Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination

MOCAN KUZEY, Gamze; DEMİREZEN, Sayeste; USTAÇELEBİ, Şemsettin; BEKSAÇ, Mehmet Sinan; SAFİ ÖZ, ZEHRA

Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination

Zehra Safi ÖZ, (Zonguldak Karaelmas University, Faculty of Medicine, Department of Medical Biology, Zonguldak)

Sayeste DEMİREZEN, (Hacettepe University, Faculty of Science, Department of Biology, Ankara)

Gamze MOCAN KUZEY, (Hacettepe University, Faculty of Medicine, Department of Pathology, Ankara)

Mehmet Sinan BEKSAÇ, (Hacettepe University, Faculty of Medicine, Department of Obstetrics and Gynecology, Ankara)

Şemsettin USTAÇELEBİ (Hacettepe University, Faculty of Medicine, Department of Microbiology and Clinic Microbiology, Ankara)

GORM:Gynecology Obstetrics & Reproductive Medicine

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Yıl: 2009 Cilt: 15 Sayı: 2 Sayfa Aralığı: 200915294 - 200915299 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination

Öz:

AMAÇ: Rutin jinekolojik incelemede Human Papilloma Virus(HPV) sıklığını belirlemek ve bu virüs ile hücresel değişiklikler,sitolojik ve jinekolojik bulgular arasında ilişki olup olmadığınıdeğerlendirmek.GEREÇ VE YÖNTEM: Rutin jinekolojik muayeneleri için Ji ne -koloji kliniğine başvuran dörtyüz hasta çalışma kapsamınaalınmıştır. Bu hastalara ait konvansiyonel serviko-vajinal yaymalarPapanicolaou yöntemine göre boyanmış ve sitolojik olarakincelenmiştir. HPV DNA Polimeraz Zincir Reaksiyonu(PCR) ile değerlendirilmiştir. HPV pozitif örnekler ise yine aynıyöntemle HPV tip 16 ve 18 açısından incelenmiştir.BULGULAR: HPV DNA 400 hastanın 11 (%2.75)'inde saptan -mış tır. HPV pozitif hastalar HPV 16 ve 18 açısından PCR iledeğerlendirildiğinde ise 2 hastada HPV 16 ve 18 birlikte bulunurken1 hastada sadece HPV 18 saptanmıştır. HPV ile çekirdekzar düzensizliği, hiperkromatizm, koilositotik hücre, vakuolizasyonve post partum dönemde bulunma arasında anlamlıilişki saptanmıştır (p<0.05).SONUÇLAR: Pap simir incelemesinin HPV'a ait hücreseldeğişikliklerin tesbiti için değer taşıdığı ve eğer simir taramasındaşüphelenilirse hastanın HPV DNA taramasına yönlendirilmesininmaliyet açısından da yararlı olacağı düşüncesindeyiz.

Anahtar Kelime:

Konular: Kadın Hastalıkları ve Doğum

Human Papilloma Virüsün Rutin Jinekolojik Kontrole Gelen Kadınlarda Sitolojik ve Klinik Olarak Değerlendirilmesi

Öz:

OBJECTIVE: To determine the frequency of Human Papillomavirus infection in routine gynecologic examination and to detect whether there is a relationship between the presence of Human Papilloma Virus and cytologic and gynecological findings. STUDY DESIGN: Four hundred women applying to Gynecology outpatient clinic for routine gynecologic examination were included. Conventional Pap smears of 400 patients were stained by Papanicolaou technique and examined cytologically. HPV DNA was detected by in-house PCR . Also HPV positive samples were evaluated for HPV 16 and 18 by same method. RESULTS: Eleven (2.75%) of 400 women were HPV DNA (+). Two of which were type 16 and 18 and one of which was type 18. Nuclear membrane irregularity, hyperchromatism, koilocytotic cell, vacuolisation and post partum period corralated with HPV infection (p <0.05). CONCLUSION: Pap smear examination is a value of detection of cellular changes belonging Human Papillomavirus and if in this examination it is need to, these patients refer to Human Papillomavirus DNA screening it would be more cost effective.

Anahtar Kelime:

Konular: Kadın Hastalıkları ve Doğum

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SAFİ ÖZ Z, DEMİREZEN S, MOCAN KUZEY G, BEKSAÇ M, USTAÇELEBİ Ş (2009). Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination. , 200915294 - 200915299.
Chicago	SAFİ ÖZ ZEHRA,DEMİREZEN Sayeste,MOCAN KUZEY Gamze,BEKSAÇ Mehmet Sinan,USTAÇELEBİ Şemsettin Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination. (2009): 200915294 - 200915299.
MLA	SAFİ ÖZ ZEHRA,DEMİREZEN Sayeste,MOCAN KUZEY Gamze,BEKSAÇ Mehmet Sinan,USTAÇELEBİ Şemsettin Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination. , 2009, ss.200915294 - 200915299.
AMA	SAFİ ÖZ Z,DEMİREZEN S,MOCAN KUZEY G,BEKSAÇ M,USTAÇELEBİ Ş Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination. . 2009; 200915294 - 200915299.
Vancouver	SAFİ ÖZ Z,DEMİREZEN S,MOCAN KUZEY G,BEKSAÇ M,USTAÇELEBİ Ş Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination. . 2009; 200915294 - 200915299.
IEEE	SAFİ ÖZ Z,DEMİREZEN S,MOCAN KUZEY G,BEKSAÇ M,USTAÇELEBİ Ş "Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination." , ss.200915294 - 200915299, 2009.
ISNAD	SAFİ ÖZ, ZEHRA vd. "Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination". (2009), 200915294-200915299.

APA	SAFİ ÖZ Z, DEMİREZEN S, MOCAN KUZEY G, BEKSAÇ M, USTAÇELEBİ Ş (2009). Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination. GORM:Gynecology Obstetrics & Reproductive Medicine, 15(2), 200915294 - 200915299.
Chicago	SAFİ ÖZ ZEHRA,DEMİREZEN Sayeste,MOCAN KUZEY Gamze,BEKSAÇ Mehmet Sinan,USTAÇELEBİ Şemsettin Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination. GORM:Gynecology Obstetrics & Reproductive Medicine 15, no.2 (2009): 200915294 - 200915299.
MLA	SAFİ ÖZ ZEHRA,DEMİREZEN Sayeste,MOCAN KUZEY Gamze,BEKSAÇ Mehmet Sinan,USTAÇELEBİ Şemsettin Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination. GORM:Gynecology Obstetrics & Reproductive Medicine, vol.15, no.2, 2009, ss.200915294 - 200915299.
AMA	SAFİ ÖZ Z,DEMİREZEN S,MOCAN KUZEY G,BEKSAÇ M,USTAÇELEBİ Ş Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination. GORM:Gynecology Obstetrics & Reproductive Medicine. 2009; 15(2): 200915294 - 200915299.
Vancouver	SAFİ ÖZ Z,DEMİREZEN S,MOCAN KUZEY G,BEKSAÇ M,USTAÇELEBİ Ş Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination. GORM:Gynecology Obstetrics & Reproductive Medicine. 2009; 15(2): 200915294 - 200915299.
IEEE	SAFİ ÖZ Z,DEMİREZEN S,MOCAN KUZEY G,BEKSAÇ M,USTAÇELEBİ Ş "Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination." GORM:Gynecology Obstetrics & Reproductive Medicine, 15, ss.200915294 - 200915299, 2009.
ISNAD	SAFİ ÖZ, ZEHRA vd. "Cytologic and Clinical Evaluation of Human Papillomavirus in Women Underwent Routine Gynecologic Examination". GORM:Gynecology Obstetrics & Reproductive Medicine 15/2 (2009), 200915294-200915299.