Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi

ATASEVER, Ayhan; YAMAN, Duygu

Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi

Duygu YAMAN, (Erciyes Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Kayseri-TÜRKİYE)

Ayhan ATASEVER (Erciyes Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Kayseri-TÜRKİYE)

Erciyes Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2016 Cilt: 13 Sayı: 2 Sayfa Aralığı: 83 - 100 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi

Öz:

Bu çalışmada, karbon tetraklorür (CCl4) ile akut ve kronik karaciğer hasarı oluşturulan ratlarda biberiye ekstraktının (BE) histolojik yapı, bazı biyokimyasal parametreler ile lipid peroksidasyonu üzerine koruyucu etkileri belirlendi. Akut ve kronik dönemlerde, 40'ar adet (200-250 gr) Wistar albino ırkı rat, her birinde 10 hayvan olacak şekilde 4'er gruba ayrıldı. Akut çalışmada, birinci gruba (kontrol grubu) %0.9 NaCl (1.0 mL/kg), ikinci gruba 200mg/kg BE, üçüncü gruba CCl (1.0 mL/kg) sadece ilk haftada iki kere, dördüncü gruba, ilk hafta iki kez CCl ile eş zamanlı 200 mg/kg BE dört hafta verildi. Kronik çalışmada, birinci gruba (kontrol grubu) %0.9 NaCl (0,2 mL/kg), ikinci gruba 200 mg/kg BE, üçüncü gruba haftada iki kere 0.2 mL/kg CCl, dördüncü gruba, haftada iki kere 0.2 mL/kg CCl4 ile eş zamanlı 200 mg/kg BE on iki hafta verildi. Akut ve kronik kontrol ve BE gruplarında normal doku yapısı gözlenirken; akut CCl uygulananların hepatositlerinde yoğun makro ve mikroveziküler yağlanma, portal bölgede ve parankimde mononüklear hücre infiltrasyon alanları ile nekrotik değişiklikler görüldü. Kronik CCl4 grubunda, akut bulgulara ilaveten portal bölgelerde ortadan şiddetliye ulaşan fibrozis ile lobulasyon formasyonları görüldü. Akut ve kronik karaciğer hasarı oluşturulan gruplara BE ilavesi histopatolojik lezyonları etkilemedi. Karbon tetraklorür uygulaması ile tüm biyokimyasal parametreler (AST, ALT, ALP, glikoz, trigliserid, total kolesterol, HDL-kolesterol, LDL-kolesterol, total protein, albümin) değişti. Biberiye ekstraktı, akut dönemde düşen HDL-kolesterol düzeylerini artırırken, diğer parametrelerle, kronik dönemde de tüm parametreleri etkilemedi. Akut ve kronik uygulanan CCl, karaciğer MDA ve NO değerlerini arttırdı. Biberiye ekstraktı MDA ve NO değerlerini değiştirmedi.Sonuç olarak; CCl ile oluşturulan akut ve kronik karaciğer hasarı üzerine 200 mg/kg BE ilavesinin herhangi bir etkisi görülmedi. Biberiye ekstraktının farklı sürelerde ve yan etki oluşturmaksızın en iyi sonucu verecek farklı dozlarda kullanılarak, etkilerinin belirlenmesine yönelik yeni araştırmalara ihtiyaç olduğu kanaatine varıldı.

Anahtar Kelime:

Konular: Tıbbi Araştırmalar Deneysel Genel ve Dahili Tıp Pediatri

The Effects of Rosemary Extract (Rosmarinus officinalis) on Carbon Tetrachloride Induced Acute and Chronic Hepatic Damage

Öz:

The present study evaluated protective role of rosemary extract (RE) on histological structure, some serum biochemical parameters and lipid peroxidation on chronic liver injury induced by carbon tetrachloride (CCl) in rats. In acute and chronic period 40 Wistar-albino rats (200-250g) were divided into four groups of ten, for per period. In the acute study, the rats in the first group (control group), were administered with %0.9 NaCl (1mL/kg); second group was administered with 200mg/kg RE, third group was administered with CCl4 (1mL/kg) twice in the first week, fourth group was administered with CCl(1mL/kg) twice in the first week and simultaneously for 4 weeks 200mg/kg RE. In the chronic study, the rats in the first group (control group), were administered with %0.9 NaCl (0.2mL/kg); second group was administered with 200mg/kg RE, third group was administered with CCl(0.2mL/kg) twice for 12 weeks, fourth group was administered with CCl4 (0.2mL/kg) twice for 12 weeks and simultaneously with 200mg/kg RE. In case of acute and chronic control and RE group livers had normal architecture in the acute CCltreated group, an intensive macro and microvesicular steatosis, mononuclear inflammatory cell infiltrations in portal area and parenchyma and necrotic alterations; in the chronic group additionally to acute findings mild to severe fibrosis with lobulation formation were observed. In acute and chronic groups RE administration did not affect the histopathological lesions. Administration of CCl4, changed all biochemical parameters (AST, ALT, ALP, glucose, triglyceride, total cholesterol, HDL-cholesterol, LDL-cholesterol, total protein, albumin). In the acute and chronic periods of study, RE administration didn't have any effect on biochemical parameters except for HDL-cholesterol where it was elevated significantly in acute period. Carbon tetrachloride treatment increased the hepatic MDA and NO concentration in both acute and chronic groups. Rosemary extract did not alter MDA and NO concentrations induced by CCl4.As a conclusion, 200mg/kg RE have no effect against liver damage induced by CClin acute and chronic periods. It concluded that further studies on the different durations and different doses without any side effect of RE are in need to point out their possible effects.

Anahtar Kelime:

Konular: Tıbbi Araştırmalar Deneysel Genel ve Dahili Tıp Pediatri

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	YAMAN D, ATASEVER A (2016). Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi. , 83 - 100.
Chicago	YAMAN Duygu,ATASEVER Ayhan Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi. (2016): 83 - 100.
MLA	YAMAN Duygu,ATASEVER Ayhan Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi. , 2016, ss.83 - 100.
AMA	YAMAN D,ATASEVER A Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi. . 2016; 83 - 100.
Vancouver	YAMAN D,ATASEVER A Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi. . 2016; 83 - 100.
IEEE	YAMAN D,ATASEVER A "Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi." , ss.83 - 100, 2016.
ISNAD	YAMAN, Duygu - ATASEVER, Ayhan. "Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi". (2016), 83-100.

APA	YAMAN D, ATASEVER A (2016). Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 13(2), 83 - 100.
Chicago	YAMAN Duygu,ATASEVER Ayhan Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 13, no.2 (2016): 83 - 100.
MLA	YAMAN Duygu,ATASEVER Ayhan Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, vol.13, no.2, 2016, ss.83 - 100.
AMA	YAMAN D,ATASEVER A Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 2016; 13(2): 83 - 100.
Vancouver	YAMAN D,ATASEVER A Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 2016; 13(2): 83 - 100.
IEEE	YAMAN D,ATASEVER A "Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi." Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 13, ss.83 - 100, 2016.
ISNAD	YAMAN, Duygu - ATASEVER, Ayhan. "Karbon Tetraklorür ile Oluşturulan Akut ve Kronik Karaciğer Hasarı Üzerine Biberiye Ekstraktının (Rosmarinus officinalis) Etkisi". Erciyes Üniversitesi Veteriner Fakültesi Dergisi 13/2 (2016), 83-100.