Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study

ÖNGER, MEHMET EMİN; GÖÇER, Hasan; Dabak, Nevzat; EMİR, Dilek

Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study

Hasan GÖÇER, (Ondokuz Mayıs Üniversitesi, Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, Samsun, Türkiye)

Dilek EMİR, (Ondokuz Mayıs Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Samsun, Türkiye)

Mehmet Emin ÖNGER, (Ondokuz Mayıs Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Samsun, Türkiye)

Nevzat DABAK (Ondokuz Mayıs Üniversitesi, Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, Samsun, Türkiye)

Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.)

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Yıl: 2017 Cilt: 28 Sayı: 1 Sayfa Aralığı: 13 - 18 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study

Öz:

Amaç: Bu çalışmada sadece teikoplaninin, sadece N asetil sistein (NAC)'in veya her iki bileşenin kombinasyonunun kemik çimentosuyla karıştırılmasının antibiyofilm etkileri gösterildi.Gereç ve yöntemler: Her grupta farklı içeriğe sahip kemik çimentosu ile hazırlanan altışarlı çimento kalıplar kullanılarak toplam dört grup oluşturuldu. Grup 1 (kontrol grup): Herhangi bir ilaç katılmaksızın sadece çimento. Grup 2: 40 g çimento, 400 mg teikoplanin. Grup 3: 40 g çimento, 6 g NAC. Grup 4: 40 g çimento 6 g NAC, 400 mg teikoplanin. Tüm çimento kalıpları Stafilokokkus aureus ile 48 saat 36.5 °C'de enfekte edildi. Bakteri kolonileri seri dilüsyon yöntemiyle sayıldı. Bakteriler taramalı elektron mikroskobik (TEM) görüntüler kullanılarak sayıldı.Bulgular: Bakteri kolonisi sayıları grup 1'de 5.83±1.60 [ortalama koloni oluşturan birim (kob) x 105±standart sapma (SS)], grup 2'de 0.12±0.56, grup 3'te 0.11±0.65 ve grup 4'te 0.01±0.001 idi. Grup 1 ile tüm diğer gruplar arasında (p<0.05) ve grup 4 ile tüm diğer gruplar arasında anlamlı farklılık bulundu (p<0.05). TEM analize göre, bakteri sayıları (ort.±SS) grup 1, 2, 3 ve 4'te sırasıyla 1.88±0.45, 0.75±0.26, 0.21±0.22 ve 0.13±0.25 idi. Grup 1 ile tüm gruplar arasında (p<0.05) ve grup 4 ile tüm gruplar arasında anlamlı farklılık bulundu (p<0.05).Sonuç: N asetil sistein, teikoplanin ve bunların kombinasyonu kontrol grubuna göre biyofilm oluşumunu anlamlı olarak azalttı. Ayrıca, NAC ve teikoplanin kombinasyonu en yüksek antibiyofilm etkisine sahipti

Anahtar Kelime:

Konular: Ortopedi

Teikoplanin, N-asetil sistein veya bunların kombinasyonunu içeren kemik çimentosunun Stafilokokkus aureus biyofilm oluşumuna etkileri: İn vitro bir çalışma

Öz:

Objectives: This study aims to demonstrate the antibiofilm effects of teicoplanin alone, N-acetyl cysteine (NAC) alone, or combination of both compounds when mixed with bone cement.Materials and methods: A total of four groups were formed by using six cement samples in each, prepared with bone cement having different contents in each group. Group 1 (control group): cement alone without any drugs added. Group 2: 40 g cement, 400 mg teicoplanin. Group 3: 40 g cement, 6 g NAC. Group 4: 40 g cement, 6 g NAC, 400 mg teicoplanin. All cement samples were infected with Staphylococcus aureus for 48 hours at 36.5 °C. Bacterial colonies were then counted by serial dilution method. Bacteria were counted using scanning electron microscopic (SEM) images.Results: Counts of bacteria colonies were 5.83±1.60 [mean colony forming unit (cfu) x 105±standard deviation (SD)] in group 1, 0.12±0.56 in group 2, 0.11±0.65 in group 3, and 0.01±0.001 in group 4. Significant difference was found between group 1 and all other groups (p<0.05), and between group 4 and all other groups (p<0.05). According to SEM analysis, counts of bacteria (mean±SD) were 1.88±0.45, 0.75±0.26, 0.21±0.22, and 0.13±0.25 in groups 1, 2, 3, and 4, respectively. Significant difference was found between group 1 and all other groups (p<0.05), and between group 4 and all other groups (p<0.05).Conclusion: N-acetyl cysteine, teicoplanin, and their combination significantly reduced formation of biofilm compared to the control group. Also, combination of NAC and teicoplanin had the highest antibiofilm effect

Anahtar Kelime:

Konular: Ortopedi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	GÖÇER H, EMİR D, ÖNGER M, Dabak N (2017). Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study. , 13 - 18.
Chicago	GÖÇER Hasan,EMİR Dilek,ÖNGER MEHMET EMİN,Dabak Nevzat Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study. (2017): 13 - 18.
MLA	GÖÇER Hasan,EMİR Dilek,ÖNGER MEHMET EMİN,Dabak Nevzat Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study. , 2017, ss.13 - 18.
AMA	GÖÇER H,EMİR D,ÖNGER M,Dabak N Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study. . 2017; 13 - 18.
Vancouver	GÖÇER H,EMİR D,ÖNGER M,Dabak N Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study. . 2017; 13 - 18.
IEEE	GÖÇER H,EMİR D,ÖNGER M,Dabak N "Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study." , ss.13 - 18, 2017.
ISNAD	GÖÇER, Hasan vd. "Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study". (2017), 13-18.

APA	GÖÇER H, EMİR D, ÖNGER M, Dabak N (2017). Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), 28(1), 13 - 18.
Chicago	GÖÇER Hasan,EMİR Dilek,ÖNGER MEHMET EMİN,Dabak Nevzat Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.) 28, no.1 (2017): 13 - 18.
MLA	GÖÇER Hasan,EMİR Dilek,ÖNGER MEHMET EMİN,Dabak Nevzat Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), vol.28, no.1, 2017, ss.13 - 18.
AMA	GÖÇER H,EMİR D,ÖNGER M,Dabak N Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.). 2017; 28(1): 13 - 18.
Vancouver	GÖÇER H,EMİR D,ÖNGER M,Dabak N Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study. Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.). 2017; 28(1): 13 - 18.
IEEE	GÖÇER H,EMİR D,ÖNGER M,Dabak N "Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study." Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.), 28, ss.13 - 18, 2017.
ISNAD	GÖÇER, Hasan vd. "Effects of bone cement loaded with teicoplanin, N-acetylcysteine or their combination on Staphylococcus aureus biofilm formation: an in vitro study". Eklem Hastalıkları ve Cerrahisi Dergisi (Eski Adı: Artroplasti Artroskopik Cerrahi Derg.) 28/1 (2017), 13-18.