KARADEMİR F Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates. Turgut Özal Tıp Merkezi Dergisi. 2016; 23(1): 21 - 25.
Vancouver	AY S,MANSUR A,Otlu B,SERİNDAĞ A,TEKEREKOĞLU M,KARADEMİR F Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates. Turgut Özal Tıp Merkezi Dergisi. 2016; 23(1): 21 - 25.
IEEE	AY S,MANSUR A,Otlu B,SERİNDAĞ A,TEKEREKOĞLU M,KARADEMİR F "Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates." Turgut Özal Tıp Merkezi Dergisi, 23, ss.21 - 25, 2016.
ISNAD	AY, Selma vd. "Evaluation of Metallo Beta Lactamase E test Results from Different Brands of Mueller Hinton Agar Plates". Turgut Özal Tıp Merkezi Dergisi 23/1 (2016), 21-25.