The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress
Yıl: 2017 Cilt: 23 Sayı: 2 Sayfa Aralığı: 167 - 176 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress
Öz: Melatonin bir hayvansal hormon olarak ilk olarak sığır beyin üstü bezinden yarım yüzyılı aşkın bir süre önce izole edilmiş ve daha sonra tek hücreliler, mantarlar, algler, hayvanlar ve bitkiler gibi evrimsel olarak birbirlerinden çokfarklı organizmalarda varlığı kanıtlanmıştır. Bu çalışmada dışarıdan yapılan melatonin uygulamaları ile biberde (Capsicum annuum L.) çimlenme sırasında üşüme stresine karşı toleransın arttırılması hedeflenmiştir. Biber tohumları 24 saat süreyle farklı konsantrasyonlarda (0, 1, 5, 10 ve 25 µM) melatonin ile muamele edilmişler ve daha sonra bir gün kurutularak optimum (25 o C) ve üşüme stresi (15 o C) koşullarında çimlenme ve çıkış testlerine tabi tutulmuşlardır. Ekim öncesi tohum muamelesi şeklinde yapılan melatonin uygulamaları ile üşüme stresi koşulları altında biberin tohum çimlenmesi ve fide çıkış performansının olumlu yönde etkilenebileceği görülmüştür. En etkili melatonin konsantrasyonu olarak belirlenen 1 ve 5 µM melatonin uygulamaları sonucunda kontrol uygulamalarına kıyasla çimlenme ve çıkış yüzdeleri ile hızlarının arttığı saptanmıştır. Melatonin uygulamaları fidelerde H2 O2 ve MDA içeriğini düşürmüş, buna karşılık SOD ve CAT enzim aktivitelerini arttırmıştır. Bu araştırma sonuçlarına dayanarak, biber tohumlarının çimlenme ve fide çıkış performanslarının arttırılmasında antioksidan enzim aktivitelerinin seviyelerindeki artışın neden olduğu dokulardaki lipitlerin peroksidayonunda gerçekleşen bozulmanın azalması olduğu söylenebilir
Anahtar Kelime: Konular:
Melatonin Uygulamalarının Üşüme Stresi Altındaki Biber Tohumlarının Çimlenme ve Çıkış Performansı Üzerine Etkisi
Öz: Melatonin was first isolated from bovine pineal gland more than half a century ago as an important animal hormone and since then it was proved to be present in almost all forms of life including eukaryotic unicells, prokaryotes, fungi, algae, animals and plants. In this study, the effects of pre-sowing seed treatment with melatonin on germination and emergence performance of pepper seeds under chilling conditions were investigated. Seeds were immersed in 0 (distilled water), 1, 5, 10 or 25 µM melatonin solutions for 24 hours after which they were dried for one day and subjected to germination and emergence tests at optimum (25 oC) and chilling stress (15 oC) conditions. Untreated (dry) seeds were used as a control. Exogenous melatonin treatment promoted pepper seed germination and emergence under chilling conditions. Treatment of seeds with melatonin especially in 1 or 5 µM concentrations significantly improved germination and emergence percentage whereas control seeds and seeds treated with water exhibited the lowest germination and emergence performance. Melatonin application also reduced the MDA and H2O2 contents and elevated SOD and CAT enzyme activities. The improvement in germination and emergence performance of pepper under chilling stress conditions following melatonin treatment may therefore be due to reduced lipid peroxidation and elevated activities of antioxidant enzymes
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Afreen F, Zobayed S M & Kozai T (2006). Melatonin in Glycyrrhiza uralensis: Response of plant roots to spectral quality of light and UV-B radiation. Journal of Pineal Research 41: 108-115
- Arnao M B & Hernández-Ruiz J (2007). Melatonin promotes adventitious- and lateral root regeneration in etiolated hypocotyls of Lupinus albus L. Journal of Pineal Research 42: 147-152
- Bewley J D, Bradford K J, Hilhorst H W M & Nonogaki H (2013). Seeds: Physiology of Development, Germination and Dormancy, 3rd ed. Springer, New York
- Bowler C, Montagu M & Inze D (1992). Superoxide dismutase and stress tolerance. Annual Review of Plant Physiology and Plant Molecular Biology 43: 83-116
- Bradford M M (1976). A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of the protein-dye binding. Analytical Biochemistry 72: 248-254
- Chen G, Huo Y, Tan D X, Liang Z, Zhang W & Zhang Y (2003). Melatonin in Chinese medicinal herbs. Life Science 73: 19-26
- Çakmak I & Horst W J (1991). Effect of aluminum on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiologia Plantarum 83: 463-468
- Dey S K, Dey J, Patra S & Pothal D (2007). Changes in the antioxidative enzyme activities and lipid peroxidation in wheat seedlings exposed to cadmium and lead stress. Brazilian Journal of Plant Physiology 19: 53-60
- Dubbels R, Reiter R J, Klenke E, Goebel A, Schnakenberg E, Ehlers C, Schiwara H W & Schloot W (1995). Melatonin in edible plants identified by radioimmuno assay and by high performance liquid chromatographymass spectrometry. Journal of Pineal Research 18: 28-31
- Farooq M, Basra S M A, Ahmad N & Hafeez K (2005). Thermal Hardening: A new seed vigor enhancement tool in rice. Journal of Integrative Plant Biology 47: 187-193
- Gill S S & Tuteja N (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48: 909-930
- Hattori A, Migitata H, Iigo M, Itoh M, Yamamoto K, Ohtani-Kaneko R, Hara M, Suzuki T & Reiter R J (1995). Identification of melatonin in plants and its effects on plasma melatonin levels and binding to melatonin receptors in vertebrates. Biochemistry and Molecular Biology International 35: 627-634
- Herzog V & Fahimi H (1973). Determination of the activity of peroxidase. Analytical Biochemistry 55: 554-562
- Hodges D M, Andrews C J, Johnson D A & Hamilton R I (1997). Antioxidant enzyme responses to chilling stress in differentially sensitive inbred maize lines. Journal of Experimental Botany 48: 1105-1113
- ISTA (2007). International Rules for Seed Testing. International Seed Testing Association (ISTA) Basserdorf, Switzerland
- Kang H M & Saltveit M E (2002). Effect of chilling on antioxidant enzymes and DPPH-radical scavenging activity of high- and low-vigour cucumber seedling radicles. Plant, Cell and Environment 25: 1233-1238
- Karaca A (2013). Dışarıdan yapılan melatonin uygulamaları ile biberde çimlenme sırasında üşüme stresine karşı toleransın arttırılması. Yüksek lisans tezi, Kahramanmaraş Sütçü İmam Üniversitesi Fen Bilimleri Enstitüsü (Basılmamış), Kahramanmaraş
- Kolár J & Machácková I (1994). Melatonin: Does it regulate rhythmicity and photoperiodism also in higher plants? Flower Newsletter 17: 53-54
- Kolár J & Machácková I (2005). Melatonin in higher plants: occurrence and possible functions. Journal of Pineal Research 39: 333-341
- Kolár J, Johnson C H & Machácková I (1999). Presence and possible role of melatonin in a short-day flowering plant, Chenopodium rubrum. Advanced Experimental Medicinal Biology 460: 391-393
- Korkmaz A & Korkmaz Y (2009). Promotion by 5-aminolevulinic acid of pepper seed germination and seedling emergence under low temperature stress. Scientia Horticulturae 119: 98-102
- Korkmaz A, Değer Ö & Cuci Y (2014). Profiling the melatonin content in organs of the pepper plant during different growth stages. Scientia Horticulturae 172: 242-247
- Lerner A B, Case J D, Takahashi Y, Lee T H & Mori W (1958). Isolation of melatonin, the pineal factor that lightness melanocytes. Journal of American Chemistry Society 80: 2587-2592
- Li C, Wang P, Wei Z, Liang D, Liu C, Yin L, Jia D, Fu M & Ma F (2012). The mitigation effects of exogenous melatonin on salinity-induced stress in Malus hupehensis. Journal of Pineal Research 53: 298-306
- Lorenz O A & Maynard D R (1988). Knott’s handbook for vegetable growers, 3rd ed. Wiley, New York
- Okazaki M, Higuchi K, Aouini A & Ezura H (2010). Lowering intercellular melatonin levels by transgenic analysis of indoleamine 2, 3-dioxygenase from rice in tomato plants. Journal of Pineal Research 49: 239-247
- Özden M, Demirel U & Kahraman A (2009). Effects of proline on antioxidant system in leaves of grapevine (Vitis vinifera L.) exposed to oxidative stress by H2 O2.Scientia Horticulturae 119: 163-168
- Paredes S D, Korkmaz A, Manchester L C, Tan D X & Reiter R J (2009). Phytomelatonin: A review. Journal of Experimental Botany 60: 57-69
- Posmyk M M & Janas K M (2009). Melatonin in plants. Acta Physiologia Plantarum 31: 1-11
- Posmyk M M, Balabusta M, Wieczorek M, Sliwinska E & Janas K M (2009). Melatonin applied to cucumber (Cucumis sativus L.) seeds improves germination during chilling stress. Journal of Pineal Research 46: 214-223
- Reiter R, Tan D, Manchester L, Simopoulos A P, Maldonado M, Flores L & Terron M (2007a). Melatonin in edible plants (phytomelatonin): identification, concentrations, bioavailability and proposed functions. World Review of Nutrition and Dietetics 97: 211-230
- Reiter R J, Tan D X, Terron M P, Flores L J & Czarnocki Z (2007b). Melatonin and its metabolites: New findings regarding their production and their radical scavenging actions. Acta Biochimica Polonica 54: 1-9
- Sachs M, Cantliffe D J & Watkins J T (1980). Germination of pepper seed at low temperatures after various pretreatments. Proceedings of the Florida State Horticultural Society 93: 258-260
- Scandalios J G, Guan L & Polidoros A N (1997). Catalases in plants: Gene structure, properties, regulation and expression, In: J G Scandalios (Ed), Oxidative Stress and the Molecular Biology of Antioxidant Defenses. Cold Spring Harbor Laboratory Press, pp. 343-406, NY
- Seckin B, Turkan I, Sekmen A H & Ozfidan C (2010). The role of antioxidant defense systems at differential salt tolerance of Hordeum marinum Huds. (sea barleygrass) and Hordeum vulgare L. (cultivated barley). Environmental and Experimental Botany 69: 76-85
- Tan D X, Chen L D, Poeggeler B, Manchester L C & Reiter R J (1993). Melatonin: A potent, endogenous hydroxyl radical scavenger. Endocrinology Journal 1: 57-60
- Tan D X, Manchester L C, Reiter R J, Plummer B F, Limson J, Weintraub S T & Qi W (2000). Melatonin directly scavenges hydrogen peroxide: A potentially new metabolic pathway of melatonin bio-transformation. Free Radical Biology and Medicine 29: 1177-1185
- Tan D X, Hardeland R, Manchester L C, Korkmaz A, Ma S, Rosales-Corral S & Reiter R J (2012). Functional roles of melatonin in plants, and perspectives in nutritional and agricultural science. Journal of Experimental Botany 63: 577-597
- Tiryaki I & Keles H (2012). Reversal of the inhibitory effect of light and high temperature on germination of Phacelia tanacetifolia seeds by melatonin. Journal of Pineal Research 52: 332-339
- Van Tassel D L, Li J & O’Neill S D (1993). Melatonin: Identification of a potential dark signal in plants. Plant Physiology 102(Supplement 1): 659
- Wang P, Sun X, Li C, Wei Z, Liang D & Ma F (2013). Long-term exogenous application of melatonin delays drought-induced leaf senescence in apple. Journal of Pineal Research 54: 292-302
- Watkins J T & Cantliffe D J (1983). Mechanical resistance of the seed coat and endosperm during germination of Capsicum annuum L. at low temperature. Plant Physiology 72: 146-150
- Xu P L, Guo Y K, Bai J G, Shang L & Wang X J (2008). Effects of long-term chilling on ultrastructure and antioxidant activity in leaves of two cucumber cultivars under low light. Physiologia Plantarum 132: 467-478
- Zhang J H, Huang W D, Liu Y P & Pan Q H (2005). Effects of temperature acclimation pretreatment on the ultrastructure of mesophyll cells in young grape plants (Vitis vinifera L. cv. Jingxiu) under crosstemperature stresses. Journal of International Plant Biology 47: 959-970
- Zhang N, Zhao B, Zhang H J, Weeda S, Yang C, Yang Z C, Ren S & Guo Y D (2013). Melatonin promotes water-stress tolerance, lateral root formation, and seed germination in cucumber (Cucumis sativus L.). Journal of Pineal Research 54: 15-23
| APA | Korkmaz A, KARACA A, KOCAÇİNAR F, CUCI Y (2017). The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress. , 167 - 176. |
| Chicago | Korkmaz Ahmet,KARACA Aygül,KOCAÇİNAR Ferit,CUCI YAKUP The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress. (2017): 167 - 176. |
| MLA | Korkmaz Ahmet,KARACA Aygül,KOCAÇİNAR Ferit,CUCI YAKUP The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress. , 2017, ss.167 - 176. |
| AMA | Korkmaz A,KARACA A,KOCAÇİNAR F,CUCI Y The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress. . 2017; 167 - 176. |
| Vancouver | Korkmaz A,KARACA A,KOCAÇİNAR F,CUCI Y The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress. . 2017; 167 - 176. |
| IEEE | Korkmaz A,KARACA A,KOCAÇİNAR F,CUCI Y "The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress." , ss.167 - 176, 2017. |
| ISNAD | Korkmaz, Ahmet vd. "The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress". (2017), 167-176. |
| APA | Korkmaz A, KARACA A, KOCAÇİNAR F, CUCI Y (2017). The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress. Tarım Bilimleri Dergisi, 23(2), 167 - 176. |
| Chicago | Korkmaz Ahmet,KARACA Aygül,KOCAÇİNAR Ferit,CUCI YAKUP The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress. Tarım Bilimleri Dergisi 23, no.2 (2017): 167 - 176. |
| MLA | Korkmaz Ahmet,KARACA Aygül,KOCAÇİNAR Ferit,CUCI YAKUP The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress. Tarım Bilimleri Dergisi, vol.23, no.2, 2017, ss.167 - 176. |
| AMA | Korkmaz A,KARACA A,KOCAÇİNAR F,CUCI Y The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress. Tarım Bilimleri Dergisi. 2017; 23(2): 167 - 176. |
| Vancouver | Korkmaz A,KARACA A,KOCAÇİNAR F,CUCI Y The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress. Tarım Bilimleri Dergisi. 2017; 23(2): 167 - 176. |
| IEEE | Korkmaz A,KARACA A,KOCAÇİNAR F,CUCI Y "The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress." Tarım Bilimleri Dergisi, 23, ss.167 - 176, 2017. |
| ISNAD | Korkmaz, Ahmet vd. "The Effects of Seed Treatment with Melatonin on Germination and Emergence Performance of Pepper Seeds under Chilling Stress". Tarım Bilimleri Dergisi 23/2 (2017), 167-176. |
