Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms
Yıl: 2011 Cilt: 17 Sayı: (A) Sayfa Aralığı: 153 - 158 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms
Öz: Akrep zehirlenmesi hala birçok tropikal ve subtropikal ülkelerde önemli bir sağlık sorunu olmaya devam etmektedir. Akrep sokmalarının tedavisinde, antivenom hala yaygın olarak uygulanmaktadır. Antivenom üretimi için akrep venomları antijen kaynağı olarak kullanılmaktadır. Bu nedenle, venomun kalite kontrolü ve homojenliği çok önemli bir noktadır. Bu çalışmada, Türkiye'de Osmaniye ve Niğde illerinde yetişkin Mesobuthus gibbosus ve Mesobuthus eupeus akrepleri toplanıldı. Ekstraksiyon sonra, venom kompozisyonu tek boyutlu jel elektroforezi (1-DE) kullanılarak analiz edilmiştir. Bütün venom örneklerinde ortak tek protein bandı (70 kDa) bulundu. Ayrıca, Osmaniye ve Niğde illerindeki dişi akreplerin venomunda üç protein bandı (70, 87 ve 100 kDa) tespit edilmiştir.
Anahtar Kelime: Konular:
Mesobuthus eupeus ve Mesobuthus gibbosus Akrep Venom Proteinlerinin Elektroforetik Karşılaştırılması
Öz: Scorpion envenomation still remains a major health problem in many tropical and subtropical countries. Antivenom is still widely administered in the treatment of envenomation. Scorpion venoms is used as source of antigen to the production of antivenom. Therefore, quality control and homogeneity of venom are a crucial point. In this study, adult Mesobuthus gibbosus and Mesobuthus eupeus scorpions were collected from Osmaniye and Nigde provinces in Turkey. After extraction, the venom composition was analyzed using one-dimensional gel electrophoresis (1-DE). Only one common protein (70 kDa) band was found in all venom samples. Three protein bands (70, 87 and 100 kDa) were also detected in the venom of female scorpions in Osmaniye and Nigde provinces.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Ghalim N, El-Hafny B, Sebti F, Heikel J, Lazar N, Moustanir R, Benslimane A: Scorpion envenomation and sero terherapy in Morocco. Am J Trop Med Hyg, 62 (2): 277-283, 2000.
- Possani LD, Becerril B, Delepierre M, Tytgat J: Scorpion toxins specific for Na+-channels. Eur J Biochem, 264, 287-300, 1999.
- Pimenta AM, De Marco Almeida F, Delima ME, Martin Eauclaire ME, Bougis PE: Individual variability in Tityus serrulatus (Scorpiones, Buthidae) venom elicited by matrix-assisted laser desorption/ionization time-of-fl ight mass spectrometry. Rapid Commun Mass Spectrom, 17 (5): 418, 2003.
- World Health Organization: WHO Expert Groups on Requirements for Biological Substances. Requirements for immune sera of animal origin. Geneva: WHO Tech Report Series, 413, 45-59 [Appendix 2], 1969.
- Escoubas P, Corzo G, Whiteley BJ, Célérier ML, Nakajima T: Matrix- assisted laser desorption/ionization time-of-fl ight mass spectrometry and high-performance liquid chromatography study of quantitative and qualitative variation in tarantula spider venoms. Rapid Commun Mass Spectrom, 16 (5): 403-13, 2002.
- Ozkan O, Kat I: Mesobuthus eupeus scorpionism in Sanliurfa region of Turkey. J Venom Anim Toxins Incl Trop Dis, 11 (4): 479-491, 2005.
- Ozkan O, Adiguzel S, Kar S, Yakistiran S, Cesaretli Y, Karaer KZ: Determination of potency and paraspecific effects of Androctonus crassicauda (Olivier, 1807) antivenom against Mesobuthus gibbosus
- (Brulle, 1832) venom (Scorpiones: Buthidae). J Venom Anim Toxins Incl Trop Dis, 13 (2): 500-508, 2007.
- Ozkan O, Kar S, Güven E, Ergün G: Comparison of proteins, lethality and immunogenic compounds of Androctonus crassicauda (Olivier, 1807)
- (Scorpiones: Buthidae) venom obtained by diff erent methods. J Venom Anim Toxins Incl Trop Dis, 13 (4): 844-856, 2007.
- Ozkan O, Uzun R, Adıguzel S, Cesaretli Y, Ertek M: Evaluation of scorpion sting incidence in Turkey. J Venom Anim Toxins Incl Trop Dis, 14 (1): 128-140, 2008.
- Adiguzel S: In vivo and in vitro eff ects of scorpion venoms in Turkey: A mini-review. J Venom Anim Toxins incl Trop Dis, 16 (2): 198-211, 2010.
- Kovarik F: Review of European scorpions, with a key to species. Sekret, 6, 38-44, 1999.
- Ozkan O, Filazi A: The determination of acute lethal dose-50 (LD50) levels of venom in mice, obtained by diff erent methods from scorpions, Androctonus crassicauda (Olivier 1807). Acta Parasitol Turcica, 28 (1): 50-53,
- Layne E: Spectrophotometric and Turbidimetric Methods for Measuring Proteins. Meth Enzymol, 3, 447-455, 1957.
- Stoscheck CM: Quantitation of protein. Meth Enzymol, 182, 50-69, Laemmli K: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685, 1970.
- Candiano G, Bruschi M, Musante L, Santucci L, Ghiggeri GM, Carnemolla B, Orecchia P, Zardi L, Righetti PG: Blue silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis, 25, 1327-1333, 2004.
- Kovarík F, Fet V, Soleglad ME, Yağmur EA: Etudes on iurids, III. Revision of the genus Iurus Thorell, 1876 (Scorpiones: Iuridae), with a description of two new species from Turkey. Euscorpius, 95, 1-212, 2010.
- Yagmur EA, Koc H, Kunt KB: Description of a new species of Leiurus Ehrenberg, 1828 (Scorpiones: Buthidae) from Southeastern Turkey. Euscorpius. 85, 1-20, 2009.
- Yagmur EA: First record of Orthochirus Karsch, 1891 (Scorpiones, Buthidae) from Turkey. Journal of Anatolian Natural Sciences, 1 (1): 15-19,
- Fet V, Braunwalder ME: The scorpions (Arachnida: scorpiones) of the aegen area: Current problems in taxonomy and biogeography. Belg J Zool, (1): 15-20, 2000.
- Karatas A, Karatas A: First record of Mesobuthus eupeus (C.L. Koch, ) from central Anatolia (Scorpiones: Buthidae). In, Fet V, Selden PA (Eds): Scorpions 2001: In Memoriam Gray A, Polis. pp. 297-299, Burnham Beeches, Bucks, British Arachnological Society, 2001.
- Karatas A: Mesobuthus eupeus (C.L. Koch, 1839) (Scorpiones: Buthidae) in Anatolia. Euscorpius, 7, 1-7, 2003.
- Ozkan O, Carhan A: The neutralizing capacity of Androctonus crassicauda antivenom against Mesobuthus eupeus scorpion venom. Toxicon, 52, 375-379, 2008.
- El Ayeb M, Rochat H: Polymorphism and quantitative variations of toxins in the venom of the scorpion Androctonus australis hector. Toxicon, (5): 755-760, 1985.
- Keskin NA, Koc H: A study on venom proteins of Iurus dufoureius asiaticus Birula, 1903 (Scropiones: Iuridae). Acta Parasitol Turcica, 30 (1): 62, 2006.
- Abdel-Rahman MA, Omran MAA, Abdel-Nabi IM, Veda V, McVean A: Intraspecific variation in the Egyptian scorpion Scorpio maurus palmatus venom collected from diff erent biotopes. Toxicon, 53, 349-359, 2009.
- Badhe RV, Thimas AB, Harer SL, Desphande AD, Salvi N, Waghmare A: Intraspecific variation in protein of red scorpion (Mesobuthus tamulus, Coconsis, Pocock) venoms from Western and Southern India. J Venom Anim Toxins Incl Trop Dis, 12 (4): 612-619, 2006.
- El-Hafny B, Chgoury F, Adil N, Chen N, Nassar M: Intraspecific variability and pharmacokinetics, characteristics of Androctonus mauretanicus scorpion venom. Toxicon, 40 (11): 1609-1616, 2002.
- Kalapothakis E, Chavez-Olortegui C: Venom variability among several Tityus serrulatus specimens. Toxicon, 35 (10): 1523-1529, 1997.
- Omran MA, Mcvean A: Intraspecific variation in scorpion Leiurus quinquestriatus venom collected from Egypt (Sinai and Aswan deserts). J Toxicol Toxin Rev, 19 (3-4): 247-264, 2000.
- Ozkan O, Ciftci G: Individual variation in the protein profile of the venom of Mesobuthus gibbosus (Brullé, 1832, Scorpiones: Buthidae) from Turkey. J Venom Anim Toxins Incl Trop Dis, 16 (3): 505-508, 2010.
- Ucar G, Tas C: Cholinesterase inhibitory activities of the scorpion Mesobuthus gibbosus (Buthidae) venom peptides. FABAD J Pharm Sci, 28, 70, 2003.
- Bücherl, W: Classification, biology and venom extraction of scorpion In, Bücherl W, Buckley E (Eds): Venomous Animals and Their Venoms. Venomous Invertebrates. 3, 317-47 New York, Academic Press, 1971.
- Krifi MN, Marrakchi N, El Ayeb M, Dellagi K: Eff ect of some variables on the in vivo determination of scorpion and viper venom toxicities. Biologicals, 26, 277-288, 1998.
- Padilla A, Govezensky T, Possani LD, Larralde C: Mortality and antibody responses of mice to three successive episodes of experimental scorpion (Centruroides limpidus limpidus) envenomation and immunological recue. Toxicon, 46, 142-149, 2005.
- Calvete JJ: Antivenomics and venom phenotyping: A marriage of convenience to address the performance and range of clinical use of antivenoms. Toxicon, 56 (7): 1284-1291, 2010.
- Adigüzel S, Ozkan O, Inceoglu B: Epidemiological and clinical characteristic of scorpionism in children in Sanlıurfa. Toxicon, 49 (6): 875- , 2007.
| APA | ozkan o, ÇİFTCİ G, KARAER Z (2011). Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms. , 153 - 158. |
| Chicago | ozkan ozcan,ÇİFTCİ GÜLAY,KARAER Zafer Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms. (2011): 153 - 158. |
| MLA | ozkan ozcan,ÇİFTCİ GÜLAY,KARAER Zafer Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms. , 2011, ss.153 - 158. |
| AMA | ozkan o,ÇİFTCİ G,KARAER Z Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms. . 2011; 153 - 158. |
| Vancouver | ozkan o,ÇİFTCİ G,KARAER Z Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms. . 2011; 153 - 158. |
| IEEE | ozkan o,ÇİFTCİ G,KARAER Z "Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms." , ss.153 - 158, 2011. |
| ISNAD | ozkan, ozcan vd. "Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms". (2011), 153-158. |
| APA | ozkan o, ÇİFTCİ G, KARAER Z (2011). Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17((A)), 153 - 158. |
| Chicago | ozkan ozcan,ÇİFTCİ GÜLAY,KARAER Zafer Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 17, no.(A) (2011): 153 - 158. |
| MLA | ozkan ozcan,ÇİFTCİ GÜLAY,KARAER Zafer Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.17, no.(A), 2011, ss.153 - 158. |
| AMA | ozkan o,ÇİFTCİ G,KARAER Z Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2011; 17((A)): 153 - 158. |
| Vancouver | ozkan o,ÇİFTCİ G,KARAER Z Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2011; 17((A)): 153 - 158. |
| IEEE | ozkan o,ÇİFTCİ G,KARAER Z "Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17, ss.153 - 158, 2011. |
| ISNAD | ozkan, ozcan vd. "Electrophoretical Comparison of Proteins of Mesobuthus eupeus and Mesobuthus gibbosus Scorpion Venoms". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 17/(A) (2011), 153-158. |
