Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA

Aybay, Cemalettin; Karakus, Resul

Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA

Cemalettin AYBAY, (Gazi Üniversitesi, Tıp Fakültesi, İmmünoloji Anabilim Dalı, Ankara, Türkiye)

Resul KARAKUŞ (Girilmedi)

Turkish Journal of Medical Sciences

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Yıl: 2003 Cilt: 33 Sayı: 1 Sayfa Aralığı: 1 - 5 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA

Öz:

Microalbuminuria is considered to be an early indicator of diabetic nephropathy. We have previously reported the establishment of A-HAlb/98 hybridoma secreting monoclonal antibody that specifically recognizes human serum albumin (HSA). In this report, an in-house enzyme-linked immunosorbent assay (ELISA) was developed using this antibody for the measurement of urinary albumin (UA) and UA was examined in 24 different samples. A-HAlb/98 was used as the capture antibody by immobilizing onto microtitre plates in ELISA format. HSA-specific polyclonal rabbit immunoglobulin was labelled with biotin and then used as the tracer antibody. The dynamic assay range of ELISA for albumin was established to be 10-1000 ng/ml, and the albumin levels determined by ELISA were well correlated (r = 0.8831) with those obtained by the liquid-phase immunoprecipitation method (The Orion Diagnostica TURBOX® U-albumin assay). The ability of this in-house ELISA to quantitate UA in nanogram ranges makes it a favourable candidate for utilization in diagnostic applications as well as in research studies.

Anahtar Kelime: Urine Antibodies, Monoclonal Microalbuminuria Enzyme-Linked Immunosorbent Assay Albumins

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Aybay C, Karakus R (2003). Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA. , 1 - 5.
Chicago	Aybay Cemalettin,Karakus Resul Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA. (2003): 1 - 5.
MLA	Aybay Cemalettin,Karakus Resul Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA. , 2003, ss.1 - 5.
AMA	Aybay C,Karakus R Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA. . 2003; 1 - 5.
Vancouver	Aybay C,Karakus R Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA. . 2003; 1 - 5.
IEEE	Aybay C,Karakus R "Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA." , ss.1 - 5, 2003.
ISNAD	Aybay, Cemalettin - Karakus, Resul. "Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA". (2003), 1-5.

APA	Aybay C, Karakus R (2003). Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA. Turkish Journal of Medical Sciences, 33(1), 1 - 5.
Chicago	Aybay Cemalettin,Karakus Resul Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA. Turkish Journal of Medical Sciences 33, no.1 (2003): 1 - 5.
MLA	Aybay Cemalettin,Karakus Resul Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA. Turkish Journal of Medical Sciences, vol.33, no.1, 2003, ss.1 - 5.
AMA	Aybay C,Karakus R Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA. Turkish Journal of Medical Sciences. 2003; 33(1): 1 - 5.
Vancouver	Aybay C,Karakus R Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA. Turkish Journal of Medical Sciences. 2003; 33(1): 1 - 5.
IEEE	Aybay C,Karakus R "Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA." Turkish Journal of Medical Sciences, 33, ss.1 - 5, 2003.
ISNAD	Aybay, Cemalettin - Karakus, Resul. "Measurement of urine albumin levels with a monoclonal antibody based in-house ELISA". Turkish Journal of Medical Sciences 33/1 (2003), 1-5.