Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi

DAĞ, Serpil; ÖZEN, Hasan; Çığşar, Gülşen; KARAMAN, Musa; ATAKiŞİ, Onur; Kaya, Oktay

Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi

Musa KARAMAN, (epartment of Pathology, Faculty of Veterinary Medicine, Balikesir University, TR-10145 Balikesir - TURKEY)

Hasan ÖZEN, (Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEY)

Serpil DAĞ, (Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEY)

Onur ATAKİŞİ, (Department of Pathology, Faculty of Veterinary Medicine, Kafkas University, TR-36100 Kars - TURKEY)

Gülşen ÇIĞŞAR, (Department of Emergency, Faculty of Medicine, Kafkas University, TR-36100 Kars - TURKEY)

Oktay KAYA (Department of Physiology, Faculty of Medicine, Trakya University, TR-22030 Edirne - TURKEY)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2017 Cilt: 23 Sayı: 1 Sayfa Aralığı: 77 - 85 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi

Öz:

Omega-3 bağışıklığı düzenleyici fonksiyonları olduğu bilinen çoklu doymamış bir yağ asididir. Bu çalışmada, deneysel karbon tetraklorür (CCl4) toksikasyonunda omega-3'ün koruyucu potansiyel etkisi araştırılmıştır. Toplam 40 adet ergin erkek Wistar albino rat eşit beş gruba ayrıldı ve 6 hafta süresince her iki günde bir olmak üzere subkutan yolla deneklere tarif edilen uygulamalar yapıldı; Grup 1 (kontrol): 0.5 ml/kg serum fizyolojik, Grup 2 (Omega): 0.5 g/kg omega-3, Grup 3 (Taşıt): 0.5 ml/kg saf zeytin yağı, Grup 4 (CCl4): 0.5 ml/kg CCl4, Grup 5 (CCl4 + Omega): 0.5 ml/kg CCl4 artı 0.5 g/kg omega-3. Araştırma süresinin sonunda, kan örnekleri alınan deneklere karaciğer doku örneklerinin toplanması amacıyla nekropsi uygulandı. Serum AST, AlT, GGT, TAC, TOC, trigliserid ve visfatin seviyeleri belirlendi. Karaciğer morfolojisi incelendi ve TGF-?, TGF-?, PPAR-? ve PPAR-? immunreaktiviteleri tespit edildi. Kontrol grubu ile karşılaştırıldığında CCl4 verilen hayvanlarda serum AST, AlT, GGT ve TOC seviyelerinin anlamlı derecede arttığı, TAC seviyesinin ise azaldığı gözlemlendi. Trigliserid ve visfatin seviyelerinde anlamlı bir değişikliğin olmadığı belirlendi. İmmunohistokimyasal boyamalarda CCl4 verilen hayvanlarda TGF-? ve TGF-? immunreaktiviteleri artarken PPAR-? ve PPAR-? immunreaktivitelerinde azalma dikkati çekti. Omega-3 takviyesinin incelenen biyokimyasal ve immunohistokimyasal parametreler üzerine olumlu etki gösterdiği belirlendi. Çalışma sonuçları omega-3'ün CCl4 uygulanan ratlarda oluşan oksidatif doku hasarı ve yangısal süreci azaltmada etkili olduğunu göstermektedir.

Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji

Ameliorative Effect of Omega-3 in Carbon Tetrachloride Toxicity

Öz:

Omega-3 is a polyunsaturated fatty acid known to have immunomodulatory functions. In the present study, ameliorative potential of omega-3 in experimental carbon tetrachloride (CCl4) toxicity was investigated. Total of 40 adult male Wistar albino rats were allocated into five groups and were subcutaneously given once every two days for 6 weeks the followings: Group 1 (Control): 0.5 ml/kg serum physiologic, Group 2 (Omega): 0.5 g/kg omega-3, Group 3 (Vehicle): 0.5 ml/kg pure olive oil, Group 4 (CCl4): 0.5 ml/kg CCl4, Group 5 (CCl4 + Omega): 0.5 ml/kg CCl4 plus 0.5 g/kg omega-3. At the end of the treatments, blood samples were collected and necropsy was performed for collection of liver tissues. Serum AST, AlT, GGT, TAC, TOC, triglyceride, and visfatin levels were detected. liver morphology and immunoreactivities against TGF-?, TGF-?, PPAR-?, and PPAR-? were assessed. Serum AST, AlT, GGT, and TOC levels significantly increased while TAC level decreased in CCl4 given animals as compared to the control group. No significant changes were observed in triglyceride and visfatin levels. Immunohistochemical staining revealed increased TGF-? and TGF-? expressions and decreased PPAR-? and PPAR-? expressions in liver of CCl4 given animals. Omega-3 supplementation has prominent effects in correcting the biochemical and immunohistochemical parameters studied as well as the tissue morphology. The results of the investigation indicated that omega-3 has ameliorative effects on the oxidative tissue degeneration and inflammatory processes induced by CCl4 treatment in rats.

Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KARAMAN M, ÖZEN H, DAĞ S, ATAKiŞİ O, Çığşar G, Kaya O (2017). Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi. , 77 - 85.
Chicago	KARAMAN Musa,ÖZEN Hasan,DAĞ Serpil,ATAKiŞİ Onur,Çığşar Gülşen,Kaya Oktay Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi. (2017): 77 - 85.
MLA	KARAMAN Musa,ÖZEN Hasan,DAĞ Serpil,ATAKiŞİ Onur,Çığşar Gülşen,Kaya Oktay Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi. , 2017, ss.77 - 85.
AMA	KARAMAN M,ÖZEN H,DAĞ S,ATAKiŞİ O,Çığşar G,Kaya O Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi. . 2017; 77 - 85.
Vancouver	KARAMAN M,ÖZEN H,DAĞ S,ATAKiŞİ O,Çığşar G,Kaya O Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi. . 2017; 77 - 85.
IEEE	KARAMAN M,ÖZEN H,DAĞ S,ATAKiŞİ O,Çığşar G,Kaya O "Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi." , ss.77 - 85, 2017.
ISNAD	KARAMAN, Musa vd. "Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi". (2017), 77-85.

APA	KARAMAN M, ÖZEN H, DAĞ S, ATAKiŞİ O, Çığşar G, Kaya O (2017). Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(1), 77 - 85.
Chicago	KARAMAN Musa,ÖZEN Hasan,DAĞ Serpil,ATAKiŞİ Onur,Çığşar Gülşen,Kaya Oktay Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 23, no.1 (2017): 77 - 85.
MLA	KARAMAN Musa,ÖZEN Hasan,DAĞ Serpil,ATAKiŞİ Onur,Çığşar Gülşen,Kaya Oktay Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.23, no.1, 2017, ss.77 - 85.
AMA	KARAMAN M,ÖZEN H,DAĞ S,ATAKiŞİ O,Çığşar G,Kaya O Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2017; 23(1): 77 - 85.
Vancouver	KARAMAN M,ÖZEN H,DAĞ S,ATAKiŞİ O,Çığşar G,Kaya O Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2017; 23(1): 77 - 85.
IEEE	KARAMAN M,ÖZEN H,DAĞ S,ATAKiŞİ O,Çığşar G,Kaya O "Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23, ss.77 - 85, 2017.
ISNAD	KARAMAN, Musa vd. "Karbon Tetraklorür Toksikasyonunda Omega-3'ün Koruyucu Etkisi". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 23/1 (2017), 77-85.