Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene

COMBA, Arzu; Oto, Gökhan; COMBA, BAHAT; mis, leyla; ÖZDEMİR, HÜLYA

Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene

Bahat COMBA, (Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Fizyoloji Bölümü, Van)

Gökhan OTO, (Yüzüncü Yıl Üniversitesi Tıp Fakültesi Farmakoloji Anabilim Dalı,Van)

Leyla MİS, (Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Fizyoloji Bölümü, Van)

Hülya ÖZDEMİR, (Yüzüncü Yıl Üniversitesi Tıp Fakültesi Farmakoloji Anabilim Dalı,Van)

Arzu COMBA (Yüzüncü Yıl Üniversitesi, Dursun Odabaş Tıp Merkezi Üroloji Anabilim Dalı, Van)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

10 3

Yıl: 2016 Cilt: 22 Sayı: 4 Sayfa Aralığı: 539 - 544 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene

Öz:

Bu çalışmada, 3-metilkolatren (3-MC) uygulanan sıçanlarda boraksın (BX) inflamasyon göstergeleri, hematolojik parametreler ve total oksidan (TOS)-antioksidan durumlar (TAS) üzerine etkileri araştırıldı. Çalışmada toplam 24 Wistar Albino sıçan kullanıldı. Sıçanlar her grupta 6'şar adet olacak şekilde 4 gruba ayrıldı. Birinci Grup kontrol grubu olarak ayrıldı. İkinci gruba 25 mg/kg dozunda haftada iki kez ilk 2 hafta 3-MC i.p. yolla uygulandı. Üçüncü gruba BX 300 mg/L/gün dozunda içme suları ile 150 gün boyunca verildi. Dördüncü gruba 3-MC 25 mg/kg dozunda haftada iki kez ilk 2 hafta i.p. yolla uygulandı ve BX 300 mg/L/gün dozunda içme suları ile 150 gün boyunca verildi. Çalışma sonunda kan analizlerinde, diğer gruplarla karşılaştırıldığında 3-MC grubunda tümör nekrozis faktör alfa (TNF-?) ve interlökin 1 beta (IL-1?); 3-MC ve 3-MC+BX gruplarında ise, TOS, oksidatif stres indeksi (OSI) ve trombosit (PLT) seviyeleri istatistiksel önemde artış gösterdi. Kontrol grubuna göre her 3 gruptaki % lenfosit (%LY) seviyeleri yüksek; fakat % nötrofil (%NEU) seviyeleri önemli düzeyde düşük olduğu belirlendi. 3-MC+BX grubunda hemoglobin (HGB) ve hematokrit (HCT) değerleri diğer gruplara gore önemli bir azalma gösterdi (P<=0.05). 3-MC ve 3-MC+BX gruplarındaki ortalama alyuvar hacmi (MCV) diğer gruplarla karşılaştırıldığında önemli bir azalma gösterdi (P<=0.05). Sonuç olarak, 3-MC'ye maruziyet durumunda BX'ın uzun sureli oral kullanımı oksidatif stresi azaltamayabilir, WBC, %LY, %NEU, PLT, HGB, HCT, MCV gibi hematolojik parametreleri değiştirebilir. Fakat bu değişimler fizyolojik sınırlar içersinde kalır. Yinede BX'ın kullanımında demir kullanımına dikkat edilmelidir. Ayrıca, bu dozda BX'ın kullanımı inflamasyon ve kanser göstergeleri olan TNF-?, IL-1?, IL-6 seviyelerini azaltabilir.

Anahtar Kelime:

Konular: Veterinerlik

3-Metilkolatren Uygulanan Sıçanlarda Boraksın İnflamasyon, Hematolojik Parametreler ve Total Oksidan-Antioksidan Durumlar Üzerine Etkileri

Öz:

In this study was investigated effects of borax (BX) on inflammation markers, haematolojical parameters and total oxidant (TOS)-antioxidant status (TAS) in rats applied 3-methylcholanthrene (3-MC). In this research a total of 24 Wistar Albino rats were used. They were divided into 4 groups each containing 6 rats. 1st group was separated as a control group. 3-MC was applied twice a week first 2 weeks 25 mg/kg dose to the 2nd group with i.p. way. BX was given to 3rd group 300mg/L/day dose with drinking water during 150 days. 3-MC was applied twice a week first 2 weeks 25 mg/kg dose with i.p. way and BX were given with drinking water during 150 days to 4th group. At the end of the study blood analysis, tumor necrosis factor alpha (TNF-?) and interleukin 1 beta (IL-1?) levels in 3-MC group; TOS and oxidative stress index (OSI), platelet (PLT) levels in 3-MC and 3-MC+BX groups showed significantly increases when compared to other groups. It was determined that lymphocytes % (LY%) of ever 3 groups were significantly higher; however, neutrophil % (NEU%) were significantly fewer according to control group. Haemoglobin (HGB) and hematocrit (HCT) values of 3-MC+BX groups showed significantly decrease according to other groups (P<=0.05). Mean corpusculer volume (MCV) in 3-MC and 3-MC+BX groups showed significantly decrease when compared to other groups (P<=0.05). As a result, in case of exposure to 3-MC, long-term use of BX with oral ways may not decrease oxidative stress, may changes haematolojical parameters such as, WBC, LY%, NEU%, PLT, HGB, HCT, MCV. However, these changes remain within physiological limits. Even so, in the use of BX should be considered use of iron. Furthermore, BX with the abovementioned dosage may be used to reduce the levels of TNF-?, IL-1?, IL-6 being inflammation and cancer markers.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	COMBA B, Oto G, mis l, ÖZDEMİR H, COMBA A (2016). Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene. , 539 - 544.
Chicago	COMBA BAHAT,Oto Gökhan,mis leyla,ÖZDEMİR HÜLYA,COMBA Arzu Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene. (2016): 539 - 544.
MLA	COMBA BAHAT,Oto Gökhan,mis leyla,ÖZDEMİR HÜLYA,COMBA Arzu Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene. , 2016, ss.539 - 544.
AMA	COMBA B,Oto G,mis l,ÖZDEMİR H,COMBA A Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene. . 2016; 539 - 544.
Vancouver	COMBA B,Oto G,mis l,ÖZDEMİR H,COMBA A Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene. . 2016; 539 - 544.
IEEE	COMBA B,Oto G,mis l,ÖZDEMİR H,COMBA A "Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene." , ss.539 - 544, 2016.
ISNAD	COMBA, BAHAT vd. "Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene". (2016), 539-544.

APA	COMBA B, Oto G, mis l, ÖZDEMİR H, COMBA A (2016). Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22(4), 539 - 544.
Chicago	COMBA BAHAT,Oto Gökhan,mis leyla,ÖZDEMİR HÜLYA,COMBA Arzu Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 22, no.4 (2016): 539 - 544.
MLA	COMBA BAHAT,Oto Gökhan,mis leyla,ÖZDEMİR HÜLYA,COMBA Arzu Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.22, no.4, 2016, ss.539 - 544.
AMA	COMBA B,Oto G,mis l,ÖZDEMİR H,COMBA A Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2016; 22(4): 539 - 544.
Vancouver	COMBA B,Oto G,mis l,ÖZDEMİR H,COMBA A Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2016; 22(4): 539 - 544.
IEEE	COMBA B,Oto G,mis l,ÖZDEMİR H,COMBA A "Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22, ss.539 - 544, 2016.
ISNAD	COMBA, BAHAT vd. "Effects of Borax on Inflammation, Haematological Parameters and Total Oxidant-Antioxidant Status in Rats Applied 3-Methylcholanthrene". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 22/4 (2016), 539-544.