Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi

REHMAN, Asad ur; HAQ, Ikram Ul; RASOOL, Sunniya; MUKHTAR, Hamid

Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi

Asad ur REHMAN, (Institute Of Industrial Biotechnology, Government College University, Lahore, Pakistan)

Sunniya RASOOL, (Institute Of Industrial Biotechnology, Government College University, Lahore, Pakistan)

Hamid MUKHTAR, (Institute Of Industrial Biotechnology, Government College University, Lahore, Pakistan)

Ikram Ul HAQ (Institute Of Industrial Biotechnology, Government College University, Lahore, Pakistan)

Türk Biyokimya Dergisi

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Yıl: 2014 Cilt: 39 Sayı: 2 Sayfa Aralığı: 140 - 149 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi

Öz:

Amaç: Ekstraselüler lipazın katı faz fermantasyon ile mikrobiyal olarak üretilebilmesi için çeşitli maya kültürleri ve farklı tarım sanayi yan ürünleri değerlendirilerek fermantasyon koşullarının optimizasyonuGereç ve Yöntemler: Candida lipolytica NRRL-Y-1095, Candida utilis NRRL-Y-900, Candida tropicalis NRRL-Y-1552, Saccharomyces cerevisiae IIB-1 dahil bir çok maya kültürü ile tarım sanayi yan ürünleri katı faz fermantasyonu kullanılarak 250 mL erlenmayer içinde seri kültür ile taranmıştır. Vasatlar organik ve inorganik olmak üzere farklı azot kaynakları ve metal iyonları ile beslenmiştir.Bulgular: En iyi enzim üretimini Soya küspesi ile Candida utilis NRRL-Y-900 gösterdi. Koşullar sübstrat olarak değişik parçacık boyutları ve nemlendirici faktörler kullanılarak en fazla lipaz sentezi için optimize edilmiştir. Candida utilis NRRL-Y-900 ile enzim birikimi için optimum sıcaklık 30ºC, pH 6.5 dır. Yirmidört saatlik maya kültüründen %7.5 inoculum kullanıldığında 60 saatlik fermantasyon maksimum enzim üretimi sağlamıştır. Optimum vasat kompozisyonu 2% (w/v) et özü, 0.4% (w/v) amonyum sülfat ve 5mM Fe+2 içermektedir. Maksimum ekstracelüler lipaz üretimi 3.96±0.09 U olmuştur. Sonuçlar: Bildiğimiz kadarı ile bu çalışmanın soya küspesinden C. utilis NRRL-Y-900 ile optimize edilmiş katı faz fermantasyonu koşulları altında lipaz akümülasyonu gösteren ilk çalışma olması sebebi ile önemlidir

Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji

Production of an extracellular lipase by Candida utilis NRRL-Y-900 using agro-industrial by-products

Öz:

Aim:To screen various yeast cultures and agro-industrial by-products and optimization of fermentation conditions for the microbial production of extracellular lipase under solid state fermentation technique. Material and Methods: Various yeast cultures including Candida lipolytica NRRL-Y-1095, Candida utilis NRRL-Y-900, Candida tropicalis NRRL-Y-1552, Saccharomyces cerevisiae IIB-1 were screened by culturing on agro-industrial by-products under batch culture using solid state fermentation in 250 mL Erlenmeyer flasks. The medium was supplemented with various nitrogen sources (both organic and inorganic) and metal ions.Results: Candida utilis NRRL-Y-900 showed the highest enzyme production on soybean meal. Various particle sizes of substrate and moistening agents were also optimized for the maximum lipase synthesis. The optimum temperature and pH for the accumulation of enzyme by Candida utilis NRRL-Y-900 was 30ºC and 6.5, respectively. The fermentation time of 60h was suitable for the maximum enzyme production by using 7.5% inoculum of 24h old yeast culture. The optimal medium composition consisted of 2% (w/v) meat extract, 0.4% (w/v) ammonium sulphate and 5mM Fe+2. The maximum extracellular lipase production was 3.96±0.09 U. Conclusion: The results obtained during the study are significant for, to our knowledge, it is the first report regarding the utilization of soybean meal by C. utilis NRRL-Y-900 to accumulate lipase under optimized conditions and solid state fermentation

Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	REHMAN A, RASOOL S, MUKHTAR H, HAQ I (2014). Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi. , 140 - 149.
Chicago	REHMAN Asad ur,RASOOL Sunniya,MUKHTAR Hamid,HAQ Ikram Ul Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi. (2014): 140 - 149.
MLA	REHMAN Asad ur,RASOOL Sunniya,MUKHTAR Hamid,HAQ Ikram Ul Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi. , 2014, ss.140 - 149.
AMA	REHMAN A,RASOOL S,MUKHTAR H,HAQ I Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi. . 2014; 140 - 149.
Vancouver	REHMAN A,RASOOL S,MUKHTAR H,HAQ I Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi. . 2014; 140 - 149.
IEEE	REHMAN A,RASOOL S,MUKHTAR H,HAQ I "Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi." , ss.140 - 149, 2014.
ISNAD	REHMAN, Asad ur vd. "Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi". (2014), 140-149.

APA	REHMAN A, RASOOL S, MUKHTAR H, HAQ I (2014). Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi. Türk Biyokimya Dergisi, 39(2), 140 - 149.
Chicago	REHMAN Asad ur,RASOOL Sunniya,MUKHTAR Hamid,HAQ Ikram Ul Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi. Türk Biyokimya Dergisi 39, no.2 (2014): 140 - 149.
MLA	REHMAN Asad ur,RASOOL Sunniya,MUKHTAR Hamid,HAQ Ikram Ul Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi. Türk Biyokimya Dergisi, vol.39, no.2, 2014, ss.140 - 149.
AMA	REHMAN A,RASOOL S,MUKHTAR H,HAQ I Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi. Türk Biyokimya Dergisi. 2014; 39(2): 140 - 149.
Vancouver	REHMAN A,RASOOL S,MUKHTAR H,HAQ I Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi. Türk Biyokimya Dergisi. 2014; 39(2): 140 - 149.
IEEE	REHMAN A,RASOOL S,MUKHTAR H,HAQ I "Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi." Türk Biyokimya Dergisi, 39, ss.140 - 149, 2014.
ISNAD	REHMAN, Asad ur vd. "Tarım sanayi yan ürünlerinden Candida utilisNRRL-Y-900 ile ekstraselüler lipaz üretimi". Türk Biyokimya Dergisi 39/2 (2014), 140-149.