A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract

Cevik, Ozge; ERUYGUR, Nuraniye; ATAŞ, Mehmet; Çetin, Süleyman

A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract

Nuraniye ERUYGUR, (Department of Pharmacognosy, Cumhuriyet University Faculty of Pharmacy Sivas; Turkey)

Süleyman ÇETİN, (Department of Pharmacognosy, Cumhuriyet University Faculty of Pharmacy Sivas; Turkey)

Mehmet ATAŞ, (Department of Pharmaceutical Microbiology, Cumhuriyet University Faculty of Pharmacy Sivas; Turkey)

Özge ÇEVİK (Department of Biochemistry, Adnan Menderes University School of Medicine Aydin; Turkey)

Cumhuriyet Tıp Dergisi (ELEKTRONİK)

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Yıl: 2017 Cilt: 39 Sayı: 3 Sayfa Aralığı: 531 - 538 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract

Öz:

Amaç: Tymbra türleri halk arasında antioxidant, antiseptic, antitussive, karminatif, antienflamatuvar ve antimikrobiyal özelliklerinden dolayı salata ve bitkisel çay olarak yaygın kullanılmaktadır. Çalışmamızın amacı, Thymbra spicata L. var. spicata.'dan hazırlanan ham etanol özütünün antioksidan, antimikrobiyal ve sitotoksik etkisini araştırmaktır. Yöntem: T.spicata L.var. spicata etanol ekstresinin antioksidan aktivitesi DPPH ve ABTS serbest radikal süpürücü etki, total fenol ve total flavonoid miktar tayini yöntemleri kullanılarak değerlendirildi. Antimikrobiyal aktivitesi ise mikrodilüsyon metodu ile, sitotoksisite aktivitesi prostat kanseri PC3 hücre hattı kullanılarak MTT metoduyla değerlendirildi. Bulgular: Total fenol ve total flavonoit miktarı kuru ekstre bazında 216.75 ± 0.017 (gallik asite eşdeğer mg/g) ve 78.17 ± 0.063 (kersetine eşdeğer mg/g) olarak bulundu. DPPH ve ABTS radikal süpürücü etki IC50 değeri sırasıyla 16.91 µg/mL and 5.2 ± 0.009 µg/mL olarak bulundu. Etanol ekstresinin test edilen mikroorganizmalar karşı minimum inhibe edici konsantrasonu 1.25-2.5 mg/mL aralığında tespit edildi. Sitotoksik aktivitenin değerlendirilmesi için MTT testi kullanıldı, etanol ekstraktı MCF-7 meme kanseri hücrelerine karşı IC50 değeri 340 ?g / mL olan büyümeyi önleyici bir etki gösterdi. Sonuç: Elde edilen bulgulara göre, Thymbra spicata L.var. spicata bitkisinin antioxidant, antimikrobiyal ve sitotoksik aktivitesi bu bitkinin halk arasındakı yaygın kullanımını bilimsel yönden desteklemektedir.

Anahtar Kelime:

Konular: Cerrahi

Thymbra spicata L. var. spicata etanol ekstresinin antioksidan, antimikrobiyal ve sitotoksik etkisinin araştırılması

Öz:

Objective: Thymbra species are widely used in Turkish folk medicine as salad or herbal tea for antioxidant, antiseptic, antitussive, carminative, anti-inflammatory and antimicrobial activities. The present study was aimed to determine antioxidant, antimicrobial and cytotoxic properties of ethanol crude extract prepared from Thymbra spicata L.var. spicata. Method: The antioxidant properties of ethanol extract of Thymbra spicata L. var. spicata was investigated using 1,1diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azinobis[3-ethylbenzthiazoline]-6-sulfonic acid (ABTS) radical scavenging activity, total flavonoid and total phenolic content analysis. Antimicrobial activity of the plant extract were determined using the microdilution method, while proliferative activity were evaluated by MTT assay on MCF-7 breast cancer and PC3 prostate cancer cell line. Results: Total phenol and total flavonoid content were 216.75 ± 0.017 (equivalent of Gallic acid mg/g dry extract) and 78.17 ± 0.063 (equivalent of Quercetin mg/g), IC50 value for DPPH and ABTS scavenging activity was 16.91 µg/mL and 5.2 ± 0.009 µg/mL, respectively. Antimicrobial activity observed for the extract ranging between 1.25 and 2.5 mg/mL as MIC value against tested microorganism. MTT assay was employed for evaluation of cytotoxic activity, ethanol extract demonstrated growth inhibitory effect against MCF-7 breast cancer cells with IC50 value 340 µg/mL. Conclusions: According to the obtained results, antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L.var. spicata is scientifically support the widespread use of this plant among the people.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ERUYGUR N, Çetin S, ATAŞ M, Cevik O (2017). A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract. , 531 - 538.
Chicago	ERUYGUR Nuraniye,Çetin Süleyman,ATAŞ Mehmet,Cevik Ozge A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract. (2017): 531 - 538.
MLA	ERUYGUR Nuraniye,Çetin Süleyman,ATAŞ Mehmet,Cevik Ozge A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract. , 2017, ss.531 - 538.
AMA	ERUYGUR N,Çetin S,ATAŞ M,Cevik O A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract. . 2017; 531 - 538.
Vancouver	ERUYGUR N,Çetin S,ATAŞ M,Cevik O A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract. . 2017; 531 - 538.
IEEE	ERUYGUR N,Çetin S,ATAŞ M,Cevik O "A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract." , ss.531 - 538, 2017.
ISNAD	ERUYGUR, Nuraniye vd. "A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract". (2017), 531-538.

APA	ERUYGUR N, Çetin S, ATAŞ M, Cevik O (2017). A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract. Cumhuriyet Tıp Dergisi (ELEKTRONİK), 39(3), 531 - 538.
Chicago	ERUYGUR Nuraniye,Çetin Süleyman,ATAŞ Mehmet,Cevik Ozge A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract. Cumhuriyet Tıp Dergisi (ELEKTRONİK) 39, no.3 (2017): 531 - 538.
MLA	ERUYGUR Nuraniye,Çetin Süleyman,ATAŞ Mehmet,Cevik Ozge A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract. Cumhuriyet Tıp Dergisi (ELEKTRONİK), vol.39, no.3, 2017, ss.531 - 538.
AMA	ERUYGUR N,Çetin S,ATAŞ M,Cevik O A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract. Cumhuriyet Tıp Dergisi (ELEKTRONİK). 2017; 39(3): 531 - 538.
Vancouver	ERUYGUR N,Çetin S,ATAŞ M,Cevik O A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract. Cumhuriyet Tıp Dergisi (ELEKTRONİK). 2017; 39(3): 531 - 538.
IEEE	ERUYGUR N,Çetin S,ATAŞ M,Cevik O "A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract." Cumhuriyet Tıp Dergisi (ELEKTRONİK), 39, ss.531 - 538, 2017.
ISNAD	ERUYGUR, Nuraniye vd. "A study on the antioxidant, antimicrobial and cytotoxic activity of Thymbra spicata L. var. spicata ethanol extract". Cumhuriyet Tıp Dergisi (ELEKTRONİK) 39/3 (2017), 531-538.