Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique

Alkan, Feray

Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique

Feray ALKAN (Ankara Üniversitesi, Veteriner Fakültesi, Viroloji Anabilim Dalı, Ankara, Türkiye)

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 1998 Cilt: 45 Sayı: 1 Sayfa Aralığı: 181 - 184 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique

Öz:

Bu çalışmada, 8 farklı sürüde bulunan solunum sistemi enfeksiyonu bulgularına sahip 64 sığıra ait nasal swap materyalinden hazırlanan epitel hücre preparatlari direkt immunfloresan tekniği ile Bovine Adenovirus subgrup 1 antijenleri yönünden kontrol edildi. Örneklenen 8 sürüden 2 adedinde ve 64 sığırdan 3 adedinde Bovine adenovirus enfeksiyonu saptandı (BAVtesbiti bir sürüde 2/5, diğerinde 1/6). Elde edilen bulgular Bovine adenoviruslann sığırların solunum sistemi enfeksiyonlarında önemli bir etiyolojik ajan olabildiğini ve nasal swap örneklerinde antijen saptanması esasına dayanan direkt immunofloresan tekniğinin BAV'ların neden olduğu solunum sistemi enfeksiyonlarında etiyolojik ajanın saptanmasında kısa sürede uygulanan,duyarlı bir yöntem olduğunu ortaya koydu.

Anahtar Kelime: sığır burun solunum yolu hastalıkları teşhis etiyoloji hastalık tetkiki hastalık yaygınlığı immünofloresan antijenler Adenoviridae tanı teknikleri viral hastalıklar

Konular: Veterinerlik

Akut solunum sistemi enfeksiyonu bulguları gösteren sığırlarda bavine adenovirus subgrup 1 enfeksiyonlarının direkt immunofloresan testi ile çabuk teşhisi

Öz:

Nasal cells extracted from nasal swabs obtained from sixty four cattle with signs of respiratory disease ,from 8 different herds, were tested for Bovine adenoviruses subgroup 1 antigens using direct immunofluorescence technique. BAdV antigen positive samples were detected in two of eight herds examined. Of the 64 individual diseased cattle, three were found positive for BAdV subgroup 1 viral antigen ( 2/5 samples from one herd, 1/6 from the other). The findings reveal that BAdVs may be an important causative agent in cattle respiratory disease and direct immunofluorescence technique as a rapid method, based on the detection of antigen in nasal swab samples, has been used to establish the viral aetiology of acute respiratory disease caused BAdVs subgroup 1 .

Anahtar Kelime: disease surveys disease prevalence immunofluorescence antigens Adenoviridae diagnostic techniques viral diseases cattle nose respiratory diseases diagnosis aetiology

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

APA	Alkan F (1998). Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique. , 181 - 184.
Chicago	Alkan Feray Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique. (1998): 181 - 184.
MLA	Alkan Feray Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique. , 1998, ss.181 - 184.
AMA	Alkan F Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique. . 1998; 181 - 184.
Vancouver	Alkan F Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique. . 1998; 181 - 184.
IEEE	Alkan F "Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique." , ss.181 - 184, 1998.
ISNAD	Alkan, Feray. "Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique". (1998), 181-184.

APA	Alkan F (1998). Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 45(1), 181 - 184.
Chicago	Alkan Feray Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique. Ankara Üniversitesi Veteriner Fakültesi Dergisi 45, no.1 (1998): 181 - 184.
MLA	Alkan Feray Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.45, no.1, 1998, ss.181 - 184.
AMA	Alkan F Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 1998; 45(1): 181 - 184.
Vancouver	Alkan F Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 1998; 45(1): 181 - 184.
IEEE	Alkan F "Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 45, ss.181 - 184, 1998.
ISNAD	Alkan, Feray. "Rapid diagnosis of bovine adenovirus subgroup 1 infections in cattle with acute respiratory disease by direct immunofluorescence technique". Ankara Üniversitesi Veteriner Fakültesi Dergisi 45/1 (1998), 181-184.