Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella

Hülya ALTUNTAŞ, (Department of Biology, Faculty of Science, Anadolu University, Eskişehir, 26470, Turkey)
EMİNE DUMAN ERBAŞ (Department of Biology, Faculty of Science, Anadolu University, Eskişehir, 26470, Turkey)
Biological Diversity and Conservation
2 1
Yıl: 2017 Cilt: 10 Sayı: 1 Sayfa Aralığı: 153 - 159 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella

Öz:
Biyolojik, fizyolojik ve biyokimyasal araştırmalarda model organizma olarak büyük öneme sahip olan Galleria mellonella L. (Lepidoptera: Pyralidae) larvaları üzerinde entomopatojenik fungus Purpureocillium lilacinum (Thom) Luangsa-ard, Houbraken, Hywel-Jones ve Samson'un toksikolojik etkileri araştırılmıştır. Bu amaçla ortalama letal konsantrasyon (LC50) olan 2.82 x 103 spor/ml süspansiyonu kullanılarak son evre larvalara enjeksiyon yapıldı. Enjeksiyondan 24, 48, 72 ve 96 saat sonra larvalardan hemolenf alındı. Hemolenf örneklerinde toplam protein miktarı, katalaz (CAT) ve glutatyon S-transferaz (GST) aktiviteleri spektrofotometrik olarak analiz edildi. P. lilacinus ile enfekte olan larvaların toplam protein miktarı tüm saatlerde kontrol grupları ile karşılaştırıldığında azalış göstermektedir. Ayrıca fungal enfeksiyona bağlı olarak GST enzim aktivitesi zamanla artış gösterirken, CAT enzim aktivitesinde herhangi bir değişiklik olmadığı belirlenmiştir. Bu sonuçlar, larval GST aktivitesinde görülen artışın patojenite kaynaklı oksidatif stresin telafi edilebilmesi için fizyolojik bir uyum olabileceğine, CAT aktivitesinde hiçbir değişimin olmaması ise patojenite kaynaklı artan süperoksit radikallerinin enzimin inaktivasyonuna neden olabileceğine işaret etmektedir. Bu nedenle, CAT ve GST aktivitesinde görülen bu değişikliklerin nedeninin patojen kaynaklı strese karşı oluşan fizyolojik onarım mekanizmalarıyla ilişkili olduğunu düşünmekteyiz
Anahtar Kelime:

Konular: Biyoloji

Entomopatojenik Purpureocillium lilacinus’un model organizma, Galleria mellonella üzerindeki toksikolojik etkileri

Öz:
The toxicological effects of entomopathogenic fungus Purpureocillium lilacinum (Thom) Luangsa-ard, Houbraken, Hywel-Jones and Samson on the Galleria mellonella L. (Lepidoptera: Pyralidae) larvae which have great importance in biological, physiological and biochemical studies as a model insect were investigated. For this purpose, larvae were injected with median lethal concentration (LC50) 2.82 x 103 conidia/ml suspension. Hemolymph samples were collected after injection at 24, 48, 72 and 96 hours. Total protein analysis and specific enzyme activities of CAT and GST in the hemolymph were analyzed spectrophotometrically. Total protein amount in the hemolymph of the infected larvae decreased at all times when compared with control groups. Furthermore, the activity of GST in the hemolymph of the infected larvae increased at all times compared with untreated larvae, but the activity of CAT was similar with activity of untreated larvae. Consequently, increasing the activity of GST showed that entomopathogen organisms are effective on the antioxidant defense system of insects as a source of free radical. CAT activity in the absence of any change showed inactivation of CAT out of increased superoxide radicals based pathogenicity. Therefore, we suggest that the variance in the activity of CAT and GST in larvae may indicate a physiological adaptability to compensate for pathogen-induced stress
Anahtar Kelime:

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ALTUNTAS H, DUMAN ERBAŞ E (2017). Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella. , 153 - 159.
Chicago ALTUNTAS HULYA,DUMAN ERBAŞ EMİNE Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella. (2017): 153 - 159.
MLA ALTUNTAS HULYA,DUMAN ERBAŞ EMİNE Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella. , 2017, ss.153 - 159.
AMA ALTUNTAS H,DUMAN ERBAŞ E Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella. . 2017; 153 - 159.
Vancouver ALTUNTAS H,DUMAN ERBAŞ E Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella. . 2017; 153 - 159.
IEEE ALTUNTAS H,DUMAN ERBAŞ E "Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella." , ss.153 - 159, 2017.
ISNAD ALTUNTAS, HULYA - DUMAN ERBAŞ, EMİNE. "Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella". (2017), 153-159.
APA ALTUNTAS H, DUMAN ERBAŞ E (2017). Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella. Biological Diversity and Conservation, 10(1), 153 - 159.
Chicago ALTUNTAS HULYA,DUMAN ERBAŞ EMİNE Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella. Biological Diversity and Conservation 10, no.1 (2017): 153 - 159.
MLA ALTUNTAS HULYA,DUMAN ERBAŞ EMİNE Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella. Biological Diversity and Conservation, vol.10, no.1, 2017, ss.153 - 159.
AMA ALTUNTAS H,DUMAN ERBAŞ E Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella. Biological Diversity and Conservation. 2017; 10(1): 153 - 159.
Vancouver ALTUNTAS H,DUMAN ERBAŞ E Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella. Biological Diversity and Conservation. 2017; 10(1): 153 - 159.
IEEE ALTUNTAS H,DUMAN ERBAŞ E "Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella." Biological Diversity and Conservation, 10, ss.153 - 159, 2017.
ISNAD ALTUNTAS, HULYA - DUMAN ERBAŞ, EMİNE. "Toxicological effects of the entomopathogenic Purpureocillium lilacinus on the model organism, Galleria mellonella". Biological Diversity and Conservation 10/1 (2017), 153-159.
Sayfa Oluşturulma Tarihi
03-05-2024 9:52

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