Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells

NALBANT ALDANMAZ, AYTEN; KANT, Melis; Akgül, Bünyamin

Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells

Melis KANT, (İzmir Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İzmir, Türkiye)

Bünyamin AKGÜL, (İzmir Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İzmir, Türkiye)

AYTEN NALBANT ALDANMAZ (İzmir Yüksek Teknoloji Enstitüsü, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, İzmir, Türkiye)

Turkish Journal of Biology

4 0

Yıl: 2015 Cilt: 39 Sayı: 1 Sayfa Aralığı: 104 - 110 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells

Öz:

Apoptosis can be used to manipulate host cells by bacterial products such as bacterial heat shock proteins (Hsp). One of the virulence factors of periodontal pathogen Aggregatibacter actinomycetemcomitans is heat shock protein GroEL (AaGroEL), which has been shown to interact with host cells. AaGroEL (Hsp64) also has the potential to modulate immune system cells. In this study we used endogenous AaGroEL protein as an antigen to study bacterial Hsp-induced apoptosis in different immune system cells. Human peripheral blood mononuclear cells and cell lines were cultured with different doses (50-1000 ng/mL) of endogenous AaGroEL at various time points. Apoptosis of the cells was measured by Annexin V and 7AAD labeling. Apoptotic cells were analyzed by flow cytometry. Our data suggested that AaGroEL-responding primary CD8+ T cells were more susceptible to apoptosis than CD4+ T cells. Furthermore, the magnitude of apoptosis in the Jurkat T cell line was higher than that in primary CD8+ T cells. There was no statistically significant level of apoptosis in the chronic myeloid leukemia (K562) cell line, which belongs to myeloid lineages. Thus, A. actinomycetemcomitans GroEL protein has more potent apoptotic effect on cells that are derived from a lymphoid progenitor.

Anahtar Kelime:

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KANT M, Akgül B, NALBANT ALDANMAZ A (2015). Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells. , 104 - 110.
Chicago	KANT Melis,Akgül Bünyamin,NALBANT ALDANMAZ AYTEN Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells. (2015): 104 - 110.
MLA	KANT Melis,Akgül Bünyamin,NALBANT ALDANMAZ AYTEN Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells. , 2015, ss.104 - 110.
AMA	KANT M,Akgül B,NALBANT ALDANMAZ A Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells. . 2015; 104 - 110.
Vancouver	KANT M,Akgül B,NALBANT ALDANMAZ A Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells. . 2015; 104 - 110.
IEEE	KANT M,Akgül B,NALBANT ALDANMAZ A "Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells." , ss.104 - 110, 2015.
ISNAD	KANT, Melis vd. "Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells". (2015), 104-110.

APA	KANT M, Akgül B, NALBANT ALDANMAZ A (2015). Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells. Turkish Journal of Biology, 39(1), 104 - 110.
Chicago	KANT Melis,Akgül Bünyamin,NALBANT ALDANMAZ AYTEN Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells. Turkish Journal of Biology 39, no.1 (2015): 104 - 110.
MLA	KANT Melis,Akgül Bünyamin,NALBANT ALDANMAZ AYTEN Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells. Turkish Journal of Biology, vol.39, no.1, 2015, ss.104 - 110.
AMA	KANT M,Akgül B,NALBANT ALDANMAZ A Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells. Turkish Journal of Biology. 2015; 39(1): 104 - 110.
Vancouver	KANT M,Akgül B,NALBANT ALDANMAZ A Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells. Turkish Journal of Biology. 2015; 39(1): 104 - 110.
IEEE	KANT M,Akgül B,NALBANT ALDANMAZ A "Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells." Turkish Journal of Biology, 39, ss.104 - 110, 2015.
ISNAD	KANT, Melis vd. "Endogenous heat shock protein GroEL of A. actinomycetemcomitans preferentially targets primary human CD8+ T cells". Turkish Journal of Biology 39/1 (2015), 104-110.