Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model

Aydos, Tolga Reşat; EFE, OĞUZHAN EKİN; Kisa, Ucler; UZUNALİOGLU, Tuba; ATMACA, Hasan Tarık; KUL, Oğuz; BAŞAR, Mehmet Murad

Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model

Tolga Reşat AYDOS, (Başkent Üniversitesi, Tıp Fakültesi, Farmakoloji Anabilim Dalı, Ankara, Türkiye)

Mehmet Murad BAŞAR, (Memorial Şişli Hastanesi, Üroloji Ve Androloji Anabilim Dalı, İstanbul, Türkiye)

Oğuz KUL, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Kırıkkale, Türkiye)

Hasan Tarık ATMACA, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Kırıkkale, Türkiye)

Tuba UZUNALİOGLU, (Kırıkkale Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Kırıkkale, Türkiye)

Üçler KISA, (Kırıkkale Üniversitesi, Tıp Fakültesi, Biyokimya ve Klinik Biyokimya Anabilim Dalı, Kırıkkale, Türkiye)

Oğuzhan Ekin EFE (Başkent Üniversitesi, Tıp Fakültesi, Farmakoloji Anabilim Dalı, Ankara, Türkiye)

Turkish Journal of Medical Sciences

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Yıl: 2014 Cilt: 44 Sayı: 5 Sayfa Aralığı: 749 - 755 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model

Öz:

Background/aim: To investigate the effect of ozone and/or taurine treatment comparatively on testicular damage due to ischemia/ reperfusion (I/R) injury in an experimental torsion model in rats. Materials and methods: Adult Wistar rats with and without torsion/detorsion were used. In order to monitor the effect of ozone and/ or taurine treatment on testicular damage due to I/R injury, following histopathological investigation apoptotic indexes were scored by TUNEL method. Moreover, tumor necrosis factor receptor 1 (TNFR1), caspase 3, caspase 8, endothelial nitric oxide synthase (eNOS), tumor necrosis factor alpha (TNFα), and cytochrome C immunostainings were performed and the levels of malondialdehyde, glutathione peroxidase, superoxide dismutase, catalase, total sulfhydryl, and nitric oxide were determined in the testicular tissue. Results: Intraperitoneal ozone and/or taurine treatment prevented both histopathological damage and increase in the apoptotic index. Torsion did not exert an effect on the levels of TNFα and cytochrome C. Ozone and/or taurine treatment prevented increases in TNFR1, caspase 3, and caspase 8. The level of oxidative stress markers was unchanged. The increases in NO level and eNOS expression were prevented by ozone and/or taurine treatment in I/R groups. Conclusion: Using ozone therapy and/or taurine before reperfusion may be a solution for germ cell degeneration resulting from testicular torsion and related infertility.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Visser AJ, Heyns CF. Testicular function after torsion of the spermatic cord. BJU Int 2003; 92: 200203.
2. Saba M, Morales CR, De Lamirande E, Gagnon C. Morphological and biochemical changes following acute unilateral testicular torsion in prepubertal rats. J Urol 1997; 157: 11491154.
3. Jhunjhunwala JS, Desal A, Kropp KA. Torsion of the spermatic cord: an experimental study. Invest Urol 1976; 13: 318320.
4. Cuzzocrea S, Riley DP, Caputi AP, Salvemini D, Antioxidant therapy: a new pharmacological approach in shock, inflammation, and ischemia/reperfusion injury. Pharmacol Rev 2001; 53: 135159.
5. Romeo C, Antonuccio P, Esposito M, Marini H, Impellizzeri P, Turiaco N, Altavilla D, Bitto A, Zuccarello B, Squadrito F. Raxofelast, a hydrophilic vitamin E-like antioxidant, reduces testicular ischemia-reperfusion injury. Urol Res 2004; 32: 367 371.
6. Bocci V, Zanardi I, Travagli V. Oxygen/ozone as a medical gas mixture. A critical evaluation of the various methods clarifies positive and negative aspects. Med Gas Res 2011; 1: 6.
7. Hernandez FA, Menéndez S, Wong R. Decrease of blood cholesterol and stimulation of antioxidative response in cardiopathy patients treated with endovenous ozone therapy. Free Radic Biol Med 1995; 19: 115119.
8. Valdes RA, Menéndez Cepero S, Gómez Moraleda M, Ley Pozo J. Ozone therapy in the advanced stages of arteriosclerosis obliterans. Angiologia 1993; 45: 146148 (article in Spanish with abstract in English).
9. Hernandez Rosales FA, Calunga Fernández JL, Turrent Figueras J, Menéndez Cepero S, Montenegro Perdomo A. Ozone therapy effects on biomarkers and lung function in asthma. Arch Med Res 2005; 36: 549554.
10. Sagai M, Bocci V. Mechanisms of action involved in ozone therapy: is healing induced via a mild oxidative stress? Medical Gas Research 2011; 1: 29.
11. Huxtable RJ. Physiological action of taurine. Physiol Rev 1992; 72: 101163.
12. Wright CE, Tallan HH, Lin YY, Gaull GE. Taurine: biological update. Annu Rev Biochem 1986; 55: 427453.
13. Wright CE, Lin TT, Syurman JA, Gaull GE. Taurine scavenges oxidized chlorine in biological systems. In: Oja SS, editor. Taurine: Biological Actions and Clinical Perspectives. New York, NY, USA: Alan R Liss; 1985. pp. 137147.
14. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265275.
15. Armstrong D, Al-Awadi F. Lipid peroxidation and retinopathy in streptozotocin-induced diabetes. Free Radic Biol Med 1991; 11: 433436.
16. Miranda KM, Espey MG, Wink DA. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide 2001; 5: 6271.
17. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellmans reagent. Anal Biochem 1968; 25: 192205.
18. Lysiak JJ, Turner SD, Nguyen QA, Singbartl K, Ley K, Turner TT. Essential role of neutrophils in germ cell-specific apoptosis following ischemia/reperfusion injury of the mouse testis. Biol Reprod 2001; 65: 718725.
19. Fernández Iglesias A, González Núñez L, Calunga Fernández JL, Rodríguez Salgueiro S, Santos Febles E. Ozone postconditioning in renal ischaemia-reperfusion model. Functional and morphological evidences. Nefrologia 2011; 31: 464470.
20. Oztosun M, Akgul EO, Cakir E, Cayci T, Uysal B, Ogur R, Ozcan A, Ozgurtas T, Guven A, Korkmaz A. The effects of medical ozone therapy on renal ischemia/reperfusion injury. Ren Fail 2012; 34: 912925.
21. Elvis AM, Ekta JS. Ozone therapy: a clinical review. J Nat Sci Biol Med 2011; 2: 6670.
22. Turner TT, Tung KSK, Tomomasa H, Wilson LW. Acute testicular ischemia results in germ cell-specific apoptosis in the rat. Biol Reprod 1997; 57: 12671274.
23. Koksal M, Oğuz E, Baba F, Eren MA, Ciftci H, Demir ME, Kurcer Z, Take G, Aral F, Ocak AR et al. Effects of melatonin on testis histology, oxidative stress and spermatogenesis after experimental testis ischemia-reperfusion in rats. Eur Rev Med Pharmacol Sci 2012; 16: 582588.
24. Lysiak JJ, Nguyen QA, Turner TT. Peptide and nonpeptide reactive oxygen scavengers provide partial rescue of the testis after torsion. J Androl 2002; 23: 400409.
25. Mathur PP, DCruz SC. The effect of environmental contaminants on testicular function. Asian J Androl 2011; 13: 585591.
26. Tripathi R, Mishra DP, Shaha C. Male germ cell development: turning on the apoptotic pathways. J Reprod Immunol 2009; 83: 3135.
27. Bonfoco E, Krainc D, Ankarcrona M, Nicotera P, Lipton SA. Apoptosis and necrosis: two distinct events induced, respectively, by mild and intense insults with N-methyl-Daspartate or nitric oxide/superoxide in cortical cell cultures. Proc Natl Acad Sci USA 1995; 92: 71627166.

APA	Aydos T, BAŞAR M, KUL O, ATMACA H, UZUNALİOGLU T, Kisa U, EFE O (2014). Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model. , 749 - 755.
Chicago	Aydos Tolga Reşat,BAŞAR Mehmet Murad,KUL Oğuz,ATMACA Hasan Tarık,UZUNALİOGLU Tuba,Kisa Ucler,EFE OĞUZHAN EKİN Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model. (2014): 749 - 755.
MLA	Aydos Tolga Reşat,BAŞAR Mehmet Murad,KUL Oğuz,ATMACA Hasan Tarık,UZUNALİOGLU Tuba,Kisa Ucler,EFE OĞUZHAN EKİN Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model. , 2014, ss.749 - 755.
AMA	Aydos T,BAŞAR M,KUL O,ATMACA H,UZUNALİOGLU T,Kisa U,EFE O Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model. . 2014; 749 - 755.
Vancouver	Aydos T,BAŞAR M,KUL O,ATMACA H,UZUNALİOGLU T,Kisa U,EFE O Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model. . 2014; 749 - 755.
IEEE	Aydos T,BAŞAR M,KUL O,ATMACA H,UZUNALİOGLU T,Kisa U,EFE O "Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model." , ss.749 - 755, 2014.
ISNAD	Aydos, Tolga Reşat vd. "Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model". (2014), 749-755.

APA	Aydos T, BAŞAR M, KUL O, ATMACA H, UZUNALİOGLU T, Kisa U, EFE O (2014). Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model. Turkish Journal of Medical Sciences, 44(5), 749 - 755.
Chicago	Aydos Tolga Reşat,BAŞAR Mehmet Murad,KUL Oğuz,ATMACA Hasan Tarık,UZUNALİOGLU Tuba,Kisa Ucler,EFE OĞUZHAN EKİN Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model. Turkish Journal of Medical Sciences 44, no.5 (2014): 749 - 755.
MLA	Aydos Tolga Reşat,BAŞAR Mehmet Murad,KUL Oğuz,ATMACA Hasan Tarık,UZUNALİOGLU Tuba,Kisa Ucler,EFE OĞUZHAN EKİN Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model. Turkish Journal of Medical Sciences, vol.44, no.5, 2014, ss.749 - 755.
AMA	Aydos T,BAŞAR M,KUL O,ATMACA H,UZUNALİOGLU T,Kisa U,EFE O Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model. Turkish Journal of Medical Sciences. 2014; 44(5): 749 - 755.
Vancouver	Aydos T,BAŞAR M,KUL O,ATMACA H,UZUNALİOGLU T,Kisa U,EFE O Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model. Turkish Journal of Medical Sciences. 2014; 44(5): 749 - 755.
IEEE	Aydos T,BAŞAR M,KUL O,ATMACA H,UZUNALİOGLU T,Kisa U,EFE O "Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model." Turkish Journal of Medical Sciences, 44, ss.749 - 755, 2014.
ISNAD	Aydos, Tolga Reşat vd. "Effects of ozone therapy and taurine on ischemia/reperfusion-induced testicular injury in a rat testicular torsion model". Turkish Journal of Medical Sciences 44/5 (2014), 749-755.