Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls

SOZEN, MUSTAFA MERT; KARTALCI, ŞÜKRÜ

Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls

Mustafa Mert SÖZEN, (İnonu University, Department of Molecular Biology and Genetics, Malatya, Turkey)

Şükrü KARTALCI (İnonu University Faculty of Medicine, Department of Psychiatry, Malatya, Turkey)

Türk Biyokimya Dergisi

9 1

Yıl: 2015 Cilt: 40 Sayı: 6 Sayfa Aralığı: 463 - 471 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls

Öz:

Amaç: İnsan ERBB4 geni (v-erb-a erithroblastic leukemia viral oncogene homology 4) ErbB4 reseptör proteinini kodlamaktadır. ErbB4 reseptörünün ligandlarından biri Neuregulin-1 olup şizofreni için yatkınlık geni olduğu rapor edilen NRG1 geni ile kodlanır. ErbB4 reseptörünün Neuregulin-1 bağlanması ile aktive olmasından dolayı, ERBB4 geninin de şizofreninin (SZ) ortaya çıkmasında rolünün olduğu düşünülmektedir. Bazı bağlantı (association), ifade ve hayvan çalışmalarının bu düşünceyi desteklemesine ve hastalık ile ERBB4 genindeki bazı tek nükleotid polimorfizmlerinin (SNP) bağlantılı olduğu göstermesine rağmen, bunlardan bağımsız olarak başka populasyonlarda yapılan çalışmaların sonuçları aynı yönde olmamıştır. Bu çalışmada biz de bazı ERBB4 SNP'lerinin bir Türk hasta grubunda şizofreninin ortaya çıkmasındaki rolü konusunda veriler elde etmeyi amaçladık. Metod: Bu çalışmada Malatya yöresinden toplanan bir Türk şizofreni hasta grubunda ve uygun bir kontrol grubunda yukarıda belirtilen bağlantının olup olmadığını test etmek amacı ile belirtilen 3 SNP (rs707284, rs7598440ve rs839523) taranmıştır. SNP genotiplenmesi için gerçek zamanlı PCR temelli bir yöntem kullanılmış, sonuçlar ki-kare testi ile değerlendirilmiştir. Her bir SNP'nin ayrı ayrı tek SNP bağlantısının yanısıra bu üç SNP'in oluşturduğu haplotipler de değerlendirilerek çoklu lokus bağlantıları da analiz edilmiştir. Bulgular: SNP genotipleme sonrasında yapılan hasta ve kontrol gruplarının allel, genotip ve haplotip dağılımları bakımından karşılaştırıldığı istatistiksel testlere göre, sözü edilen iki grup arasında taranan üç SNP bakımından önemli bir farklılık görülememiştir. Sonuç: Hastalarımızda ERBB4 geninde yer alan üç SNP ile şizofreni arasında bir bağlantının olduğuna dair anlamlı bir delil elde edilememiştir.

Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji

ERBB4 genindeki üç polimorfizmin bir Türk şizofreni hasta grubunda ve kontrollerde taranması

Öz:

Objective: The human ERBB4 gene (v-erb-a erithroblastic leukemia viral oncogene homology 4) codes for the ErbB4 receptor protein. One of the ligands of ErbB4 receptor is Neuregulin-1 and it is coded by NRG1 gene which is reported to be a susceptibility gene for schizophrenia. Since ErbB4 receptor is activated by binding of Neuregulin-1, ERBB4 gene is thought to be involved in development of schizophrenia (SZ) as well. Even though several association, expression and animal studies supported this idea and resulted with association between the disease and several single nucleotide polymorphisms (SNP) in the ERBB4 gene, independent studies done in different populations did not replicate that result. In the present study we aimed to get clues about involvement of three ERBB4 SNPs -which were found to be associated with schizophrenia in some other populations- in development of schizophrenia in a group of Turkish patients. Methods: We screened 3 SNPs (rs707284, rs7598440 and rs839523) in a group of Turkish schizophrenia patients and a matched control group from Malatya-Turkey to test the presence of such an association. For SNP genotyping, we used a real-time PCR based method and we evaluated the results with chi-square test. We have analyzed the haplotypes constituted by those three SNPs for multiple locus associations as well as analyzing each for single SNP associations. Results: After SNP genotyping and statistical tests comparing our case and control groups for allele, genotype and haplotype distributions, there was no significant difference between those two groups for three SNPs we screened. Conclusion: We were not able to find a significant evidence for presence of an association between three SNPs located in the ERBB4 gene and schizophrenia in our patients.

Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SOZEN M, KARTALCI Ş (2015). Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls. , 463 - 471.
Chicago	SOZEN MUSTAFA MERT,KARTALCI ŞÜKRÜ Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls. (2015): 463 - 471.
MLA	SOZEN MUSTAFA MERT,KARTALCI ŞÜKRÜ Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls. , 2015, ss.463 - 471.
AMA	SOZEN M,KARTALCI Ş Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls. . 2015; 463 - 471.
Vancouver	SOZEN M,KARTALCI Ş Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls. . 2015; 463 - 471.
IEEE	SOZEN M,KARTALCI Ş "Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls." , ss.463 - 471, 2015.
ISNAD	SOZEN, MUSTAFA MERT - KARTALCI, ŞÜKRÜ. "Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls". (2015), 463-471.

APA	SOZEN M, KARTALCI Ş (2015). Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls. Türk Biyokimya Dergisi, 40(6), 463 - 471.
Chicago	SOZEN MUSTAFA MERT,KARTALCI ŞÜKRÜ Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls. Türk Biyokimya Dergisi 40, no.6 (2015): 463 - 471.
MLA	SOZEN MUSTAFA MERT,KARTALCI ŞÜKRÜ Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls. Türk Biyokimya Dergisi, vol.40, no.6, 2015, ss.463 - 471.
AMA	SOZEN M,KARTALCI Ş Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls. Türk Biyokimya Dergisi. 2015; 40(6): 463 - 471.
Vancouver	SOZEN M,KARTALCI Ş Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls. Türk Biyokimya Dergisi. 2015; 40(6): 463 - 471.
IEEE	SOZEN M,KARTALCI Ş "Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls." Türk Biyokimya Dergisi, 40, ss.463 - 471, 2015.
ISNAD	SOZEN, MUSTAFA MERT - KARTALCI, ŞÜKRÜ. "Screening of three ERBB4 gene polymorphisms in a group of Turkish schizophrenia patients and controls". Türk Biyokimya Dergisi 40/6 (2015), 463-471.