Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats

YENİEL, Özgür; ZEKİOĞLU, Osman; TURAN, Volkan; AKMAN, LEVENT; ERGENOĞLU, Ahmet Mete; DEMİRTAŞ, Gülşah; Ercan, Gülinnaz; TEREK, Mustafa Çoşkun

Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats

Ahmet Mete ERGENOĞLU, (Ege Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, İzmir, Türkiye)

Özgür YENİEL, (Ege Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, İzmir, Türkiye)

Volkan TURAN, (Ege Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, İzmir, Türkiye)

Gülşah DEMİRTAŞ, (Ege Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, İzmir, Türkiye)

Levent AKMAN, (Ege Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, İzmir, Türkiye)

Mustafa Çoşkun TEREK, (Ege Üniversitesi, Tıp Fakültesi, Kadın Hastalıkları ve Doğum Anabilim Dalı, İzmir, Türkiye)

Gülinnaz ERCAN, (Ege Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, İzmir, Türkiye)

Osman ZEKİOĞLU (Ege Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, İzmir, Türkiye)

GORM:Gynecology Obstetrics & Reproductive Medicine

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Yıl: 2014 Cilt: 20 Sayı: 1 Sayfa Aralığı: 29 - 33 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats

Öz:

AMAÇ: Ratlarda siklofosfamid ve sisplatinin over rezervi üzerine etkisini; primordial follikül sayısı, oksidan/anti-oksidan durum, TNF-alfa ve 8-OH deguanozin ölçümleri ile değerlerlendirmektir. GEREÇ VE YÖNTEM: Otuz Wistar rat üç gruba ayrılmıştır. Çalışma Ege Üniversitesi Tıp Fakültesi'nde yapılmıştır. Sisplatin 5 mg/kg dozda (sisplatin grup, n=10), siklofosfamid 6 mg/kg dozda (siklofosfamid grup, n=10) ve kontrol grubuna sadece steril salin (kontrol grup, n=10) uygulanmıştır. Tüm enjeksiyonlar tek doz ve intraperitoneal olarak uygulanmıştır. Yedinci günün sonunda intrakardiyak yoldan kan örnekleri alınmış ve her iki over çıkarılmıştır. Primordial follikül sayısı, TNFalfa, 8-OH deoksiguanozin, malondialdehit (MDA) ve katalaz enzim aktivite düzeyleri değerlendirilmiştir. BULGULAR: Primordial follikül sayısı kontrol grubunda, sisplatin grubunda ve siklofosfamid grubunda sırasıyla 27,5±4,7; 17,3±2,1; 17±1,4 idi (p=0,156). TNF-alfa düzeyi sisplatin grubunda anlamlı olarak yüksek olmasına rağmen (p<0,001), 8- OH deoksiguanozin düzeyinde üç grup arasında anlamlı farklılık yoktu (p=0,431). Diğer gruplar ile karşılaştırıldığında, sisplatin grubunda MDA düzeyi anlamlı olarak düşük (p<0,001) iken katalaz enzim aktivitesi istatiksel anlamlı farklılık göstermemekteydi. SONUÇ: Belirtilen dozlarda kullanılan kemoterapötik ilaçlarda az düzeyde primordial folliküllerde azalma görülmüştür. Artmış TNF-alfa ve MDA düzeyleri, over dokusu hasarı gelişmesinde rol oynayabilir.

Anahtar Kelime:

Konular: Kadın Hastalıkları ve Doğum

Ratlarda Kemoterapinin Over Rezervi Üzerine Etkilerinin Değerlendirilmesi

Öz:

OBJECTIVE: To determine the effect of cyclophosphamide and cisplatin on ovarian reserve in rats by the way of counting primordial follicle and assessing TNF-alpha, 8-OH deguanosine and oxidant/ antioxidant status. STUDY DESIGN: Thirty Wistar rats were divided into three groups. Cisplatin was administered at a dose of 5 mg/kg (Cisplatin group, n=10), cyclophosphamide was administered at a dose of 6 mg/kg (Cyclophosphamide group, n=10) and control group was only given sterile saline (Control group, n=10) in Ege University Faculty of Medicine. All injections were administered intraperitoneally at a single dose. At the end of the 7th day blood samples were drawn by intracardiac aspiration and bilateral extirpation of ovaries were performed. The number of primordial follicles, levels of TNF-alpha, 8-OH deoxyguanosine, malondialdehyde (MDA) and catalase enzyme activities were evaluated. RESULTS: The number of primordial follicles in the control group, cisplatin group and cyclophosphamide group were 27.5±4.7;17.3±2.1;17±1.4, respectively (p=0.156). While the levels of TNF-alpha were significantly higher in cisplatin group (p<0,001), 8-OH deoxyguanosine levels did not have significant difference among three groups (p=0.431). The levels of MDA were significantly lower compared to the other groups (p<0.001); however, the catalase enzyme activities did not show statistically significant difference. CONCLUSIONS: Insignificant depletion of primordial follicles was observed by using specified doses of chemotherapeutics. An increase in TNF-alpha and MDA levels may play a role in the development of ovarian tissue damage.

Anahtar Kelime:

Konular: Kadın Hastalıkları ve Doğum

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ERGENOĞLU A, YENİEL Ö, TURAN V, DEMİRTAŞ G, AKMAN L, TEREK M, Ercan G, ZEKİOĞLU O (2014). Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats. , 29 - 33.
Chicago	ERGENOĞLU Ahmet Mete,YENİEL Özgür,TURAN Volkan,DEMİRTAŞ Gülşah,AKMAN LEVENT,TEREK Mustafa Çoşkun,Ercan Gülinnaz,ZEKİOĞLU Osman Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats. (2014): 29 - 33.
MLA	ERGENOĞLU Ahmet Mete,YENİEL Özgür,TURAN Volkan,DEMİRTAŞ Gülşah,AKMAN LEVENT,TEREK Mustafa Çoşkun,Ercan Gülinnaz,ZEKİOĞLU Osman Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats. , 2014, ss.29 - 33.
AMA	ERGENOĞLU A,YENİEL Ö,TURAN V,DEMİRTAŞ G,AKMAN L,TEREK M,Ercan G,ZEKİOĞLU O Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats. . 2014; 29 - 33.
Vancouver	ERGENOĞLU A,YENİEL Ö,TURAN V,DEMİRTAŞ G,AKMAN L,TEREK M,Ercan G,ZEKİOĞLU O Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats. . 2014; 29 - 33.
IEEE	ERGENOĞLU A,YENİEL Ö,TURAN V,DEMİRTAŞ G,AKMAN L,TEREK M,Ercan G,ZEKİOĞLU O "Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats." , ss.29 - 33, 2014.
ISNAD	ERGENOĞLU, Ahmet Mete vd. "Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats". (2014), 29-33.

APA	ERGENOĞLU A, YENİEL Ö, TURAN V, DEMİRTAŞ G, AKMAN L, TEREK M, Ercan G, ZEKİOĞLU O (2014). Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats. GORM:Gynecology Obstetrics & Reproductive Medicine, 20(1), 29 - 33.
Chicago	ERGENOĞLU Ahmet Mete,YENİEL Özgür,TURAN Volkan,DEMİRTAŞ Gülşah,AKMAN LEVENT,TEREK Mustafa Çoşkun,Ercan Gülinnaz,ZEKİOĞLU Osman Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats. GORM:Gynecology Obstetrics & Reproductive Medicine 20, no.1 (2014): 29 - 33.
MLA	ERGENOĞLU Ahmet Mete,YENİEL Özgür,TURAN Volkan,DEMİRTAŞ Gülşah,AKMAN LEVENT,TEREK Mustafa Çoşkun,Ercan Gülinnaz,ZEKİOĞLU Osman Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats. GORM:Gynecology Obstetrics & Reproductive Medicine, vol.20, no.1, 2014, ss.29 - 33.
AMA	ERGENOĞLU A,YENİEL Ö,TURAN V,DEMİRTAŞ G,AKMAN L,TEREK M,Ercan G,ZEKİOĞLU O Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats. GORM:Gynecology Obstetrics & Reproductive Medicine. 2014; 20(1): 29 - 33.
Vancouver	ERGENOĞLU A,YENİEL Ö,TURAN V,DEMİRTAŞ G,AKMAN L,TEREK M,Ercan G,ZEKİOĞLU O Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats. GORM:Gynecology Obstetrics & Reproductive Medicine. 2014; 20(1): 29 - 33.
IEEE	ERGENOĞLU A,YENİEL Ö,TURAN V,DEMİRTAŞ G,AKMAN L,TEREK M,Ercan G,ZEKİOĞLU O "Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats." GORM:Gynecology Obstetrics & Reproductive Medicine, 20, ss.29 - 33, 2014.
ISNAD	ERGENOĞLU, Ahmet Mete vd. "Assessment of the Effects of Chemotherapy on Ovarian Reserve in Rats". GORM:Gynecology Obstetrics & Reproductive Medicine 20/1 (2014), 29-33.