The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk

Ijaz, Muhammad; AQIB, Amjad Islam; Hussain, Kashif; MALIK, Muhammad Abdul Rauf; FAROOQI, Shahid Hussain

The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk

Muhammad Abdul Rauf MALIK, (University of Veterinary and Animal Sciences, Outfall Road, 54000 Lahore - PAKISTAN)

Muhammad IJAZ, (University of Veterinary and Animal Sciences, Outfall Road, 54000 Lahore - PAKISTAN)

Amjad Islam AQIB, (University of Veterinary and Animal Sciences, Outfall Road, 54000 Lahore - PAKISTAN)

Shahid Hussain FAROOQI, (University of Veterinary and Animal Sciences, Outfall Road, 54000 Lahore - PAKISTAN)

Kashif HUSSAIN (University of Veterinary and Animal Sciences, Outfall Road, 54000 Lahore - PAKISTAN)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2017 Cilt: 23 Sayı: 5 Sayfa Aralığı: 809 - 815 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk

Öz:

Bu çalışma; zoonotik bir patojen olan koagülaz pozitif Staphylococcus aureus'un prevalansını ve bu patojenin yayılmasında rol oynayan risk fakörlerini tanımlamaktadır. Faisalabad'ın 3 ayrı bölgesindeki hayvanlardan toplanan toplam 900 adet süt örneğinde (n = 450 sığır ve n = 450 manda) Staphylococci identifikasyonu amacıyla mikrobiyolojik analiz ve coa geninin tespiti amacıyla PCR gerçekleştirildi. Subklinik mastitisin taranmasında sağım esnasında sörf mastitis testi uygulandı. Risk faktörlerinin mastitisle ilişkisini değerlendirmek amacıyla Ki-kare testi kullanıldı. Sığır ve manda sütlerinde subklinik mastitis prevalansı sırasıyla %54 ve %56'sı olarak hesaplanırken toplamda %55 olarak bulundu. Farklı şehirlerden örneklenen sığırlar arasında subklinik mastitis prevalansı yönünden anlamlı bir fark tespit edildi (P<0.05). Coa geninin prevalansı toplam örneklerde %39.33 olarak saptanmıştır. En yüksek prevalans oranı %43 olarak Samundari'de saptanırken bunu sırasıyla %39 ile Jaranwala ve %36 ile Faisalabad izlemektedir. Subklinik mastitisin meme loblarındaki prevalansı %32 olarak saptanırken meme loblarının %5.58'inde ise körelme belirlendi. Sağ taraftaki meme lobları ve meme başlarının daha fazla etkilendiği saptandı. Vücut kondisyonu ve doğum sayısı dışındaki risk faktörleri mastitis ile anlamlı ilişki içeriyordu. Çalışma, bakteriyel coa geninin sütte yaygınlığını ve göstergelerin yüksek prevalans ile kuvvetli bir ilişkisi olduğunu göstermektedir

Anahtar Kelime:

Konular: Veterinerlik

İnek Sütünde Koagulaz Pozitif Staphylococcus aureus Coa Geni Prevalansı ve İlgili Risk Faktörleri

Öz:

The study describes prevalence of coagulase positive Staphylococcus aureus, a zoonotic pathogen, and rule out key risk factors associated with the spread of this pathogen. A total of 900 milk samples of animals (n = 450 cattle and n = 450 buffaloes) from three subdistricts of Faisalabad were collected and processed microbiologically for staphylococci identification while, PCR for coa gene identification. Surf field mastitis test was used to screen the samples for subclinical mastitis at milking. Chi-square test was used to assess association of risk factors with mastitis. The overall prevalence of subclinical mastitis was 55% comprising 54 and 56% in cattle and buffalo’s milk, respectively. A significant difference (P<0.05) was seen in the prevalence of subclinical mastitis among the cattle from different cities. The prevalence of coa gene presented 39.33% from total samples. Highest prevalence was found in tehsil Samundari followed by Jaranwala and Faisalabad presenting 43%, 39%, and 36%, respectively. The quarter based prevalence of subclinical mastitis was found 32% while 5.58% of quarters were blind. Right side quarters and teats were more affected. The risk factors except body condition and parity presented significant association with mastitis. The study concluded that bacterial coa gene, was prevalent in milk, and determinants were strongly associated with higher prevalence

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	MALIK M, Ijaz M, AQIB A, FAROOQI S, Hussain K (2017). The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk. , 809 - 815.
Chicago	MALIK Muhammad Abdul Rauf,Ijaz Muhammad,AQIB Amjad Islam,FAROOQI Shahid Hussain,Hussain Kashif The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk. (2017): 809 - 815.
MLA	MALIK Muhammad Abdul Rauf,Ijaz Muhammad,AQIB Amjad Islam,FAROOQI Shahid Hussain,Hussain Kashif The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk. , 2017, ss.809 - 815.
AMA	MALIK M,Ijaz M,AQIB A,FAROOQI S,Hussain K The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk. . 2017; 809 - 815.
Vancouver	MALIK M,Ijaz M,AQIB A,FAROOQI S,Hussain K The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk. . 2017; 809 - 815.
IEEE	MALIK M,Ijaz M,AQIB A,FAROOQI S,Hussain K "The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk." , ss.809 - 815, 2017.
ISNAD	MALIK, Muhammad Abdul Rauf vd. "The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk". (2017), 809-815.

APA	MALIK M, Ijaz M, AQIB A, FAROOQI S, Hussain K (2017). The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(5), 809 - 815.
Chicago	MALIK Muhammad Abdul Rauf,Ijaz Muhammad,AQIB Amjad Islam,FAROOQI Shahid Hussain,Hussain Kashif The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 23, no.5 (2017): 809 - 815.
MLA	MALIK Muhammad Abdul Rauf,Ijaz Muhammad,AQIB Amjad Islam,FAROOQI Shahid Hussain,Hussain Kashif The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.23, no.5, 2017, ss.809 - 815.
AMA	MALIK M,Ijaz M,AQIB A,FAROOQI S,Hussain K The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2017; 23(5): 809 - 815.
Vancouver	MALIK M,Ijaz M,AQIB A,FAROOQI S,Hussain K The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2017; 23(5): 809 - 815.
IEEE	MALIK M,Ijaz M,AQIB A,FAROOQI S,Hussain K "The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23, ss.809 - 815, 2017.
ISNAD	MALIK, Muhammad Abdul Rauf vd. "The Prevalence and Associated Risk Factors of Coa Gene (Coagulase Positive Staphylococcus aureus) from Bovine Milk". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 23/5 (2017), 809-815.