Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat

Elvan ANADOL, (Gazi Üniversitesi, Laboratuvar Hayvancılık ve Deneysel Araştırmalar Merkezi, Ankara, Türkiye)
Gul Fatma Yarim, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Samsun, Türkiye)
Nilgün GÜLTİKEN, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Doğum ve Jinekoloji Anabilim Dalı , Samsun, Türkiye)
Filiz KAZAK (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Samsun, Türkiye)
Harran Üniversitesi Veteriner Fakültesi Dergisi
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Yıl: 2016 Cilt: 5 Sayı: 2 Sayfa Aralığı: 124 - 128 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat

Öz:
Bu çalışmanın amacı, ovaryohisterektominin (OHE) ratlarda oksidatif stres üzerine etkilerinin araştırılmasıdır. Bu çalışmada ağırlıkları 200-250 gr, toplam 12 dişi Sprague Dawley rat kullanıldı. Ksilazin (5 mg/kg, i.m.) ve ketamin (45 mg/kg, i.m.) anestezisi altındaki 6 rata OHE (grup I) ve diğer 6 rata da sham operasyonu (grup II) yapıldı. Her iki gruptan dapreoperatif dönemde ve cerrahi sonrası 1. ve 10. günlerde kan örnekleri alındı. Serum malondialdehit (MDA) konsantrasyonu ile süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GPx) aktiviteleri spektrofotometrik yöntem ile ölçüldü. Cerrahi sonrası 1. gün, grup I'in MDA konsantrasyonu artarken, SOD ve GPx aktiviteleri grup II'ye göre azaldı. Benzer olarak, grup I'in MDA konsantrasyonu artarken, SOD ve GPx aktiviteleri grup II'ye göre azaldı, ancak cerrahi sonrası 10. gündeki bu fark istatistiksel olarak anlamlı değildi. Sonuç olarak, bulgularımız ovaryohisterektominin oksidatif stresle ilişkili olduğunu gösterdi.
Anahtar Kelime:

Konular: Ziraat Mühendisliği

Ovaryohisterektominin Ratlarda Bazı Oksidatif Stres Parametreleri Üzerine Etkisi

Öz:
The purpose of the present study was to evaluate the effect of ovariohysterectomy (OHE) on oxidative stress in rats. A total of 12 female Sprague Dawley rats weighing 200-250 g were included in the study. Six rats underwent OHE (group I) and the other 6 rats sham operation (group II) under xylazine (5 mg/kg, i.m.) and ketamine (45 mg/kg, i.m.) anesthesia. In both groups, blood samples were collected preoperatively, day 1 and 10th days after OHE. Serum concentration of malondialdehyde (MDA) and activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) were measured by spectrophotometric methods. The concentration of MDA increased while activities of SOD and GPx decreased in group I compared to group II on day 1 after the surgery. Similarly, the concentration of MDA was higher while activities of SOD and GPx appeared to be lower in group I than that of group II, although the differences were not statistically significant on the 10th day after surgery. In conclusion, our findings indicated that ovariohysterectomy is related with oxidative stress in the rat.
Anahtar Kelime:

Konular: Ziraat Mühendisliği
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ANADOL E, Yarim G, GÜLTİKEN N, Kazak F (2016). Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat. , 124 - 128.
Chicago ANADOL ELVAN,Yarim Gul Fatma,GÜLTİKEN Nilgün,Kazak Filiz Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat. (2016): 124 - 128.
MLA ANADOL ELVAN,Yarim Gul Fatma,GÜLTİKEN Nilgün,Kazak Filiz Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat. , 2016, ss.124 - 128.
AMA ANADOL E,Yarim G,GÜLTİKEN N,Kazak F Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat. . 2016; 124 - 128.
Vancouver ANADOL E,Yarim G,GÜLTİKEN N,Kazak F Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat. . 2016; 124 - 128.
IEEE ANADOL E,Yarim G,GÜLTİKEN N,Kazak F "Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat." , ss.124 - 128, 2016.
ISNAD ANADOL, ELVAN vd. "Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat". (2016), 124-128.
APA ANADOL E, Yarim G, GÜLTİKEN N, Kazak F (2016). Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat. Harran Üniversitesi Veteriner Fakültesi Dergisi, 5(2), 124 - 128.
Chicago ANADOL ELVAN,Yarim Gul Fatma,GÜLTİKEN Nilgün,Kazak Filiz Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat. Harran Üniversitesi Veteriner Fakültesi Dergisi 5, no.2 (2016): 124 - 128.
MLA ANADOL ELVAN,Yarim Gul Fatma,GÜLTİKEN Nilgün,Kazak Filiz Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat. Harran Üniversitesi Veteriner Fakültesi Dergisi, vol.5, no.2, 2016, ss.124 - 128.
AMA ANADOL E,Yarim G,GÜLTİKEN N,Kazak F Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat. Harran Üniversitesi Veteriner Fakültesi Dergisi. 2016; 5(2): 124 - 128.
Vancouver ANADOL E,Yarim G,GÜLTİKEN N,Kazak F Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat. Harran Üniversitesi Veteriner Fakültesi Dergisi. 2016; 5(2): 124 - 128.
IEEE ANADOL E,Yarim G,GÜLTİKEN N,Kazak F "Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat." Harran Üniversitesi Veteriner Fakültesi Dergisi, 5, ss.124 - 128, 2016.
ISNAD ANADOL, ELVAN vd. "Effect of Ovariohysterectomy on Some Oxidative Stress Markers in the Rat". Harran Üniversitesi Veteriner Fakültesi Dergisi 5/2 (2016), 124-128.
Sayfa Oluşturulma Tarihi
23-04-2024 0:59

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