Yıl: 2017 Cilt: 10 Sayı: 1 Sayfa Aralığı: 18 - 30 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities

Öz:
Bu çalışmada, Anadolu'da yetişen üç endemik Astragalus türünün kimyasal içeriği ile bazı biyolojik aktivitelerinin belirlenmesi hedeflenmiştir. Astragalus leporinus var. hirsutus, Astragalus distinctissimus ve Astragalus schizopterus türlerinin kimyasal içeriği LC-MS/MS ve GC-MS ile karakterize edilmiştir. Biyolojik aktivite açısından; ekstrelerin antioksidan, antikolinesteraz, antimikrobiyal ve sitotoksik aktiviteleri tespit edilmiştir. Ek olarak, ekstrelerin ana bileşenlerinin de antioksidan özellikleri belirlenerek elde edilen sonuçlar ekstrelerin antioksidan kapasiteleri ile karşılaştırılmıştır. Çalışılan Astragalus türlerinde en bol bulunan flavonoidin rutin (1028,276-13351,76 µg/g ekstre) ve hesperidin (1604,348-9695,435 µg/g ekstre) olduğu, ayrıca A. schizopterus metanol ekstresinde de yüksek miktarda kuinik asit (111302,774 µg/g ekstre) bulunduğu belirlenmiştir. Palmitik asitin (C16:0), A. leporinus var. hirsutus (%32,9), A. distinctissimus (%32,5) ve A.schizopterus (%23,4)'da başlıca bileşik olduğu saptanmıştır. A. schizopterus metanol ekstresi lipit peroksidasyon (19,62±0,29), DPPH serbest radikal (54,61±0,38) , ABTS katyon radikali süpürücü aktivitesi (22,01±0,07), ve CUPRAC yönteminde en yüksek aktiviteyi göstermiştir. Çalışılan tüm ekstrelerden sadece A. leporinus var. hirsutus petrol eteri ekstresi orta derecede asetil- ve bütiril- kolinesteraz enzim inhibisyonu sergilemiştir.. Bitkilerin metanol ekstreleri C. albicans'a karşı orta derecede antimikrobiyal aktivite ortaya koymuşlardır. A. leporinus var. hirsutus metanol ekstresi, L929 fibroblast hücrelerine karşı en fazla canlılığı ve A549 hücrelerine karşı en yüksek sitotoksik etkiyi göstermiştir. Çalışmdan elde ettiğimiz bulgular ışığında, hayvan yemi olarak kullanılan bu Astragalus türleri, gıda ve ilaç endüstrisinde kullanılabilen doğal, biyolojik olarak aktif maddeler kaynağı olabilir.
Anahtar Kelime:

Konular: Biyoloji

Anadolu'daki üç endemik Astragalus türünün toplam fenolik-flavonoid içerikleri ve biyolojik aktivitelerine yönelik LC-MS / MS ve GC-MS analizleri

Öz:
Present work aims to determine the chemical profile and some biological activity of three endemic Astragalus species from Anatolia. The chemical contents of Astragalus leporinus var. hirsutus, Astragalus distinctissimus and Astragalus schizopterus were characterised by LC-MS/MS and GC-MS. In terms of biological activity; the antioxidant, anticholinesterase, antimicrobial and cytotoxic activities were determined. Additionally, the antioxidant properties of the major components were also determined and compared to the antioxidant capacities of these extracts. The most abundant flavonoids in these Astragalus species were determined as rutin (1028.276-13351.76 µg/g extract) and hesperidin (1604.348-9695.435 µg/g extract). A high amount of quinic acid (111302.774 µg/g extract) was detected in A. schizopterus methanol extract. Palmitic acid (C16:0) was found to be the major compound in A. leporinus var. hirsutus (32.9%), A. distinctissimus (32.5%), and A.schizopterus (23.4%). A. schizopterus methanol extract exhibited the highest antioxidant effect in lipid peroxidation (19.62±0.29), DPPH free (54.61±0.38) and ABTS cation radicals scavenging activity (22.01±0.07), and CUPRAC assays. Among all of the extracts, only A. leporinus var. hirsutus petroleum ether extract showed moderate inhibitory activity against acetyl- and butyryl-cholinesterase enzymes. The methanol extracts of the plants exhibited moderate activity against C. albicans. A. leporinus var. hirsutus methanol extract indicated the most viability against L929 fibroblast cells, and the highest cytotoxic effect against A549 cells. In consideration of our findings , these Astragalus species used as animal feed could be a source of naturally, biologically active agents that can be used in food and pharmaceutical industry
Anahtar Kelime:

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA HAŞİMİ N, ERTAŞ A, YILMAZ M, Boga M, temel h, DEMİRCİ KAYIRAN S, YILMAZ ÖZDEN T, YENER I, KOLAK U (2017). LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities. , 18 - 30.
Chicago HAŞİMİ NESRİN,ERTAŞ ABDULSELAM,YILMAZ Mustafa Abdullah,Boga Mehmet,temel hamdi,DEMİRCİ KAYIRAN SERPİL,YILMAZ ÖZDEN TUĞBA,YENER Ismail,KOLAK UFUK LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities. (2017): 18 - 30.
MLA HAŞİMİ NESRİN,ERTAŞ ABDULSELAM,YILMAZ Mustafa Abdullah,Boga Mehmet,temel hamdi,DEMİRCİ KAYIRAN SERPİL,YILMAZ ÖZDEN TUĞBA,YENER Ismail,KOLAK UFUK LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities. , 2017, ss.18 - 30.
AMA HAŞİMİ N,ERTAŞ A,YILMAZ M,Boga M,temel h,DEMİRCİ KAYIRAN S,YILMAZ ÖZDEN T,YENER I,KOLAK U LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities. . 2017; 18 - 30.
Vancouver HAŞİMİ N,ERTAŞ A,YILMAZ M,Boga M,temel h,DEMİRCİ KAYIRAN S,YILMAZ ÖZDEN T,YENER I,KOLAK U LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities. . 2017; 18 - 30.
IEEE HAŞİMİ N,ERTAŞ A,YILMAZ M,Boga M,temel h,DEMİRCİ KAYIRAN S,YILMAZ ÖZDEN T,YENER I,KOLAK U "LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities." , ss.18 - 30, 2017.
ISNAD HAŞİMİ, NESRİN vd. "LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities". (2017), 18-30.
APA HAŞİMİ N, ERTAŞ A, YILMAZ M, Boga M, temel h, DEMİRCİ KAYIRAN S, YILMAZ ÖZDEN T, YENER I, KOLAK U (2017). LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities. Biological Diversity and Conservation, 10(1), 18 - 30.
Chicago HAŞİMİ NESRİN,ERTAŞ ABDULSELAM,YILMAZ Mustafa Abdullah,Boga Mehmet,temel hamdi,DEMİRCİ KAYIRAN SERPİL,YILMAZ ÖZDEN TUĞBA,YENER Ismail,KOLAK UFUK LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities. Biological Diversity and Conservation 10, no.1 (2017): 18 - 30.
MLA HAŞİMİ NESRİN,ERTAŞ ABDULSELAM,YILMAZ Mustafa Abdullah,Boga Mehmet,temel hamdi,DEMİRCİ KAYIRAN SERPİL,YILMAZ ÖZDEN TUĞBA,YENER Ismail,KOLAK UFUK LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities. Biological Diversity and Conservation, vol.10, no.1, 2017, ss.18 - 30.
AMA HAŞİMİ N,ERTAŞ A,YILMAZ M,Boga M,temel h,DEMİRCİ KAYIRAN S,YILMAZ ÖZDEN T,YENER I,KOLAK U LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities. Biological Diversity and Conservation. 2017; 10(1): 18 - 30.
Vancouver HAŞİMİ N,ERTAŞ A,YILMAZ M,Boga M,temel h,DEMİRCİ KAYIRAN S,YILMAZ ÖZDEN T,YENER I,KOLAK U LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities. Biological Diversity and Conservation. 2017; 10(1): 18 - 30.
IEEE HAŞİMİ N,ERTAŞ A,YILMAZ M,Boga M,temel h,DEMİRCİ KAYIRAN S,YILMAZ ÖZDEN T,YENER I,KOLAK U "LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities." Biological Diversity and Conservation, 10, ss.18 - 30, 2017.
ISNAD HAŞİMİ, NESRİN vd. "LC-MS/MS and GC-MS analyses of three endemic Astragalus species from Anatolia towards their total phenolicflavonoid contents and biological activities". Biological Diversity and Conservation 10/1 (2017), 18-30.