Yıl: 2014 Cilt: 8 Sayı: 3 Sayfa Aralığı: 214 - 219 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ

Öz:
Bu çalışmada, bir fungusit olarak kullanılan tebukonazol'ün Cyprinus carpio (L., 1758)'da serum total antioksidan (TAS), oksidan (TOS) ve sialik asit (TSA) düzeyleri üzerine etkisinin araştırılması amaçlandı. Balıkların 15 gün süre ile laboratuar ortamına adaptasyonları sağlandıktan sonra, her grupta 6 adet balık bulunan 3 grup oluşturuldu. Grup I'de bulunan balıklar normal su ortamında, grup II ve III ise sırasıyla 2 ve 3 mg/L tebukonazol eklenen tanklarda 14 gün süre ile bekletildi. Balıklardan kan örnekleri alındı ve serumları ayrıldı. Daha sonra, TAS, TOS ve TSA düzeyleri analiz edildi. Serum TAS düzeylerinin kontrol grubuna kıyasla tebukonazol uygulanan gruplarda azalırken, TOS ve TSA bakımından artışlar olduğu saptandı. Sonuç olarak, tebukonazol uygulamalarının Cyprinus carpio'nun serum TAS, TOS ve TSA düzeyleri üzerinde önemli değişiklikler meydana getirdiği tespit edildi.
Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Investigation of The Serum Total Antioxidant, Oxidant and Sialic Acid Levels of Cyprinus carpio (L. 1758) Treated With Tebuconazole (Fungicide)

Öz:
Investigation of The Serum Total Antioxidant, Oxidant and Sialic Acid Levels of Cyprinus carpio (L. 1758) Treated With Tebuconazole (Fungicide) In the present study was aimed to investigate effects on the levels of serum total antioxidant (TAS), oxidant (TOS) and sialic acid (TSA) in Cyprinus carpio (L., 1758) of tebuconazole used as a fungicide. The fishes were kept in tanks for 15 days in terms of adaptation. After, it was formed three groups each containing 6 fishes. Group II and III were placed into separate tanks containing respectively 2 and 3 mg/L tebuconazole and also group I was placed into normal water for 14 days. It were taken the blood samples from fishes and analyzed the levels of serum TAS, TOS and TSA. Even though the serum TAS levels of Tebuconazole-treated groups were lower compared the control group, in terms of TOS and TSA levels was determined the increases. In conclusion, it was determined that tebuconazole treatments caused important changes on the levels of serum TAS, TOS and TSA in carps.
Anahtar Kelime:

Konular: Genel ve Dahili Tıp
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Al-Sabti, K., (1992). Monitoring the genotoxicity of radiocontaminants in Swedish lakes by fish micronuclei, Cytobios, 70: 101-106.
  • Bayer Crop Science Limited, (2005). Enviromental information sheet folicur® MAPP number 11278. CPA Guidance Notes version 3.©EIS. http://www.bayercropscience.co.uk/ assets/Uploads/Folicur_EIS.pdf (Erişim Tarihi: 01.09.2013).
  • Chaudiere, J., Ferrai-Iliou, R., (1999). Intracellular antioxidants: from chemical to biochemical mechanism, Food and Chemical Toxicology, 37: 949- 962. doi: 10.1016/S0278-6915(99)00090-3
  • Doyotte, A., Cossu, C., Jacquin, M.C., Babutb, M., Vaseural, P., (1997). Antioxidant enzymes, glutathione and lipid peroxidation as relevant biomarkers of experimental or field exposure in the gills and the digestive gland of the freshwater bivalve unio tumidus, Aquatic Toxicology, 39: 93-110. doi: 10.1016/S0166-445X(97)00024-6
  • Eguchi, H., Ikeda, Y., Ookawara, T., Koyota, S., Fujiwara, N., Honke, K., Wang, P.G., Taniguchi, N., Suzuki, K., (2005). Modification of oligosaccharides by reactive oxygen species decreases sialyl lewis x-mediated cell adhesion. Glycobiology, 15: 1094-1101. doi: 10.1093/glycob/cwj003
  • Erdil, A., Tüzün, A., Yönem, A., Erbil, M.K., (2001). Serum sialic acid concentrations in patients with non-insulindependent diabetes mellitus and its relation with retinopathy, Türkiye Klinikleri Journal of Medical Sciences, 21: 349-354.
  • Erel, Ö., (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable abts radical cation, Clinical Biochemistry, 37: 277-285. doi: 10.1016/j.clinbiochem.2003.11.015
  • Erel, Ö., (2005). A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38: 1103-1111. doi: 10.1016/j.clinbiochem.2005.08.008
  • Ferreira, D., Costa da Motta, A., Kreutz, L.C., Tonı, C., Loro, V.L., Barcellos, L.J.G., (2010). Assesment of oxidative stress in Rhamdia quelen exposed to agrichemicals. Chemosphere, 79: 914-921. doi: 10.1016/j.chemosphere.2010.03.024
  • Geldiay, R., Balık, S., (1996). Türkiye tatlısu balıkları. Ege Üniversitesi Su Ürünleri Fakültesi Yayınları, No: 46 Ders Kitabı Dizisi No: 16, s.357-362, Bornova/İzmir.
  • Gönenc, A., Özkan, Y., Torun, M., Şimşek, B., (2001). Plasma malondialdehyde (MDA) levels in breast and lung cancer patients, Journal of Clinical Pharmacy and Therapeutics, 26: 141-144. doi: 10.1046/j.1365-2710.2001.00334.x
  • Halliwell, B., (1995). Antioxidant characterization. Methodology and mechanism, Biochemical Pharmacology, 49: 1341-1348. doi: 10.1016/0006-2952(95)00088-H
  • Henricks, P.A., van Erne-van der Tol, M.E., Verhoef, J., (1982). Partial removal of sialic acid enhances phagocytosis and the generation of superoxide and chemiluminescence by polymorphonuclear leukocytes, The Journal of Immunology, 129: 745-750.
  • Kaya, İ., Karapehlivan, M., Yılmaz, M., Ersan, Y., Koç, E., (2012). Investigation of effects on plasma nitric oxide, malondialdehyde and total sialic acid levels of glyphosate in Kars Creek transcaucasıan barb (Capoeta capoeta [Guldenstaedti, 1773]) in Turkey, Fresenius Environmental Bulletin, 21: 123-126.
  • Kumagai, R., Lu, X., Kassab, G.S., (2009). Role of glycocalyx in flow-induced production of nitric oxide and reactive oxygen species, Free Radical Biology and Medicine, 47: 600-607. doi: 10.1016/j.freeradbiomed.2009.05.034
  • Li, Z., Zlabeka, V., Grabica, R., Lia, P., Machovaa, J., Veliseka, J., Randak, T., (2010). Effects of exposure to sublethal propiconazole on the antioxidant defense system and Na+- K+-ATPase activity in brain of rainbow trout, Oncorhynchus mykiss, Aquatic Toxicology, 98: 297-303. doi: 10.1016/j.aquatox.2010.02.017
  • Livingstone, D.R., (2001). Contaminantstimulated reactive oxygen species production and oxidative damage in aquatic organisms, Marine Pollution Bulletin, 42: 656- 666. doi: 10.1016/S0025-326X(01)00060-1
  • Lloyd, R., (1992). Pollution and freshwater fish. A Buckland Foundation Book, Fishing News Books, Cornwall.
  • Mendes, R., Cardoso, C., Pestana, C., (2009). Measurement of malondialdehyde in fish: A comparison study between HPLC methods and the traditional spectrophotometric test, Food Chemistry, 112: 1038-1045. doi: 10.1016/j.foodchem.2008.06.052
  • Mohammad, A., Akram, R., Shahin, S., Sherkoupeh, N., Ali, R., (2004). Pesticides and oxidative stress: a review, Medical Science Monitor, 10: 141-147.
  • Parvez, S., Raisuddin, S., (2005). Protein carbonyls: novel biomarkers of exposure to oxidative stress-inducing pesticides in freshwater fish Channa punctata (Bloch), Environmental Toxicology and Pharmacology, 20: 112- 117. doi: 10.1016/j.etap.2004.11.002
  • Schauer, R., (1982). Chemistry, metabolism, and biological functions of sialic acids, Advances in Carbohydrate Chemistry and Biochemistry, 40: 131-234. doi: 10.1016/S0065-2318(08)60109-2
  • Selvi, M., Sarıkaya, R., Erkoç, F., (2004). Acute behavioral changes in theguppy (Poecilia reticulata) exposed to temephos, Gazi Üniversitesi Fen Bilimleri Dergisi, 17: 15-19.
  • Stegeman, J.J., Lech, J.J., (1991). Cytochrome P- 450 Monooxygenase systems in aquatic species. carcinogen metabolism and biomarkers for carcinogen and pollutant exposure, Environmental Health Perspectives, 90: 101-109. doi: 10.2307/3430851
  • Sydow, G., (1985). A simplified quick method for determination of sialic acid in serum, Biomedica Biochimica Acta, 44: 1721-1723.
  • Varki, N.M., Varki, A., (2007). Diversity in cell surface sialic acid presentations: implications for biology and disease, Laboratory Investigation, 87: 851-857. doi: 10.1038/labinvest.3700656
APA Kaya İ, YILMAZ M, KOÇ E, DEVECİ H, ERSAN Y, KARAPEHLİVAN M (2014). TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ. , 214 - 219.
Chicago Kaya İnan,YILMAZ MUHİTDİN,KOÇ Evren,DEVECİ HACİ AHMET,ERSAN Yusuf,KARAPEHLİVAN Mahmut TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ. (2014): 214 - 219.
MLA Kaya İnan,YILMAZ MUHİTDİN,KOÇ Evren,DEVECİ HACİ AHMET,ERSAN Yusuf,KARAPEHLİVAN Mahmut TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ. , 2014, ss.214 - 219.
AMA Kaya İ,YILMAZ M,KOÇ E,DEVECİ H,ERSAN Y,KARAPEHLİVAN M TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ. . 2014; 214 - 219.
Vancouver Kaya İ,YILMAZ M,KOÇ E,DEVECİ H,ERSAN Y,KARAPEHLİVAN M TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ. . 2014; 214 - 219.
IEEE Kaya İ,YILMAZ M,KOÇ E,DEVECİ H,ERSAN Y,KARAPEHLİVAN M "TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ." , ss.214 - 219, 2014.
ISNAD Kaya, İnan vd. "TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ". (2014), 214-219.
APA Kaya İ, YILMAZ M, KOÇ E, DEVECİ H, ERSAN Y, KARAPEHLİVAN M (2014). TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ. Journal of FisheriesSciences.com, 8(3), 214 - 219.
Chicago Kaya İnan,YILMAZ MUHİTDİN,KOÇ Evren,DEVECİ HACİ AHMET,ERSAN Yusuf,KARAPEHLİVAN Mahmut TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ. Journal of FisheriesSciences.com 8, no.3 (2014): 214 - 219.
MLA Kaya İnan,YILMAZ MUHİTDİN,KOÇ Evren,DEVECİ HACİ AHMET,ERSAN Yusuf,KARAPEHLİVAN Mahmut TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ. Journal of FisheriesSciences.com, vol.8, no.3, 2014, ss.214 - 219.
AMA Kaya İ,YILMAZ M,KOÇ E,DEVECİ H,ERSAN Y,KARAPEHLİVAN M TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ. Journal of FisheriesSciences.com. 2014; 8(3): 214 - 219.
Vancouver Kaya İ,YILMAZ M,KOÇ E,DEVECİ H,ERSAN Y,KARAPEHLİVAN M TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ. Journal of FisheriesSciences.com. 2014; 8(3): 214 - 219.
IEEE Kaya İ,YILMAZ M,KOÇ E,DEVECİ H,ERSAN Y,KARAPEHLİVAN M "TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ." Journal of FisheriesSciences.com, 8, ss.214 - 219, 2014.
ISNAD Kaya, İnan vd. "TEBUKONAZOL (FUNGUSİT) UYGULANAN Cyprinus carpio (L. 1758)'DA SERUM TOTAL ANTİOKSİDAN, OKSİDAN ve SİALİK ASİT DÜZEYLERİNİN İNCELENMESİ". Journal of FisheriesSciences.com 8/3 (2014), 214-219.