First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey
Yıl: 2016 Cilt: 22 Sayı: 4 Sayfa Aralığı: 529 - 532 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey
Öz: Bu çalışmada balıkçılar tarafından Büyük Menderes Nehri'nden yakalanan 30 adet Avrupa yılan balığında (Anguilla anguilla L.) anisakid nematodların varlığı araştırılmıştır. Yılan balıklarında Raphidascaris acus türü nematoda rastlanmıştır. Raphidascaris acus Türkiye sularında yakalanan yılan balıklarında ilk kez bildirilmiştir. İdentifiye edilen parazitlerin internal transcribed spacer gen bölgeleri (ITS-1 ve ITS-2) ve 5.8S rDNA'sı amplifiye edilerek sekans analizleri gerçekleştirilmiştir. Nükleotid sekans karşılaştırmaları sonucu bu nematodların R. acus olduğu doğrulanmıştır. Yılan balığında tespit edilen R. acus izolatının (GenBank erişim numarası: KT633862) ITS gen bölgeleri (ITS-1 ve ITS-2) ve 5.8S bölgesinin ikili hizalamaları sonucunda Hazar Denizi (KM047505) ve Polonya, Vistula Lagünü'nden (AY603537) izole edilmiş R. acus izolatları ile arasında sırasıyla %0.0 ve 1.9 oranlarında tür içi nükleotit farklılığı saptanmıştır. Bu çalışmada Ege Bölgesi akarsularında sularında yakalanan yılan balıklarında (A. anguilla) saptanan R. acus'un ITS gen bölgeleri (ITS-1 ve ITS-2) ve 5.8S rDNA'sı sekanslanarak ilk kez moleküler karakterizasyonu yapılmıştır.
Anahtar Kelime: Konular:
Ege Bölgesi Akarsularında Yakalanan Avrupa Yılan Balıklarında (Anguilla anguilla Linnaeus, 1758) Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae)'un İlk Moleküler Karakterizasyonu
Öz: In this study, the presence of anisakid nematodes in the European eels (Anguilla anguilla L.) was investigated. A total of 30 specimens of eels were caught by local fishermen from the Buyuk Menderes River, Turkey. Nematoda species of Raphidascaris acus was found from eels. This is the first record of R. acus from A. anguilla from Turkish waters. The ribosomal DNA (rDNA) internal transcribed spacer regions (ITS-1and ITS-2) and 5.8S of this parasitic species was amplified and sequenced. Also, all the nematodes were identified as R. acus based on nucleotide sequence comparisons. Pairwise comparison between the entire ITS regions and 5.8S of the R. acus isolates of A. anguilla (GenBank accession number: KT633862) and other R. acus isolates from Caspian Sea (KM047505), and Vistula Lagoon, Poland (AY603537) showed differences ranging from 0.0 to 1.9% intraspecific nucleotide differences, respectively. With the present study, R. acus infecting A. anguilla caught off the Aegean Region streams were characterized for the first time by sequencing of the rDNA ITS regions and 5.8S.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Moravec F: Parasitic Nematodes of Freshwater Fishes of Europe. Academia and Kluwer, Dordrecht, 1994.
- Moravec F: Metazoan Parasites of Salmonid Fishes of Europe. Academia, Praha, 2004.
- Kir I, Tekin Ozan S: Seasonal distributions and effects of parasites in Pike (Esox Lucius L., 1758) inhabiting the Isikli Dam Lake (Denizli). Turkiye Parazitol Derg, 29 (4): 291-294, 2005.
- Akmirza A, Yardimci RE: Fish parasites of the Sakarya River, Turkey. JADFA, 1, 23-29, 2014.
- Zhu XQ, Gasser RB, Podolska M, Chilton NB: Characterization of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences. Int J Parasitol, 28, 1911-1921, 1998. DOI: 10.1016/S0020- (98)00150-7
- Kellermanns E, Klimpel S, Palm HW: Molecular identification of ascaridoid nematodes from the deep-sea onion-eye grenadier (Macrourus berglax) from the East Greenland Sea. Deep Sea Res Part I, 54, 2194-2202, DOI: 10.1016/j.dsr.2007.09.001
- Klimpel S, Kleinertz S, Hanel R, Rückert S: Genetic variability in Hysterothylacium aduncum, a raphidascarid nematode isolated from sprat (Sprattus sprattus) of different geographical areas of the northeastern Atlantic. Parasitol Res, 101, 1425-1430, 2007. DOI: 10.1007/s00436-007- 0
- Amor N, Farjallah S, Merella P, Said K, Ben Slimane B: Molecular characterization of Hysterothylacium aduncum (Nematoda: Raphidascaridae) from different fish caught off the Tunisian coast based on nuclear ribosomal DNA sequences. Parasitol Res, 109, 1429-1437, 2011. DOI: 1007/s00436-011-2391-7
- Li L, Liu YY, Zhang LP: Morphological and genetic characterization of Hysterothylacium zhoushanensis sp. nov. (Ascaridida: Anisakidae) from the flatfish Pseudorhombus oligodon (Bleeker) (Pleuronectiformes: Paralichthyidae) in the East China Sea. Parasitol Res, 111, 2393-2401, 2012. DOI: 10.1007/s00436-012-3095-3
- Jahantab M, Haseli M, Salehi Z: Morphological and genetic characteristics of the anisakid nematode Raphidascaris acus from the southwest Caspian Sea: Evidence for the existence of sibling species within a species complex. Parasitol Res, 113, 3419-3425, 2014. DOI: 1007/s00436-014-4007-5
- Pekmezci GZ, Bolukbas CS, Gurler AT, Onuk EE: Occurrence and molecular characterization of Hysterothylacium aduncum (Nematoda: Anisakidae) from Merlangius merlangus euxinus and Trachurus trachurus off the Turkish coast of Black Sea. Parasitol Res, 112, 1031-1037, 2013. DOI: 1007/s00436-012-3227-9
- Pekmezci GZ, Yardimci B, Onuk EE Umur S: Molecular characterization of Hysterothylacium fabri (Nematoda: Anisakidae) from Zeus faber (Pisces: Zeidae) caught off the Mediterranean coasts of Turkey based on nuclear ribosomal and mitochondrial DNA sequences. Parasitol Int, 63, 127-131, 2014. DOI: 10.1016/j.parint.2013.10.006
- Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res, 25, 3389-3402, 1997. DOI: 1093/nar/25.17.3389
- Thompson JD, Higgins DG, Gibson TJ: CLUSTALW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res, 22, 4673-4680, 1994. DOI: 10.1093/nar/22.22.4673
- Tamura K, Stecher G, Peterson D, Filipski A, Kumar S: MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol, 30, 2729, 2013. DOI: 10.1093/molbev/mst197
- Felsenstein J: Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783-791, 1985. DOI: 10.2307/2408678
- Moravec F, Kohn A, Fernandes BMM: First record of Raphidascaris (Sprentascaris) hypostomi (Petter et Cassone, 1984) comb. n. and R. (S.) mahnerti (Petter et Cassone, 1984) comb. n. (Nematoda: Anisakidae) from Brazil, with remarks on the taxonomic status of the genus Sprentascaris Petter et Cassone, 1984. Folia Parasitol, 37, 131-140, 1990.
- Moravec F, Nagasawa K: Redescription of Raphidascaris gigi Fujita, (Nematoda: Anisakidae), a parasite of freshwater fishes in Japan. Syst Parasitol, 52, 193-198, 2002. DOI: 10.1023/A:1015785602488
- Pérez-i-García D, Constenla M, Carrassón M, Montero FE, Soler- Membrives A, González-Solís D: Raphidascaris (Raphidascaris) macrouri n. sp. (Nematoda: Anisakidae) from two deep-sea macrourid fishes in the Western Mediterranean: Morphological and molecular characterisations. Parasitol Int, 64, 345-352, 2015. DOI: 10.1016/j.parint.2015.05.002
- Kijewska A, Rokicki J, Sitko J, Wegrzyn G: Ascaridoidea: a simple DNA assay for identification of 11 species infecting marine and freshwater fish, mammals, and fish-eating birds. Exp Parasitol, 101, 35-39, 2002. DOI: 1016/S0014-4894(02)00031-0
- Zhu XQ, Gasser RB, Jacobs DE, Hung GC, Chilton NB: Relationships among some ascaridoid nematodes based on ribosomal DNA sequence data. Parasitol Res, 86, 738-744, 2000. DOI: 10.1007/PL00008561
- Damin L, Heqing H: Heliconema minnanensis n sp. (Physalopteroidae: Physalopteridae) and Raphidascaris trichiuri (Yin and Zhang) n. comb. (Ascaridoidea: Anisakidae) in marine fishes. J Parasitol, 87, 1090-1094, DOI: 10.1645/0022-3395(2001)087[1090:HMNSPP]2.0.CO;2
- Li L, Liu YY, Liu BC, Zhang LP: Morphological and molecular evidence for a new species of the genus Raphidascaris (Nematoda: Anisakidae) from marine fishes from the South China Sea. Parasitol Res, 110, 1473- , 2012. DOI: 10.1007/s00436-011-2650-7
- Xu Z, Zhang LP, Liu BC, Li L: Morphological and molecular characterization of Raphidascaris (Ichthyascaris) lophii (Wu, 1949) (Nematoda, Anisakidae) from marine fishes from China, with a key to the species of the subgenus Ichthyascaris. Acta Parasitol, 57, 316-322, 2012. DOI: 10.2478/s11686-012-0037-2
| APA | SIMSEK E, CILOGLU A, AYPAK S, YILDIRIM A, ALDEMİR O, UNLU A, PEKMEZCİ G (2016). First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey. , 529 - 532. |
| Chicago | SIMSEK EMRAH,CILOGLU ARIF,AYPAK Süleyman,YILDIRIM Alparslan,ALDEMİR Osman Selçuk,UNLU Ahmet Hakan,PEKMEZCİ GÖKMEN ZAFER First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey. (2016): 529 - 532. |
| MLA | SIMSEK EMRAH,CILOGLU ARIF,AYPAK Süleyman,YILDIRIM Alparslan,ALDEMİR Osman Selçuk,UNLU Ahmet Hakan,PEKMEZCİ GÖKMEN ZAFER First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey. , 2016, ss.529 - 532. |
| AMA | SIMSEK E,CILOGLU A,AYPAK S,YILDIRIM A,ALDEMİR O,UNLU A,PEKMEZCİ G First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey. . 2016; 529 - 532. |
| Vancouver | SIMSEK E,CILOGLU A,AYPAK S,YILDIRIM A,ALDEMİR O,UNLU A,PEKMEZCİ G First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey. . 2016; 529 - 532. |
| IEEE | SIMSEK E,CILOGLU A,AYPAK S,YILDIRIM A,ALDEMİR O,UNLU A,PEKMEZCİ G "First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey." , ss.529 - 532, 2016. |
| ISNAD | SIMSEK, EMRAH vd. "First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey". (2016), 529-532. |
| APA | SIMSEK E, CILOGLU A, AYPAK S, YILDIRIM A, ALDEMİR O, UNLU A, PEKMEZCİ G (2016). First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22(4), 529 - 532. |
| Chicago | SIMSEK EMRAH,CILOGLU ARIF,AYPAK Süleyman,YILDIRIM Alparslan,ALDEMİR Osman Selçuk,UNLU Ahmet Hakan,PEKMEZCİ GÖKMEN ZAFER First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 22, no.4 (2016): 529 - 532. |
| MLA | SIMSEK EMRAH,CILOGLU ARIF,AYPAK Süleyman,YILDIRIM Alparslan,ALDEMİR Osman Selçuk,UNLU Ahmet Hakan,PEKMEZCİ GÖKMEN ZAFER First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.22, no.4, 2016, ss.529 - 532. |
| AMA | SIMSEK E,CILOGLU A,AYPAK S,YILDIRIM A,ALDEMİR O,UNLU A,PEKMEZCİ G First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2016; 22(4): 529 - 532. |
| Vancouver | SIMSEK E,CILOGLU A,AYPAK S,YILDIRIM A,ALDEMİR O,UNLU A,PEKMEZCİ G First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2016; 22(4): 529 - 532. |
| IEEE | SIMSEK E,CILOGLU A,AYPAK S,YILDIRIM A,ALDEMİR O,UNLU A,PEKMEZCİ G "First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22, ss.529 - 532, 2016. |
| ISNAD | SIMSEK, EMRAH vd. "First Molecular Characterization of Raphidascaris acus Bloch, 1779 (Nematoda: Anisakidae) from European eels (Anguilla anguilla Linnaeus, 1758) Caught off the Aegean Region Streams, Turkey". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 22/4 (2016), 529-532. |
