Yıl: 2017 Cilt: 7 Sayı: 2 Sayfa Aralığı: 153 - 167 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi

Öz:
Meyve ve sebzelerde bulunan biyoaktif bileşenlere karşı ilgi, bu maddelerin insanları bazı hastalıklara karşı korumaya yardımcı olabilmesinden dolayı giderek artmaktadır. Bu kimyasalların, oksidatif stres sonucunda hücrelerde meydana gelen serbest radikal zararını azaltabildiği ve kanser, kardiyovasküler hastalık, obezite ve diğer hastalıklar gibi büyük kronik tabloların risklerini azaltması ile bağlantılı olduğu belirtilmektedir. Bu çalışmada turunçgil kabuklarının biyoaktif bileşenleri ve antioksidan özellikleri incelendi. Turunçgil kabukları limon (Citrus limon), portakal (Citrus sinensis), mandalina (Citrus reticulata) ve greyfurt (Citrus paradisi) meyvelerinden elde edildi. Toplam karotenoid, beta karoten, askorbik asit, antosiyanin, toplam fenolik ve flavonoid içerikleri ve toplam antioksidan aktiviteleri TEAC, FRAP ve DPPH metotlarına göre kolorimetrik yöntemler kullanılarak belirlendi. Mandalina kabuklarının karotenoid bileşikler, limon kabuklarının askorbik asit, greyfurt ve limon kabuklarının fenolik bileşikler açısından zengin olduğu ve mandalina ile portakal kabuklarına göre daha yüksek antioksidan aktiviteye sahip olduğu bulundu.
Anahtar Kelime:

Determination of Bioactive Compounds and Antioxidant Activities of Citrus Peels

Öz:
Bioactive compounds present in fruits and vegetables are receiving increased because they may help the protect humans against some diseases. These chemicals can reduce the free radicals damage caused to the cells, as a result of oxidative stress and they have been linked to reductions in the risk of major chronic diseases such as cancer, cardiovascular disease, obesity and other disease. In the present study, the bioactive compounds and antioxidant properties of citrus peels were examined. Fruit peels were obtained from lemon (Citrus limon), orange (Citrus sinensis, mandarin (Citrus reticulata) and grapefruit (Citrus paradisi). Total carotenoid, beta carotene, ascorbic acid, anthocyanin, total phenolic and flavonoid content were determined using colorimetric methods. The total antioxidant activities were analysed according to TEAC, FRAP and DPPH methods. It was found that mandarin and lemon peels were rich in carotenoid compounds and ascorbic acid, respectively while grapefruit and lemon peels were rich in phenolic compounds and had higher antioxidant activities than mandarin and orange peels.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • AACC, 2004. Aapproved Methods of the American Association of Cereal Chemists, 11th Edition.
  • Abozed, S.S., El-kalyoubi, M., Abdelrashid, A., Salama, M.F., 2014. Total phenolic contents and antioxidant activities of various solvent extracts from whole wheat and bran. Annals of Agric Sci., 59(1):63-67.
  • Acquistucci, R., Bucci, R., Magri, A.D., Magri, A.R., 1991. Evaluation of the moisture and ash contents in wheat mills by multistep programmed thermogravimetry. Thermochimica Acta, 188(1): 51-62.
  • Ali, J., Abid, H., Hussain, A., 2010. Study of some macronutrients composition in peels of different citrus fruits grown in NWFP. Journal of the Chemical Society of Pakistan, 32(1):83-86.
  • Al-Saadi, N.H.M., Ahmad, N.S., Saeed, S.E., 2009. Determination of some chemical compounds and the effect of oil extract from orange peel on some pathogens. Journal of Kerbala University, 7(2): 33-39.
  • AOAC, 1989. Officials Methods of Analysis, 72(3): 481-483.
  • Alkaya, E., Ergüder T.H. ve Demirer, G.N., 2010. Effect of operational parameters on anaerobic co-digestion of dairy cattle manure and agricultural residues: A case study for the Kahramanmaraş region in Turkey. Engineering in Life Sciences, 10(6):552-559.
  • Al-Saadi, N.H.M., Ahmad, N.S., Saeed, S.E., 2009. Determination of some chemical compounds and the effect of oil extract from orange peel on some pathogens. Journal of Kerbala University, 7(2):33-39.
  • Anonim, 2011. Aile ve tüketici hizmetleri, M.E.B. Çevre hizmetleri Modülü, Ankara. http://megep.meb.gov.tr/mte_program _modul/moduller_pdf/%C3%87evre% 20Hizmetleri.pdf (25.07.2016).
  • Anonim, 2014b. Fruit peel nutrition facts. http://www.nutrition-andyou.com/fruit-peel.html (19.10.2014).
  • Anonim, 2015. Bitkisel Üretim İstatistikleri. http://www.tuik.gov.tr/PreHaberBulte nleri.do?id=18706. Erişim Tarihi: 16.Ağustos 2016.
  • Anonim, 2016. Bitkisel Üretim İstatistikleri, 2015. http://www.tuik.gov.tr/PreHaberBulte nleri.do?id=18706 (16.08.2016).
  • Ashoush, I.S., Gadallah, M.G.E., 2011. Utilization of Mango Peels and Seed Kernels Powders as Sources of Phytochemicals in Biscuit. World Journal of Dairy & Food Sciences, 6 (1): 35-42.
  • Belitz, H.D., Grosch, W., 1999. Fruits and fruit products. In: Hadziyev D (ed) Food chemistry. Springern Verlag, Berlin, Heidelberg, pp 748-799.
  • Benzie, I. F. F. Strain, J. J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Analytical Biochemistry, 239: 70-76.
  • Brand-Williams, W., Cuvelier, M., Berset, C., 1995. Use of free radical method to evaluate antioxidant activity. Food Science and Technology, 28: 25-30.
  • Bocco, A., Cuvelier, M.E., Richard, H., Berset, C., 1998. Antioxidant Activity and Phenolic Composition of Citrus Peel and Seed Extracts. Agric. Food Chem. 46: 2123-2129.
  • Canan, İ., Gündoğdu, M., Seday, U., Oluk, C.A., Karaşahin, Z., Eroğlu, Ç.E., Yazıcı, E., Ünlü, M., 2016.
  • Determination of antioxidant, total phenolic, total carotenoid, lycopene, ascorbic acid and sugar contents of Citrus species and mandarin hybrids. Turkish Journal of Agriculture and Forestry, 40: 894-899.
  • Chang, C. C., Yang M.H., Wen, H.M., Chern, J.C., 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J. Food Drug Anal., 10: 178-182.
  • Chaovanalikit, A., Wrolstad, R. E., 2004. Total Anthocyanins and Total Phenolics of Fresh and Processed Cherries and Their Antioxidant Properties. Journal of Food Science, 69(1)-67-72.
  • Cheigh, C.I., Chung, E.Y., Chung, M.S., 2012. Enhanced extraction of flavanones hesperidin and narirutin from Citrus unshiu peel using subcritical water. J. Food Eng., 110: 472-477.
  • Damar, İ., 2010. Vişne Suyunun Antosiyanin Profili ve Antioksidan Kapasitesi. Yüksek Lisans Tezi, Ankara Üniversitesi.
  • Dent, M., Dragovi, D., Peni, M., Brncic, M., Bosiljkov, T., Levaj, B., 2013. The Effect of Extraction Solvents, Temperature and Time on the Composition and Mass Fraction of Polyphenols in Dalmatian Wild Sage (Salvia officinalis L.) Extracts. Food Technol. Biotechnol., 51(1) 84-91.
  • Diankov, S., Karsheva, M., Hinkov, I., 2011. Extraction of natural antioxidants from lemon peels. Kinetics and antioxidant capacity. Journal of the University of Chemical Technology and Metallurgy, 46(3): 315-319.
  • Fathordoobady, F., Mirhosseini, H., Selamat, J., Manap, M.Y.A., 2016. Effect of solvent type and ratio on betacyanins and antioxidant activity of extracts from Hylocereus polyrhizus flesh and peel by supercritical fluid extraction and solvent extraction. Food Chemistry, 202:70-80.
  • Fidrianny, I., Harnovi, M., Insanu, M., 2014. Evaluation Of Antioxidant Activities From Various Extracts Of Sweet Orange Peels Using Dpph, Frap Assays And Correlation With Phenolic, Flavonoid, Carotenoid Content. Asian J Pharm Clin Res, 7(3):186-190.
  • Friedman, M., Levin, C., 2009. Analysis and biological activities of potato glycoalkaloids, calystegine alkaloids, phenolic compounds and anthocyanins. In J. Singh, & L. Kaur (Eds.) Advances in Potato Chemistry and Technology, pp. 127-161.
  • Fuleki, T., Francis, F., 1968. Quantitative methods for anthocyanins. J. Food Science, 33: 72-77.
  • Garcia, E.M., Barret, D., 2005. Assessing lycopene content in California processing tomatoes. Journal of Food Processing and Preservation, 30: 56- 70.
  • Gao, L., Girard, B., Mazza, G., Reynolds, A.G., 1997. Changes in Anthocyanins and Color Characteristics of Pinot Noir Wines during Different Vinification Process. J. Agric. Food Chem., 45: 2003-2008.
  • Garmusa, T.T., Paviania, L.C., Queirogab, C.L. , Magalhãesb, P.M., Cabral, F.A., 2014. Extraction of phenolic compounds from pitanga (Eugenia uniflora L.)leaves by sequential extraction in fixed bed extractor usingsupercritical CO2, ethanol and water as solvents. J. of Supercritical Fluids 86: 4-14.
  • Ghasemi, K., Ghasemi, Y., Ebrahimzadeh, M.A., 2009. Antioxidant Activity, Phenol And Flavonoid Contents of 13 Citrus Species Peels And Tissues. Pak. J. Pharm. Sci., 22(3): 277-281.
  • Gorinstein, S., Zachwieja, Z., Katrich, E., Pawelzik, E., Haruenkit, R., Trakhtenberg, S. ve Martin-Belloso, O., 2004. Comparison of the contents of the main antioxidant compounds and the antioxidant activity of white grapefruit and his new hybrid. LWT - Food Sci. Tech., 37: 337-343.
  • Goulas, V. ve Manganaris, G.A., 2012. Exploring the phytochemical content and the antioxidant potential of Citrus fruits grown in Cyprus. Food Chem., 131:39-47.
  • Guardia, T., Rotelli, A.E., Juarez, A.O., Pelzer, L.E., 2001. Antiinflamatory properties of plant flavonoids.Effects of rutin, quarcetin and hesperidin on adjuvant arthritis in rat, Il Farmaco, 56(9):683-687.
  • Hışıl, 2004. Enstrümental gıda analizlerilaboratuar deneyleri. Ege Üniversitesi Mühendislik Fakültesi Ders Kitapları Yayın no:45, Bornova, İzmir.
  • Hijazi, A., Al Masri, D.S., Farhan, H., Nasser, M., Rammal, H., Annan, H., 2015. J Pharm Chem Biol Sci ,3(2):262-271.
  • Horowitz, R.M., 1961. The citrus flavonoids, The Orange;Its Biochemistry and Physiology, Sinclair, W.B.(Ed.)
  • Janati, S. S. F., Beheshti, H. R., Feizy, J., Fahim, N. K., 2012. Chemical Composition Of Lemon (Citrus Limon) And Peels Its Considerations As Animal Food. Gıda 37 (5): 267- 271.
  • Kumar, P.S., Suresh. E., Kalavathy, K., 2013. Review on a potential herb Calotropis gigantea (L.) R. Br., Sch. Acad. J. Pharm., 2(2):135-143.
  • Larrauri, J. A., Ruperez, P., ve Saura-calixto, F.,1999. New approaches in the preparation of high dietary fibre from fruit by-products. Trends in Food Science and Technology, 29: 729-733.
  • Lee, Y.H., Charles, A.L., Kung, H.F., Ho, H.F., Huang, T.C., 2010. Extraction of nobiletin and tangeretin from Citrus depressa Hayata by supercritical carbon dioxide with ethanol as modifier. Ind. Crops Prod., 31: 59-64.
  • Li, B. B., Smith, B., Hossain, M., 2006. Extraction of phenolics from citrus peels. I. Solvent extraction method. Sep. Purif. Technol., 48: 182-188.
  • Li, Y., Ma, D., Sun, D., Wang, C., Zhang, J., Xie, Y., Guo, T., 2015. Total phenolic, flavonoid content, and antioxidant activity of flour, noodles, and steamed bread made from different colored wheat grains by three milling methods. The crop journal 3: 328-334.
  • Lim, Y.Y., Lim T.T. ve Tee J.J., 2006. Antioxidant properties of guava fruit: comparison with some local fruits. Sunway Acad. J., 3: 9-20.
  • Londono, J.L., Lima, V.R., Lara, O., Gil, A., Pasa, T.B.C., 2010. Clean recovery of antioxidant flavonoids from citrus peel: Optimizing an aqueous ultrasound-assisted extraction method. Food Chem., 119: 81-87. Luterotti, S., Kljak, K., 2010.
  • Spectrophotometric Estimation of Total Carotenoids in Cereal Grain Products. Acta Chim. Slov. 57: 781- 787.
  • Manthey, J.A., Grohmann, K., 1996. Consantrations of hesperidin and other orange peel flavonoids in citrus processing byproducts, J. Agric. Food Chem., 44:811-814.
  • Mohammed, A.M.A., Ibrahim, A.M.,, Omran, A.A., Mohamed, M.E., Elsheikh, S.E.M., 2013. Minerals content, essential oils composition and physicochemical properties of Citrus jambhiri Lush. (Rough Lemon) from the Sudan. International Letters of Chemistry, Physics and Astronomy, 9(1): 25-30.
  • Moon, J.K., Shibamoto T., 2009. Antioxidant assays for plant and food components. J. Agric. Food Chem., 57:1655-1666. Oboh, G., Ademosun, A.O., 2012.
  • Characterization of the antioxidant properties of phenolic extracts from some citrus peels. J Food Sci Technol 49(6): 729-736.
  • Okan, O.T., Varlıbaş, H., Öz, M., Deniz, İ., 2013. Antioksidan Analiz Yöntemleri ve Doğu Karadeniz Bölgesinde Antioksidan Kaynağı Olarak Kullanılabilecek Odun Dışı Bazı Bitkisel Ürünler. Kastamonu Üni., Orman Fakültesi Dergisi, 13(1): 48- 59.
  • Osarumwense, P.O., Okunrobo, L.O., Uwumarongie-ilori, E.G., 2013.
  • Phytochemical screening, proximate and elemental analysis of Citrus sinensis peels (l.) Osbeck, J. Appl. Sci. Environ. Manage., 17: 47-50.
  • Özyurt, D., 2005. Toplam Flavonoid Miktarının Geliştirilen Spektrofotometrik Yöntem ile Tayini. Yüksek Lisans Tezi, İstanbul Teknik Üniversitesi.
  • Paula, J.T., Paviani, L.C., Foglio, M.A., Sousa, I.M.O., Duarte, G.H.B., Jorge, M.P., Eberlin, M.N., Cabral, F.A.,
  • 2014. Extraction of anthocyanins and luteolin from Arrabidaea chica by sequential extraction in fixed bed using supercritical CO2, ethanol and water as solvents. The Journal of Supercritical Fluids, 86:100-107.
  • Prior, R.L., Wu, X., Schaich, K., 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J. Agric. Food Chem., 53: 4290-4303.
  • Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M. ve Rice-Evans, C., 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26: 1231-1237.
  • Samuagam, L., Sia, C.M., Akowuah, G.A., Okechukwu, P.N., Yim, H.S., 2013.
  • The effect of extraction conditions on total phenolic content and free radical scavenging capacity of selected tropical fruits'peel. Health and the Environment Journal, 4(2):80-102.
  • Sanusi, R. A. ve Adebiyi, A. E., 2009. Beta Carotene Content of Commonly Consumed Foods and Soups in Nigeria. Pakistan Journal of Nutrition, 8(9): 1512-1516.
  • Singh, S., Immanuel, G., 2014. Extraction of antioxidants from fruit peels and its utilization in paneer. J Food Process Technol., 5:349.
  • Singleton, V.L., Rossi, J.A., 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticuture, 16: 144-158.
  • Song, F., Parekh, S., Hooper, L., Loke, Y.K., Ryder, J.J., Sutton, A.J., Hing, C.,
  • Kwok, C.S., Pang, C., Harvey, I.,2010. Dissemination and publication of research findings: an updated review of related biases. Health Technology Assessment, 14:8.
  • Souza, V.R., Pereira, P.A.P., Silva, T.L.T., Lima, L.C.O., Pio, R., Queiroz F.,
  • 2014. Determination of the bioactive compounds, antioxidant activity and chemical composition of Brazilian blackberry, red raspberry, strawberry, blueberry and sweet cherry fruits. Food Chemistry 156: 362-368.
  • Stahl, W., Sies, H., 1996. Perspective in biochemistry and biophysics. Archive of Biochemistry and Bıophysics. 336: 1-9.
  • Tumbas, V.T., Cetkovic, G.S., Djilas, S.M., Canadanovic-Brunet, J.M., Vulic, J.J., Knez, Z., 2010. Antioxidant activity of mandarin (Citrus reticulata) peel. Biblid., 40: 195-203.
  • Turhan, S., Üstün, N.Ş., 2006. Doğal antioksidanlar ve gıdalarda kullanımı. Türkiye 9. Gıda Kongresi, 24-26 Mayıs, 2006, Bolu.
  • Turhan, İ., Tetik, N., Karhan, M., 2006. Turunçgil kabuk yağlarının elde edilmesi ve gıda endüstrisinde kullanımı. Gıda Teknolojileri Elektronik Dergisi, 3:71-77.
  • Vieira, F. G. K., Borges, G. S. C., Copetti, C., Gonzaga, L. V., Nunes, E. C., Fett, R., 2009. Activity and contents of polyphenolic antioxidants in the whole fruit, flesh and peel of three apple cultivars. Archivos Latinoamericanos De Nutricion, 59(1):101-106.
  • Wang, Y.C., Chuang, Y.C., Hsu, H.W., 2008. The flavonoid, carotenoid and pectin content in peels of citrus cultivated in Taiwan. Food Chem., 106: 277-284.
  • Waszkowiak, K., Swig?o, A.G., 2016. Binary ethanol-water solvents affect phenolic profile and antioxidant capacity of flaxseed extracts. Eur Food Res Technol., 242:777-786.
  • Wilkins, M.R., Widmer, W.W., Grohmann, K., 2007. Simultaneous saccharification and fermentation of citrus peel waste by Saccharomyces cerevisiae to produce ethanol. Process Biochemistry 42: 1614-1619.
  • Wolfe, K., Wu, X., Liu, R.H., 2003. Antioxidant Activity of Apple Peels. J. Agric. Food Chem., 51: 609-614.
  • Wong Y.S., Sia C.H., Khoo H.E., Ang Y.K., Chang S.K., Yim H.S., 2014.
  • Influence of extraction conditions on antioxidant properties of passion fruit (Passiflora edulis) peel. Acta Sci. Pol., Technol. Aliment, 13(3): 257-265.
  • Yapo, B. M., 2009. Biochemical Characteristics and Gelling Capacity of Pectin from Yellow Passion Fruit Rind as Affected by Acid Extractant Nature. J. Agric. Food Chem., 57: 1572-1578.
APA GÜZEL M, AKPINAR O (2017). Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi. , 153 - 167.
Chicago GÜZEL MELIH,AKPINAR OZLEM Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi. (2017): 153 - 167.
MLA GÜZEL MELIH,AKPINAR OZLEM Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi. , 2017, ss.153 - 167.
AMA GÜZEL M,AKPINAR O Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi. . 2017; 153 - 167.
Vancouver GÜZEL M,AKPINAR O Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi. . 2017; 153 - 167.
IEEE GÜZEL M,AKPINAR O "Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi." , ss.153 - 167, 2017.
ISNAD GÜZEL, MELIH - AKPINAR, OZLEM. "Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi". (2017), 153-167.
APA GÜZEL M, AKPINAR O (2017). Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 7(2), 153 - 167.
Chicago GÜZEL MELIH,AKPINAR OZLEM Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi. Gümüşhane Üniversitesi Fen Bilimleri Dergisi 7, no.2 (2017): 153 - 167.
MLA GÜZEL MELIH,AKPINAR OZLEM Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, vol.7, no.2, 2017, ss.153 - 167.
AMA GÜZEL M,AKPINAR O Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi. Gümüşhane Üniversitesi Fen Bilimleri Dergisi. 2017; 7(2): 153 - 167.
Vancouver GÜZEL M,AKPINAR O Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi. Gümüşhane Üniversitesi Fen Bilimleri Dergisi. 2017; 7(2): 153 - 167.
IEEE GÜZEL M,AKPINAR O "Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi." Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 7, ss.153 - 167, 2017.
ISNAD GÜZEL, MELIH - AKPINAR, OZLEM. "Turunçgil Kabuklarının Biyoaktif Bileşenleri ve Antioksidan Aktivitelerinin Belirlenmesi". Gümüşhane Üniversitesi Fen Bilimleri Dergisi 7/2 (2017), 153-167.