Some forest and agricultural wastes of Eastern Black Sea Region of Turkey were subjected to mushroom (Pleurotus flori-da) cultivation in this study. 1:1, 1:3 and 3:1 (w:w) mixtures were prepared mainly by tree leaves wood wastes of timber workshops, cupola of nut trees (NC) and leaves (NL), corn stalks (CS), waste tea leaves (WTL) of tea factories, wheat straw (WS) andwaste paper(WP). P.floridaof which the strain was numbered darmycel by Fungi Perfect co. (USA) was used in inoculations of pre-pared series of substrates after being sterillized in an autoclave by direct vapor. Results indicated that wood waste yield highest mushroom production as wood waste, waste tea leaves (WW+WTL) based on drysubstrate weight, in a mixture of 3:1 (w:w) with waste tea leaves which reportedly exceeds 10.000 ton and its value as lignocel-lulosic source, it s importance can be estimated in utilization as substrate of mushroom cultivation for forest villagers. Other region-al agricultural and forest wastes were also gave remarkable yieldvalues. In P.floridacultivation corn stalk and cupola and nutincreased the yield values when used with wood wastes as mixtures presence of wood waste in the prepared mixtures with wheatstraw and corn stalks improved the quality of properties of fruit body as cupola of nut resulted in smaller caps in diameter. Furtherstudies are being continued in strain development of different Pleurotusspecies and increasing mushroom yield by many activatorsand additives for the region.