Yıl: 2016 Cilt: 22 Sayı: 1 Sayfa Aralığı: 42 - 53 Metin Dili: Türkçe

Elicitor Applications to Cell Suspension Culture for Production of Phenolic Compounds in Grapevine

Öz:
Bu araştırmada asmada hücre süspansiyon kültürlerinde fenolik bileşiklerin üretiminde kadmiyum sülfat (CdSO4)floresan radyasyonu, metil jasmonat (MeJA) ve sukroz uygulamalarının etkileri incelenmiştir. Gamay, Kalecik karası veÖküzgözü üzüm çeşitlerine ait yaprak saplarından elde edilen kallus hücre süspansiyon kültürlerine CdSO4(0,1 ve 1.5 mM), MeJA (0 ve 10 µM) ve sukroz (0, 0.20 ve 0.25 M) uygulanmıştır. Floresan radyasyonu için hücresüspansiyonları tümüyle karanlıkta ya da 10000 lux ışık altında tutulmuştur. Toplam fenolik, toplam flavanol, toplamflavonol ve antosiyanin içerikleri spektrofotometrik olarak; trans-resveratrol içeriği ise HPLC ile belirlenmiştir.1.5 mM konsantrasyonundaki CdSO4ve 10 µM konsantrasyonundaki MeJA bütün çeşitlerde en yüksek fenolik bileşiküretimini sağlamıştır. Özellikle 1.5 mM CdSO4uygulanmış Kalecik karası en yüksek toplam fenolik madde (3.144 mgg-1), antosiyanin (1.672 CV g-1) ve trans-resveratrol (3.650 µg g-1) içeriğine sahip olmuştur. 10 µM konsantrasyonundakiMeJA uygulaması ise Öküzgözü çeşidinde trans-resveratrol miktarının 11.681 µg g-1 gibi yüksek bir değere çıkmasınısağlamıştır. Sukroz uygulamaları içinde 0.20 M dozu en yüksek toplam fenolik (4.215 mg g-1) ve trans-resveratrol(7.550 µg g-1) miktarını Kalecik karasında, en yüksek antosiyanin birikimini ise Gamay çeşidinde sağlamıştır. Karanlıkuygulaması bütün çeşitlerde trans-resveratrol birikimini kuvvetli bir şekilde artırmıştır. Toplam fenolik ve antosiyaninsentezinin ışık tarafından uyarıldığı belirlenmiştir. Sonuçlar CdSO4, MeJA, sukroz ve floresan radyasyonu gibi elisitöruygulamalarının üzümde fenolik bileşiklerin üretiminde önemli bir yaklaşım olabileceğini göstermiştir.
Anahtar Kelime:

Asmada Hücre Süspansiyon Kültürlerine Elisitör Uygulamaları ile Fenolik Bileşiklerin Üretilmesi

Öz:
In this study, the effects of cadmium sulphate (CdSO4), fleuresans irradiation, methyl jasmonate (MeJA) and sucrose treatments on the production of phenolic compounds in grapevine cell suspension cultures initiated from callus from petiole tissues of Vitis vinifera L. cvs. Gamay, Kalecik karası and Öküzgözü were investigated. As the elicitors of CdSO4(0, 1 and 1.5 mM), MeJA (0 and 10 µM) and sucrose (0, 0.20 and 0.25 M) were applied. Cell suspensions were exposed to visible light (10,000 lux) for fleuresans irradiation or cultured in dark constantly. Total phenolics, total flavanols, total flavonols and anthocyanin were determined spectrophotometrically while trans-resveratrol was quantified by HPLC. CdSO4 at 1.5 mM concentration and MeJA at 10 µM concentration yielded the highest phenolic productions in all cultivars. Especially, Kalecik Karası treated with CdSO4 at 1.5 mM had the highest total phenolic (3.144 mg g-1), anthocyanin (1.672 CV g-1) and trans-resveratrol (3.650 µg g-1) contents. MeJA application at 10 µM provided the transresveratrol accumulation as high as 11.681 µg g-1 in Öküzgözü. 0.20 M sucrose concentration resulted in the highest total phenolics (4.215 mg g-1) and trans-resveratrol (7.550 µg g-1) in Kalecik Karası cultures while the most anthocyanin accumulation (2.024 CV g-1) was achieved from Gamay. Darkness had strongly increased trans-resveratrol content in all cultivars, whereas total phenolics and anthocyanin synthesis were induced by light. Elicitor applications of CdSO4, MeJA, sucrose and fleuresans irradiation can be an efficient approach for the production of phenolics in grapevines
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ÇETİN E, GÖKTÜRK BAYDAR N (2016). Elicitor Applications to Cell Suspension Culture for Production of Phenolic Compounds in Grapevine. Tarım Bilimleri Dergisi, 22(1), 42 - 53.
Chicago ÇETİN EMİNE SEMA,GÖKTÜRK BAYDAR NİLGÜN Elicitor Applications to Cell Suspension Culture for Production of Phenolic Compounds in Grapevine. Tarım Bilimleri Dergisi 22, no.1 (2016): 42 - 53.
MLA ÇETİN EMİNE SEMA,GÖKTÜRK BAYDAR NİLGÜN Elicitor Applications to Cell Suspension Culture for Production of Phenolic Compounds in Grapevine. Tarım Bilimleri Dergisi, vol.22, no.1, 2016, ss.42 - 53.
AMA ÇETİN E,GÖKTÜRK BAYDAR N Elicitor Applications to Cell Suspension Culture for Production of Phenolic Compounds in Grapevine. Tarım Bilimleri Dergisi. 2016; 22(1): 42 - 53.
Vancouver ÇETİN E,GÖKTÜRK BAYDAR N Elicitor Applications to Cell Suspension Culture for Production of Phenolic Compounds in Grapevine. Tarım Bilimleri Dergisi. 2016; 22(1): 42 - 53.
IEEE ÇETİN E,GÖKTÜRK BAYDAR N "Elicitor Applications to Cell Suspension Culture for Production of Phenolic Compounds in Grapevine." Tarım Bilimleri Dergisi, 22, ss.42 - 53, 2016.
ISNAD ÇETİN, EMİNE SEMA - GÖKTÜRK BAYDAR, NİLGÜN. "Elicitor Applications to Cell Suspension Culture for Production of Phenolic Compounds in Grapevine". Tarım Bilimleri Dergisi 22/1 (2016), 42-53.