The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study

SANCAKDAR, Enver; Tuzcu, Mehmet; Atabey, Mustafa; TOPÇU, Ömer; DUGER, CEVDET; DEVECİ, Köksal; GÖKAKIN, Ali Kağan

The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study

Ali Kağan GÖKAKIN, (Department of General Surgery, Cumhuriyet University Faculty of Medicine, Sivas;)

Mustafa ATABEY, (Department of General Surgery, Cumhuriyet University Faculty of Medicine, Sivas;)

Köksal DEVECİ, (Department of Biochemistry, Cumhuriyet University Faculty of Medicine, Sivas)

Enver SANCAKDAR, (Department of Biochemistry, Cumhuriyet University Faculty of Medicine, Sivas)

Mehmet TUZCU, (Department of Pathology, Cumhuriyet University Faculty of Veterinary, Sivas)

Cevdet DÜGER, (Department of Anesthesiology, Cumhuriyet University Faculty of Medicine, Sivas)

Ömer TOPÇU (Department of General Surgery, Cumhuriyet University Faculty of Medicine, Sivas;)

Ulusal Travma ve Acil Cerrahi Dergisi

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Yıl: 2014 Cilt: 20 Sayı: 5 Sayfa Aralığı: 319 - 327 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study

Öz:

AMAÇ: Ağır yanıklar sistemik enflamasyonu ve reaktif oksijen radikallerinin oluşumunu artırarak lipid peroksidasyonuna öncülük eder ve bu da uzak organ hasarında rol oynayabilir. Sildenafil selektif ve potent bir cyclic guanosine monofosfat ve spesifik bir fosfodiesteraz-5 inhibitörüdür. Sildenafil uzak organlarda enflamasyonu ve oksidatif stresi azaltır. Bu çalışmada, sildenafilin farklı dozlarda uzak organ hasarı üzerine olan etkisi araştırıldı. GEREÇ VE YÖNTEM: Otuz iki sıçan dört eşit gruba randomize şekilde ayrıldı. Sırasıyla; Sham, kontrol, 10 ve 20 mg/kg sildenafil tedavi grubu olarak adlandırıldı. Doku ve serumda malondialdehit (MDA), vasküler endotelyal büyüme faktörü (VEGF), VEGF reseptör (Flt-1), glutatyon peroksidaz aktivitesi (Gpx), total antioksidan kapasite (TAC) ve total oksidan durum (TOS) seviyeleri ölçüldü. Histopatolojik bulguların değerlendirilmesinde semi kantitatif skorlama sistemi kullanıldı. BULGULAR: Sildenafilin dokuda Gpx ve Flt değerlerini artırırken, MDA ve VEGF değerlerini azalttığı görüldü. Sildenafilin TAC ve Flt-1 serum seviyelerini artırdığı tespit edilse de TOS, OSI ve VEGF seviyelerini azalttığı görüldü. TARTIŞMA: Sildenafil histopatolojik incelemede uzak organ emflamasyon skorunu azaltır. Sildenafil ağır haşlama yanığına bağlı uzak organ hasarında oksidatif stres ve emflamasyonu azaltarak koruyucu etkiye sahiptir.

Anahtar Kelime:

Konular: Cerrahi

Sildenafilin ağır haşlama yanığı oluşturulan sıçan modelinde karaciğer ve böbrek hasarı üzerine etkisi: Biyokimyasal ve histopatolojik çalışma

Öz:

AMAÇ: Ağır yanıklar sistemik enflamasyonu ve reaktif oksijen radikallerinin oluşumunu artırarak lipid peroksidasyonuna öncülük eder ve bu da uzakorgan hasarında rol oynayabilir. Sildenafil selektif ve potent bir cyclic guanosine monofosfat ve spesifik bir fosfodiesteraz-5 inhibitörüdür. Sildenafiluzak organlarda enflamasyonu ve oksidatif stresi azaltır. Bu çalışmada, sildenafilin farklı dozlarda uzak organ hasarı üzerine olan etkisi araştırıldı.GEREÇ VE YÖNTEM: Otuz iki sıçan dört eşit gruba randomize şekilde ayrıldı. Sırasıyla; Sham, kontrol, 10 ve 20 mg/kg sildenafil tedavi grubu olarakadlandırıldı. Doku ve serumda malondialdehit (MDA), vasküler endotelyal büyüme faktörü (VEGF), VEGF reseptör (Flt-1), glutatyon peroksidazaktivitesi (Gpx), total antioksidan kapasite (TAC) ve total oksidan durum (TOS) seviyeleri ölçüldü. Histopatolojik bulguların değerlendirilmesindesemi kantitatif skorlama sistemi kullanıldı.BULGULAR: Sildenafilin dokuda Gpx ve Flt değerlerini artırırken, MDA ve VEGF değerlerini azalttığı görüldü. Sildenafilin TAC ve Flt-1 serum seviyeleriniartırdığı tespit edilse de TOS, OSI ve VEGF seviyelerini azalttığı görüldü.TARTIŞMA: Sildenafil histopatolojik incelemede uzak organ emflamasyon skorunu azaltır. Sildenafil ağır haşlama yanığına bağlı uzak organ hasarındaoksidatif stres ve emflamasyonu azaltarak koruyucu etkiye sahiptir.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	GÖKAKIN A, Atabey M, DEVECİ K, SANCAKDAR E, Tuzcu M, DUGER C, TOPÇU Ö (2014). The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study. , 319 - 327.
Chicago	GÖKAKIN Ali Kağan,Atabey Mustafa,DEVECİ Köksal,SANCAKDAR Enver,Tuzcu Mehmet,DUGER CEVDET,TOPÇU Ömer The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study. (2014): 319 - 327.
MLA	GÖKAKIN Ali Kağan,Atabey Mustafa,DEVECİ Köksal,SANCAKDAR Enver,Tuzcu Mehmet,DUGER CEVDET,TOPÇU Ömer The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study. , 2014, ss.319 - 327.
AMA	GÖKAKIN A,Atabey M,DEVECİ K,SANCAKDAR E,Tuzcu M,DUGER C,TOPÇU Ö The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study. . 2014; 319 - 327.
Vancouver	GÖKAKIN A,Atabey M,DEVECİ K,SANCAKDAR E,Tuzcu M,DUGER C,TOPÇU Ö The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study. . 2014; 319 - 327.
IEEE	GÖKAKIN A,Atabey M,DEVECİ K,SANCAKDAR E,Tuzcu M,DUGER C,TOPÇU Ö "The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study." , ss.319 - 327, 2014.
ISNAD	GÖKAKIN, Ali Kağan vd. "The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study". (2014), 319-327.

APA	GÖKAKIN A, Atabey M, DEVECİ K, SANCAKDAR E, Tuzcu M, DUGER C, TOPÇU Ö (2014). The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study. Ulusal Travma ve Acil Cerrahi Dergisi, 20(5), 319 - 327.
Chicago	GÖKAKIN Ali Kağan,Atabey Mustafa,DEVECİ Köksal,SANCAKDAR Enver,Tuzcu Mehmet,DUGER CEVDET,TOPÇU Ömer The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study. Ulusal Travma ve Acil Cerrahi Dergisi 20, no.5 (2014): 319 - 327.
MLA	GÖKAKIN Ali Kağan,Atabey Mustafa,DEVECİ Köksal,SANCAKDAR Enver,Tuzcu Mehmet,DUGER CEVDET,TOPÇU Ömer The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study. Ulusal Travma ve Acil Cerrahi Dergisi, vol.20, no.5, 2014, ss.319 - 327.
AMA	GÖKAKIN A,Atabey M,DEVECİ K,SANCAKDAR E,Tuzcu M,DUGER C,TOPÇU Ö The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study. Ulusal Travma ve Acil Cerrahi Dergisi. 2014; 20(5): 319 - 327.
Vancouver	GÖKAKIN A,Atabey M,DEVECİ K,SANCAKDAR E,Tuzcu M,DUGER C,TOPÇU Ö The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study. Ulusal Travma ve Acil Cerrahi Dergisi. 2014; 20(5): 319 - 327.
IEEE	GÖKAKIN A,Atabey M,DEVECİ K,SANCAKDAR E,Tuzcu M,DUGER C,TOPÇU Ö "The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study." Ulusal Travma ve Acil Cerrahi Dergisi, 20, ss.319 - 327, 2014.
ISNAD	GÖKAKIN, Ali Kağan vd. "The effects of sildenafil in liver and kidney injury in a rat model of severe scald burn: a biochemical and histopathological study". Ulusal Travma ve Acil Cerrahi Dergisi 20/5 (2014), 319-327.